New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 453014550 (1.510)6 (0.190) 4/246 3.5 11.2% coding (99/1314 nt) sgcC putative PTS system EIIC permease component
?NC_000913 = 4530173 47 (1.490)coding (71/1314 nt) sgcC putative PTS system EIIC permease component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAG  >  NC_000913/4530027‑4530317
                                                                                                                                                  |                                                                                                                                                
gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGAt                                                                                                                                                 >  3:139452/1‑148 (MQ=255)
  aGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                                                              <  6:91145/149‑1 (MQ=255)
             gAAACGCTCAAACATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGa                                                                                                                                   >  3:1517/1‑149 (MQ=255)
                aCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCa                                                                                                                                <  1:157752/149‑1 (MQ=255)
                  gCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGCTCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCat                                                                                                                               >  5:151991/1‑148 (MQ=255)
                      aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                                                              <  8:52519/129‑1 (MQ=255)
                      aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                                                              >  7:52519/1‑129 (MQ=255)
                      aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCatgat                                                                                                                            <  4:139452/147‑1 (MQ=255)
                             aCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGAc                                                                                                                   >  5:6095/1‑149 (MQ=255)
                                               aGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATa                                                                                                                        <  7:120850/126‑1 (MQ=255)
                                               aGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATa                                                                                                                        >  8:120850/1‑126 (MQ=255)
                                                        aTCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                        >  2:142061/1‑101 (MQ=255)
                                                        aTCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                                                        <  1:142061/101‑1 (MQ=255)
                                                         tCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATg                                                                                       <  4:1517/149‑1 (MQ=255)
                                                            ccATGACGATCACCAGTCCCATAACGACAAAACCAATCACAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTaa                                                                                    >  8:22806/1‑149 (MQ=255)
                                                                   gATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAAc                                                                              >  4:106618/1‑148 (MQ=255)
                                                                       aCCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGTATGCGGAAGATAAGTACAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAAt                                                                         <  1:96007/149‑1 (MQ=255)
                                                                         cAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGc                                                                       >  1:123639/1‑149 (MQ=255)
                                                                                  aCCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTa                                                              >  3:90828/1‑149 (MQ=255)
                                                                                   ccGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTa                                                              <  6:6095/148‑1 (MQ=255)
                                                                                    agACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGTGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAACCGTTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAtt                                                            <  6:151991/148‑1 (MQ=255)
                                                                                         aaaCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACTGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        >  5:81831/1‑148 (MQ=255)
                                                                                         aaaCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        >  4:4732/1‑148 (MQ=255)
                                                                                           aCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGa                                                          <  8:118514/144‑1 (MQ=255)
                                                                                           aCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGa                                                          >  7:118514/1‑144 (MQ=255)
                                                                                             cAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATaa                                                                          <  2:74746/126‑1 (MQ=255)
                                                                                             cAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACa                                                   <  7:22806/149‑1 (MQ=255)
                                                                                                    aaCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        <  1:68460/137‑1 (MQ=255)
                                                                                                    aaCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        >  2:68460/1‑137 (MQ=255)
                                                                                                            ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACTGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt                                    <  6:81831/149‑1 (MQ=255)
                                                                                                            ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt                                    <  4:90828/149‑1 (MQ=255)
                                                                                                            ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt                                    >  6:49799/1‑149 (MQ=255)
                                                                                                             cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCtt                                            <  8:46531/140‑1 (MQ=255)
                                                                                                             cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCtt                                            >  7:46531/1‑140 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAAt                                                                                   <  4:1731/99‑1 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAAt                                                                                   >  3:1731/1‑99 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGtt                                                       <  2:160727/127‑1 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGtt                                                       >  1:160727/1‑127 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        <  1:8591/126‑1 (MQ=255)
                                                                                                               cTGCCTTAATCGCCTGAAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                        >  2:8591/1‑126 (MQ=255)
                                                                                                                tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACagag                                                <  6:3231/133‑1 (MQ=255)
                                                                                                                tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACagag                                                >  5:3231/1‑133 (MQ=255)
                                                                                                                        tCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAtt                                                            >  4:47824/1‑113 (MQ=255)
                                                                                                                          gCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGcgtcg                      >  8:34432/1‑149 (MQ=255)
                                                                                                                                 gCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGa               <  3:106618/149‑1 (MQ=255)
                                                                                                                                    aGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAAc                            <  2:12725/133‑1 (MQ=255)
                                                                                                                                    aGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAAc                            >  1:12725/1‑133 (MQ=255)
                                                                                                                                     ggggATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGAAGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAg                                                         <  7:61429/103‑1 (MQ=255)
                                                                                                                                             ggAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAg     >  1:78343/1‑147 (MQ=255)
                                                                                                                                              gAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAg  >  5:130523/1‑149 (MQ=255)
                                                                                                                                              gAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAg  >  1:65187/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGAGCAG  >  NC_000913/4530027‑4530317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.