Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,199,287 Δ1 bp 5.1% coding (355/2841 nt) glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,199,2870G. 5.1% 275.1 / 7.0 79coding (355/2841 nt)glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (41/34);  new base . (2/2);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAAC  >  NC_000913/3199140‑3199426
                                                                                                                                                   |                                                                                                                                           
gCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATagctgagc                                                                                                                                            <  1:148153/149‑1 (MQ=255)
  tCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATagctgagctg                                                                                                                                          >  6:73977/1‑149 (MQ=255)
    cccACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAgctgct                                                                                                                                        <  3:179202/149‑1 (MQ=255)
    cccACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAgctgct                                                                                                                                        >  8:275920/1‑149 (MQ=255)
    cccACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAgctgct                                                                                                                                        >  5:181893/1‑149 (MQ=255)
         cGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCaa                                                                                                                                    >  1:71865/1‑148 (MQ=255)
         cGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAat                                                                                                                                   <  4:197896/149‑1 (MQ=255)
          gCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAata                                                                                                                                  <  5:27832/149‑1 (MQ=255)
            ccAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATg                                                                                                                                <  5:337159/149‑1 (MQ=255)
                    cccATGCCTAAAATCAGGAGCGGGTGCGCTTCGCCCTGCGAATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGGCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCGGAGCTGCTGCAATATGCTCTCtt                                                                                                                         <  8:398092/148‑1 (MQ=255)
                    cccATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCtt                                                                                                                         <  5:242815/148‑1 (MQ=255)
                     ccATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCa                                                                                                                       <  8:104646/149‑1 (MQ=255)
                      cATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAg                                                                                                                      <  6:222242/149‑1 (MQ=255)
                       aTGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAg                                                                                                                      >  4:273321/1‑148 (MQ=255)
                          ccTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAAc                                                                                                                  >  7:183754/1‑149 (MQ=255)
                           cTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAAc                                                                                                                  >  4:24185/1‑148 (MQ=255)
                            tAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGctctc                                                                                                                           >  6:237096/1‑138 (MQ=255)
                            tAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGctctc                                                                                                                           <  5:237096/138‑1 (MQ=255)
                            tAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAAc                                                                                                                  >  1:193754/1‑147 (MQ=255)
                             aaaaTCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGA‑‑‑GCTGCAATATGCTCTCTTCAGTAACCAGTGc                                                                                                            >  4:49496/1‑149 (MQ=255)
                             aaaaTCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGA‑‑‑GCTGCAATATGCTCTCTTCAGTAACCAGTGc                                                                                                            >  4:54884/1‑149 (MQ=255)
                                 tCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGcc                                                                                                           >  2:418493/1‑149 (MQ=255)
                                   agcagcGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAg                                                                                                         <  4:144650/149‑1 (MQ=255)
                                           ttGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTgcg                                                                                                 >  3:31220/1‑149 (MQ=255)
                                               gcTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCCGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCGTCAGTAACCAGTGCCAGCGTTTGCGcc                                                                                               >  7:193691/1‑147 (MQ=255)
                                                   cGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAgctgct                                                                                                                                        >  5:303303/1‑102 (MQ=255)
                                                   cGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAgctgct                                                                                                                                        <  6:303303/102‑1 (MQ=255)
                                                     cccTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGAt                                                                                       <  4:70323/149‑1 (MQ=255)
                                                     cccTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGAt                                                                                       <  5:354848/149‑1 (MQ=255)
                                                          cgcATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATgcg                                                                                    <  8:183754/147‑1 (MQ=255)
                                                             aTTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCCCCa                                                                               >  2:49167/1‑149 (MQ=255)
                                                                  aCGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATg                                                                          >  7:92628/1‑149 (MQ=255)
                                                                   cGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATgc                                                                         <  2:226641/149‑1 (MQ=255)
                                                                           cccACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGccgccg                                                                  <  3:24185/148‑1 (MQ=255)
                                                                           cccACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGccgccg                                                                  <  3:150473/148‑1 (MQ=255)
                                                                            ccACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGccgccgc                                                                 >  8:18526/1‑147 (MQ=255)
                                                                            ccACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCgg                                                                 >  8:12360/1‑148 (MQ=255)
                                                                            ccACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGa                                                                <  2:71865/149‑1 (MQ=255)
                                                                              aCTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGaa                                                               >  2:294381/1‑148 (MQ=255)
                                                                              aCTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGaa                                                               >  2:291153/1‑148 (MQ=255)
                                                                                tCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAg                                                            >  8:147639/1‑149 (MQ=255)
                                                                                  gcgGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGcc                                                          >  2:45909/1‑149 (MQ=255)
                                                                                  gcgGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGA‑‑‑GCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCg                                                         <  3:49496/147‑1 (MQ=255)
                                                                                  gcgGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGA‑‑‑GCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCg                                                         <  3:54884/147‑1 (MQ=255)
                                                                                     gcagcaGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCa                                                       <  5:73977/149‑1 (MQ=255)
                                                                                       agcagGCGTCATCCAGCCAGTCACGCGCCGCAACAATCAGCGTCGCCGCCAGATACCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGc                                                     <  1:321464/149‑1 (MQ=255)
                                                                                         cagGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTc                                                   <  2:176110/149‑1 (MQ=255)
                                                                                           ggCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTcgc                                                 >  4:87900/1‑149 (MQ=255)
                                                                                           ggCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTcgc                                                 >  8:242776/1‑149 (MQ=255)
                                                                                                cATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAgg                                                                                           <  1:235012/102‑1 (MQ=255)
                                                                                                cATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAgg                                                                                           >  2:235012/1‑102 (MQ=255)
                                                                                                 aTACCGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCAtt                                            >  2:1833/1‑148 (MQ=255)
                                                                                                     aGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       >  1:329728/1‑149 (MQ=255)
                                                                                                     aGCCAGGCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTTCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       <  5:31217/149‑1 (MQ=255)
                                                                                                      gCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCg                                      >  8:57814/1‑149 (MQ=255)
                                                                                                           tCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTc                                 <  7:147639/149‑1 (MQ=255)
                                                                                                           tCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTc                                 <  2:193754/149‑1 (MQ=255)
                                                                                                             aCGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGt                               <  7:275920/149‑1 (MQ=255)
                                                                                                               gcgcCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCCGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtca                             >  1:22191/1‑149 (MQ=255)
                                                                                                               gcgcCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTg                                                                                                   <  5:170591/79‑1 (MQ=255)
                                                                                                               gcgcCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTg                                                                                                   >  6:170591/1‑79 (MQ=255)
                                                                                                               gcgcCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtca                             >  3:90055/1‑149 (MQ=255)
                                                                                                                 gccgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       <  4:29598/137‑1 (MQ=255)
                                                                                                                 gccgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       >  3:29598/1‑137 (MQ=255)
                                                                                                                 gccgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcact                           >  5:219677/1‑149 (MQ=255)
                                                                                                                 gccgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcact                           <  1:291153/149‑1 (MQ=255)
                                                                                                                   cgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactca                         >  4:354224/1‑149 (MQ=255)
                                                                                                                   cgcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactca                         <  1:45909/149‑1 (MQ=255)
                                                                                                                    gcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactca                         >  3:152800/1‑148 (MQ=255)
                                                                                                                     caacaaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTcaca                       >  2:126331/1‑149 (MQ=255)
                                                                                                                     caacaaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTcaca                       >  2:301180/1‑149 (MQ=255)
                                                                                                                         aaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       <  8:423546/129‑1 (MQ=255)
                                                                                                                         aaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAAccc                                       >  7:423546/1‑129 (MQ=255)
                                                                                                                         aaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTa                    >  2:382147/1‑148 (MQ=255)
                                                                                                                           tCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTa                    >  7:421910/1‑146 (MQ=255)
                                                                                                                           tCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACag                 >  6:189236/1‑149 (MQ=255)
                                                                                                                             aGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACagag               >  3:268092/1‑149 (MQ=255)
                                                                                                                               cGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcg             >  3:299444/1‑149 (MQ=255)
                                                                                                                                 tctcCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcc           >  8:16428/1‑149 (MQ=255)
                                                                                                                                    ccGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcctcc        >  5:233747/1‑149 (MQ=255)
                                                                                                                                     cGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcctcc        <  7:18526/148‑1 (MQ=255)
                                                                                                                                     cGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcctcc        <  7:12360/148‑1 (MQ=255)
                                                                                                                                     cGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcctc         <  4:152800/147‑1 (MQ=255)
                                                                                                                                      gCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTg      >  5:54459/1‑149 (MQ=255)
                                                                                                                                         aGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCTCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCaa   <  1:418493/149‑1 (MQ=255)
                                                                                                                                          gATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAAc  <  3:273321/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GCTCCCCACCGCCCAGCTTACCCATGCCTAAAATCAGCAGCGGTTGCGCTTCGCCCTGCGCATTGCACGGCGTTCCCCACTCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAAC  >  NC_000913/3199140‑3199426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑