| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,586,450 | C→A | 6.4% | L240L (CTC→CTA) | narQ → | sensory histidine kinase in two‑component regulatory system with NarP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,586,450 | 0 | C | A | 6.4% | 267.5 / 2.1 | 85 | L240L (CTC→CTA) | narQ | sensory histidine kinase in two‑component regulatory system with NarP |
| Reads supporting (aligned to +/- strand): ref base C (49/29); new base A (2/4); total (51/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCGGTGAA > NC_000913/2586305‑2586597 | acGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAACCCTTTAAGCAGATGTCGAGCGAGCTGCATAAATTGTAGCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCAc > 1:270583/1‑149 (MQ=255) gggCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCAc > 1:362166/1‑147 (MQ=255) gCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAgg > 6:382909/1‑149 (MQ=255) ttCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCATGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCCTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGAACTCCACGAGGCCaa > 2:204190/1‑149 (MQ=255) gACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGCGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGcg > 1:350972/1‑149 (MQ=255) gACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGcg > 4:295283/1‑149 (MQ=255) gccgccgcTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCgtcgtc > 8:371494/1‑149 (MQ=255) ccgcTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGa < 7:368897/149‑1 (MQ=255) gATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGtgtt < 7:59298/149‑1 (MQ=255) ccTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTAAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCTATTTTATTTTAATTATAAGTATAGAACCGAGATCTACACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAgt < 6:199664/149‑1 (MQ=255) gAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGTGCTGCATAAATTTTACCTTTCTTTTTTTTTTTAATTATAAGTATAGAACCGAGATCTTCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGc < 6:296843/149‑1 (MQ=255) gAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCg > 4:108959/1‑148 (MQ=255) aaTGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGCTGTTGTATCAGTGTTca > 2:16270/1‑146 (MQ=255) aaTGAGCTTGGTCTGATTGCAAAAACCTTTAACAAGATGTCGAGCGAGCTCAATAAATTGTACCGTTCGCTGGAAGCTTCAGTAGAAGAAAAGACCCGCGATATCCACAAGGCAAATCGACGACTGGATGTGTTCTATCAGTCTTcca > 2:9299/1‑146 (MQ=255) aTGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAg > 3:224387/1‑149 (MQ=255) aGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGgcg > 5:130780/1‑149 (MQ=255) aGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTACACGATGCCAAGCGTCGTCAGGAGGTGTTGTATCAGTGTTCGCAGgcg > 5:62907/1‑149 (MQ=255) gCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCTAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTCTTGTATCAGAGTTCGCAGgcgc > 2:147168/1‑149 (MQ=255) gCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGgcgc > 8:82236/1‑149 (MQ=255) cTTGGTCTGCTTTCAAAAACATTTAACCAGATGTCGATCGAGCTTCATAAATTGTACCGTTCGTTTGTTTTTTAATTATAAGTATAGACCCGAGATCTACACGAGGCCAAGAGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCt < 6:62907/149‑1 (MQ=255) cTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGgcg < 3:261429/147‑1 (MQ=255) cTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGgcg > 4:261429/1‑147 (MQ=255) ttGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGTGTCGTCTGGAGGtgttg < 7:90723/128‑1 (MQ=255) ttGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGTGTCGTCTGGAGGtgttg > 8:90723/1‑128 (MQ=255) ggTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGGTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCACGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGaa > 6:231070/1‑149 (MQ=255) gTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGaa > 3:285333/1‑148 (MQ=255) gTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAACCGTCGTCTGGAGGTGTTGAATCAGTGTTCGCAGGCGCTGaa > 2:400852/1‑148 (MQ=255) tGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGATCGCTGGAAGCGTCAGTATAAGAAAAGACCCCCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACt < 1:204190/149‑1 (MQ=255) gCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGgtgt > 7:206428/1‑119 (MQ=255) gCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGgtgt < 8:206428/119‑1 (MQ=255) cTTTCAAAAACCTTTAACCAGATGTAGAGCGAGGTTCATAAATTTTACCGTTAGATTGAATAGTAATTAGAAGAATAGAACCGAGATCTACACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTTAACACTa < 2:270583/148‑1 (MQ=255) cTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACt < 7:82236/147‑1 (MQ=255) ttGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGc > 6:214369/1‑149 (MQ=255) gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGc > 4:135884/1‑147 (MQ=255) gCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGc > 8:303474/1‑147 (MQ=255) aaaaaCCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAATAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGa < 4:285333/149‑1 (MQ=255) aaaaaCCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGa < 5:382909/149‑1 (MQ=255) aaaaaCCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACAGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCAACGAGGCCAAGCTTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCAGAACACTAGc > 1:103196/1‑145 (MQ=255) aaaaCCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGAt > 8:347996/1‑149 (MQ=255) aaaaCCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTAGTTTTTTTTTTAATTATAAGTATAGCACCGAGATCTACACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGAt < 4:364660/149‑1 (MQ=255) ccTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGAt < 6:130780/149‑1 (MQ=255) ccTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGAGTTCGCAGGCGCTGAACACTAGCCAGATTGAt < 1:400852/149‑1 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGAtg > 7:216844/1‑149 (MQ=255) cTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGAtg > 2:24824/1‑149 (MQ=255) tttAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGAACCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGTCGCTGAACACTAGCCAGATTGAtgt > 6:85242/1‑149 (MQ=255) tAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGAtgt < 1:24824/147‑1 (MQ=255) aCCAGATGTCGAGCGAGCTGCAGAAATTGTACCGTTCGCTGGCAGCGTCAGTAGAAGAAAAGACCCGCGATCTAAACGAGTCCAAGCGGCTTCTGGAGTTGTTGTATCAATGTTCGCAGGCGCTGAACACTAGCCAGAATTATGTTCAt > 8:293849/1‑149 (MQ=255) ccAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCAtt > 1:114860/1‑149 (MQ=255) gcgagcTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCat < 8:216844/149‑1 (MQ=255) gagcTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCACGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGCACACTAGCCAGATTGATGTGCATTGTTTCCGCCatat > 4:345931/1‑149 (MQ=255) agcTGCATAATTTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATAtt < 5:66954/149‑1 (MQ=255) agcTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATAtt < 5:214369/149‑1 (MQ=255) agcTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATAtt > 3:134484/1‑149 (MQ=255) agcTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATAtt > 4:174997/1‑149 (MQ=255) cATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCa > 7:131785/1‑149 (MQ=255) cATAAATTGTACAGTTCGCTTGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGTAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCATATTGATGTGCATTGTTTCCTCCATATTTTGCa < 5:85242/149‑1 (MQ=255) tACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGCACACTAGCCAGCTTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTcg > 1:396722/1‑149 (MQ=255) tACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTcg > 5:284596/1‑149 (MQ=255) tACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTcg > 8:196328/1‑149 (MQ=255) ccGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTcgc > 3:127845/1‑148 (MQ=255) ttCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGAc > 1:295630/1‑148 (MQ=255) gCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAAt < 2:396722/148‑1 (MQ=255) gtaGCTTCAGTAGAAGAAAAGACCCGCTATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAg < 4:127845/146‑1 (MQ=255) gAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGaa < 1:147168/147‑1 (MQ=255) gAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAg < 3:135884/148‑1 (MQ=255) gAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGc > 4:177076/1‑149 (MQ=255) gTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATAAGTGTTCGAAGGCGCTGAACACTAGCCAGAGTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGGTCGCGCCAATGAAGCGGCTGAata > 8:119218/1‑149 (MQ=255) aagaaAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATc > 2:145298/1‑147 (MQ=255) aagaaAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATc < 1:145298/147‑1 (MQ=255) aagaaAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTg < 7:347996/149‑1 (MQ=255) gaaAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATAtgtg > 6:2805/1‑147 (MQ=255) gaaAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGa < 4:134484/149‑1 (MQ=255) aaaGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGtt > 8:60414/1‑118 (MQ=255) aaaGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGtt < 7:60414/118‑1 (MQ=255) aaGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGtt < 5:231070/149‑1 (MQ=255) cccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAAt < 3:295283/149‑1 (MQ=255) cccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAAt > 8:330255/1‑149 (MQ=255) cccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGAAAGAGTGATGTGCATTGTTTCCTCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAAt > 8:264847/1‑149 (MQ=255) ccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCt < 2:192820/130‑1 (MQ=255) ccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCt > 1:192820/1‑130 (MQ=255) ccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATg > 1:403840/1‑149 (MQ=255) ccGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGAAAATGAAGCGGCTGAATATCTGCAGTTAAATg > 5:29102/1‑149 (MQ=255) cgATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCg < 3:85220/149‑1 (MQ=255) cgATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCg < 3:108959/149‑1 (MQ=255) aTCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCGGt > 1:361145/1‑149 (MQ=255) tcCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGa < 6:148189/124‑1 (MQ=255) tcCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGa > 5:148189/1‑124 (MQ=255) ccACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCGGTGaa > 3:147190/1‑148 (MQ=255) | ACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAACCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTCCACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTTCCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCGGTGAA > NC_000913/2586305‑2586597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |