Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,966 C→T 8.8% H354Y (CAC→TAC)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,9660CT 8.8% 234.0 / 9.9 79H354Y (CAC→TAC) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (37/35);  new base T (3/4);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGG  >  NC_000913/756820‑757111
                                                                                                                                                  |                                                                                                                                                 
gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGCTGAAAGAGCCGATTCCGGTATCCCAACCTGTCAc                                                                                                                                                 <  6:71825/148‑1 (MQ=255)
 tCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                                                                                                                 >  7:167434/1‑148 (MQ=255)
  ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTa                                                                                                                                               >  1:9022/1‑149 (MQ=255)
         ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGGCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgatg                                                                                                                                        <  7:83600/149‑1 (MQ=255)
           ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATggg                                                                                                                                      >  5:89465/1‑149 (MQ=255)
           ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATggg                                                                                                                                      >  5:3116/1‑149 (MQ=255)
             acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGCGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                                                                                                                    >  5:72802/1‑149 (MQ=255)
                cgcgAAACTGAAACTCGAGCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTa                                                                                                                                 <  8:200428/149‑1 (MQ=255)
                  cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTAtt                                                                                                                               >  2:406298/1‑149 (MQ=255)
                   gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                                                                                                              >  2:302856/1‑149 (MQ=255)
                       ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                                                                                                          <  8:238520/149‑1 (MQ=255)
                              tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa                                                                                                                      >  8:37096/1‑146 (MQ=255)
                              tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa                                                                                                                      <  7:37096/146‑1 (MQ=255)
                                gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                                                                                                 >  2:416750/1‑149 (MQ=255)
                                gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                                                                                                 <  2:248307/149‑1 (MQ=255)
                                  tCACCTGGGTAAAGAAGTTCTCTAATCCCGTCTGCCTGGTATCCTGGAGCTTTCGCTTACCTTCGCTCACGTGGTTCCGTTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACTTGATGGGCGGTATTCCGACCAAAGTTACCg                                                                                                               <  3:96289/149‑1 (MQ=255)
                                     ccTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGt                                                                                                             >  7:381459/1‑148 (MQ=255)
                                         ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                                                                                                         <  4:356657/148‑1 (MQ=255)
                                         ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTATTCCGACCAAAGTTACCGGTCAGGc                                                                                                        <  1:406298/149‑1 (MQ=255)
                                         ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGACGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTATTCCGACCAAAGTTACCGGTCAgg                                                                                                         >  4:168544/1‑148 (MQ=255)
                                            aaaGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCact                                                                                                     >  2:242280/1‑149 (MQ=255)
                                               gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac                                                                                                  >  8:339310/1‑149 (MQ=255)
                                               gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac                                                                                                  >  5:315101/1‑149 (MQ=255)
                                                  gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                                                                                               <  4:369407/149‑1 (MQ=255)
                                                  gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                                                                                               <  1:302856/149‑1 (MQ=255)
                                                   ttCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                                                                                               >  7:329922/1‑148 (MQ=255)
                                                     ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa                                                                                             <  6:3116/148‑1 (MQ=255)
                                                     ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGGCAGGCACTGACTGTGa                                                                                             <  6:89465/148‑1 (MQ=255)
                                                        gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAAt                                                                                           <  8:381459/147‑1 (MQ=255)
                                                           tCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga                                                                                       >  8:306534/1‑148 (MQ=255)
                                                             ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa                                                                                     <  6:315101/148‑1 (MQ=255)
                                                             ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa                                                                                     <  7:306534/148‑1 (MQ=255)
                                                             ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAg                                                                                    <  3:384134/149‑1 (MQ=255)
                                                                tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa                                                                                             >  6:409563/1‑137 (MQ=255)
                                                                tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa                                                                                             <  5:409563/137‑1 (MQ=255)
                                                                    ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATTATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg                                                                                 >  6:130438/1‑145 (MQ=255)
                                                                    ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg                                                                                 >  6:399291/1‑145 (MQ=255)
                                                                    ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg                                                                                 >  8:375686/1‑145 (MQ=255)
                                                                    ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCACAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg                                                                                 >  8:29375/1‑145 (MQ=255)
                                                                         tATCCTGGAGCTTTCCCGTCCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                        <  2:289337/149‑1 (MQ=255)
                                                                         tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                        <  4:82851/149‑1 (MQ=255)
                                                                         tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                        <  1:242280/149‑1 (MQ=255)
                                                                           tCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgt                                                                       <  7:339310/148‑1 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAt                                                                            >  1:154273/1‑140 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAt                                                                            <  2:154273/140‑1 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                   >  6:123159/1‑149 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                   >  4:31095/1‑149 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                   >  4:24517/1‑149 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                   >  2:228791/1‑149 (MQ=255)
                                                                              tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt                                                                   >  2:222101/1‑149 (MQ=255)
                                                                                gAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTcc                                                                 >  7:347658/1‑149 (MQ=255)
                                                                                   cTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCgg                                                               >  6:90161/1‑148 (MQ=255)
                                                                                    tttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGa                                                             >  7:284182/1‑149 (MQ=255)
                                                                                     ttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGAc                                                            >  5:181915/1‑149 (MQ=255)
                                                                                        ccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                        >  1:158536/1‑134 (MQ=255)
                                                                                        ccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg                                                                        <  2:158536/134‑1 (MQ=255)
                                                                                        ccGTACCTTCGCGCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGTGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGt                                                         <  8:150842/149‑1 (MQ=255)
                                                                                          gTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCCACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGttt                                                       <  2:96520/149‑1 (MQ=255)
                                                                                               ttCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTTCCGt                                                  <  1:228791/149‑1 (MQ=255)
                                                                                                 cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGt                                                  <  1:222101/147‑1 (MQ=255)
                                                                                                 cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGt                                                  <  3:31095/147‑1 (MQ=255)
                                                                                                  gCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg                                               <  5:130438/149‑1 (MQ=255)
                                                                                                  gCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTg                                                <  1:171418/148‑1 (MQ=255)
                                                                                                      aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg                                               <  1:214320/145‑1 (MQ=255)
                                                                                                      aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg                                               >  2:214320/1‑145 (MQ=255)
                                                                                                        gTCGCTCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAAt                                         >  6:119094/1‑149 (MQ=255)
                                                                                                               cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCTGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgt                                  <  8:347658/149‑1 (MQ=255)
                                                                                                                ggTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg                                 >  4:16107/1‑149 (MQ=255)
                                                                                                                       gagCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGt                          <  5:90161/149‑1 (MQ=255)
                                                                                                                         gCCGATTCCGGTTATCACAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTac                        <  7:29375/149‑1 (MQ=255)
                                                                                                                            gATTCCGGTTATCCCAACCTGTTACTACATGATGTGCGGTATTCCGACCAAAGTTACCGTTCAGGCAATGACTGAGAATGAGAAAGGCGAAGATGTGGTTGTTACGGGACTGTTTCCCGATGCTGaaa                                          >  8:76950/1‑128 (MQ=255)
                                                                                                                            gATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGaaa                                          <  7:76950/128‑1 (MQ=255)
                                                                                                                             aTTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACgg                    >  3:378569/1‑149 (MQ=255)
                                                                                                                                       tCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGt          >  7:183791/1‑149 (MQ=255)
                                                                                                                                         ccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTc         <  3:16107/148‑1 (MQ=255)
                                                                                                                                           aaCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTgg      <  8:284182/149‑1 (MQ=255)
                                                                                                                                              cTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcg   >  4:345642/1‑149 (MQ=255)
                                                                                                                                                gTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcgg  >  6:11374/1‑148 (MQ=255)
                                                                                                                                                 tCACTACATGATGGGCGGTTTTCCGACCAAAGTAACCGGTCAGGCACTGACTGTGAATGAGAAAGGATTTGATGTTGTTGTTCCGGGACTGTTTGCCTTTGGTTAAATCTCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcgg  <  6:66441/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGG  >  NC_000913/756820‑757111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: