Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	755,767	Δ1 bp	coding (208/348 nt)	sdhD →	succinate dehydrogenase, membrane subunit, binds cytochrome b556

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	755,767	0	A	.	100.0%	405.0 / NA	89	coding (208/348 nt)	sdhD	succinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (45/44);  total (45/44)

CGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGAT  >  NC_000913/755625‑755908                                                                                                                                               |                                                                                                                                              cGCTATCGTCCTGACTCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGc                                                                                                                                        <  5:278596/149‑1 (MQ=255) cGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGc                                                                                                                                        >  5:259768/1‑149 (MQ=255) cGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGc                                                                                                                                        >  1:299255/1‑149 (MQ=255)  gCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGcc                                                                                                                                       <  5:173641/149‑1 (MQ=255)   cTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCt                                                                                                                                      <  4:235330/149‑1 (MQ=255)   cTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCt                                                                                                                                      <  6:204824/149‑1 (MQ=255)      tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGGCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTg                                                                                                                                     <  4:347669/147‑1 (MQ=255)        gTCCTGAAGTTCTACATCATTTATATGGTCTTTTTTTTCGCTACCAGTGGAGAGCTGACATATGAAGTCTGGATCTGTTTCTTCGCTTCTGATTTCACCAAAGTGTTCACCCTGCTGTCGCTGTTTTCTATCTTG‑TCCATGCATTGAt                                                                                                                                  <  6:348800/148‑1 (MQ=255)           cTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCgg                                                                                                                               >  7:251967/1‑148 (MQ=255)           cTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGc                                                                                                                              <  5:173692/149‑1 (MQ=255)             gACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTCTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCAt                                                                                                                            >  6:121368/1‑149 (MQ=255)                gCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCAtgtg                                                                                                                         >  3:199757/1‑149 (MQ=255)                gCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCAtgtg                                                                                                                         >  7:61162/1‑149 (MQ=255)                 ctctACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTTGATCGGTTTCTTCGCCTCAGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑GCCATGCCTGGATCGGCATGTgg                                                                                                                        <  6:75776/149‑1 (MQ=255)                    tACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGTCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGc                                                                                                                       <  1:86171/147‑1 (MQ=255)                      catcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCa                                                                                                                      <  8:251967/146‑1 (MQ=255)                       atcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtg                                                                                                                  <  6:259768/149‑1 (MQ=255)                       atcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtg                                                                                                                  >  7:91468/1‑149 (MQ=255)                        tcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtgt                                                                                                                 >  4:323315/1‑149 (MQ=255)                        tcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGAGAGGCATGTGGCAGgtgt                                                                                                                 >  2:284762/1‑149 (MQ=255)                        tcatTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTACGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtgt                                                                                                                 >  4:376642/1‑149 (MQ=255)                        tcatTTATATGGTCGGTTTATTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtgt                                                                                                                 >  2:58743/1‑149 (MQ=255)                             tataTGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGAc                                                                                                             <  2:264107/148‑1 (MQ=255)                                    tCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACg                                                                                                     >  7:397070/1‑149 (MQ=255)                                    tCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACg                                                                                                     >  1:153216/1‑149 (MQ=255)                                     cGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGt                                                                                                    <  8:61162/149‑1 (MQ=255)                                        ttttttCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTa                                                                                                  >  7:233924/1‑148 (MQ=255)                                          tcttCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAAc                                                                                               >  6:168895/3‑149 (MQ=255)                                            ttCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCg                                                                                             >  7:114219/1‑149 (MQ=255)                                               gCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCt                                                                                           <  7:415916/148‑1 (MQ=255)                                               gCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCCGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTg                                                                                          >  3:251257/1‑149 (MQ=255)                                                 tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtg                                                                                                                  >  5:213854/1‑123 (MQ=255)                                                 tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGgtg                                                                                                                  <  6:213854/123‑1 (MQ=255)                                                 tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGa                                                                                                          <  6:390650/131‑1 (MQ=255)                                                 tACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCAGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGa                                                                                                          >  5:390650/1‑131 (MQ=255)                                                   ccAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCt                                                                                       <  3:323315/148‑1 (MQ=255)                                                      gTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTgc                                                                                   <  3:114571/149‑1 (MQ=255)                                                          cGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCt                                                                               <  6:279281/149‑1 (MQ=255)                                                               tGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgc                                                                          >  2:222473/1‑149 (MQ=255)                                                                 aCATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                                        >  3:54982/1‑149 (MQ=255)                                                                 aCATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgct                                                                         <  6:75211/148‑1 (MQ=255)                                                                     atGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                                        <  7:202830/145‑1 (MQ=255)                                                                     atGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctg                                                                        >  8:202830/1‑145 (MQ=255)                                                                        aaGTCTGGCTCGGTTCCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGGTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTGGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAAc                                                                    <  6:185820/146‑1 (MQ=255)                                                                        aaGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTg                                                                  <  2:228762/148‑1 (MQ=255)                                                                         aGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGt                                                                <  7:203400/149‑1 (MQ=255)                                                                                aTCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTc                                                         >  2:262948/1‑149 (MQ=255)                                                                                aTCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCGGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTc                                                         <  6:294803/149‑1 (MQ=255)                                                                                 tCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCg                                                        >  1:188764/1‑149 (MQ=255)                                                                                   ggTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGtt                                                      <  1:262948/149‑1 (MQ=255)                                                                                   ggTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGt                                                       <  1:222473/148‑1 (MQ=255)                                                                                       tcttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCAc                                                  >  4:24136/1‑149 (MQ=255)                                                                                         ttcGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGAATACGTTAAACCGCTGGCTTTTCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtg                                                >  2:34261/1‑149 (MQ=255)                                                                                            gCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCtt                                                                                      <  6:120950/108‑1 (MQ=255)                                                                                            gCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCtt                                                                                      >  5:120950/1‑108 (MQ=255)                                                                                            gCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACtggtg                                             >  3:150018/1‑149 (MQ=255)                                                                                                 tGCGTTCACCAAAGTGTTCACCCTGCTGGCTCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGCCCGACTACGGTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGAGATTGTCGTTGCACTGGTGGTTTa                                        >  8:366746/1‑149 (MQ=255)                                                                                                   cGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCttt                                                                                     <  8:170639/102‑1 (MQ=255)                                                                                                   cGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCttt                                                                                     >  7:170639/1‑102 (MQ=255)                                                                                                   cGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACg                                      <  2:299255/149‑1 (MQ=255)                                                                                                    gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGt                                     >  5:197188/1‑149 (MQ=255)                                                                                                    gTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGt                                     <  4:150018/149‑1 (MQ=255)                                                                                                     ttCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGt                                     >  1:180734/1‑148 (MQ=255)                                                                                                        aCCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGAtt                                 <  3:76327/149‑1 (MQ=255)                                                                                                         ccAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctgc                                                                       <  2:261371/110‑1 (MQ=255)                                                                                                         ccAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgctgc                                                                       >  1:261371/1‑110 (MQ=255)                                                                                                          cAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTa                               >  6:162907/1‑149 (MQ=255)                                                                                                              gtgtTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGa                           <  4:199757/149‑1 (MQ=255)                                                                                                               tgtTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGAt                          >  1:126804/1‑149 (MQ=255)                                                                                                                gtTCACCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGAtt                         >  1:357719/1‑149 (MQ=255)                                                                                                                    agccTGCTGGCGCTGGTTTCTCTCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt                     <  3:31537/147‑1 (MQ=255)                                                                                                                    aCCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt                     >  1:137835/1‑149 (MQ=255)                                                                                                                    aCCCTGCTGGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGtt                     <  6:333251/149‑1 (MQ=255)                                                                                                                       ctgctgGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtg                  <  2:180734/149‑1 (MQ=255)                                                                                                                          ctgGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGt                                                       >  2:271124/1‑109 (MQ=255)                                                                                                                          ctgGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGt                                                       <  1:271124/109‑1 (MQ=255)                                                                                                                          ctgGCGCTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggt                <  4:245443/148‑1 (MQ=255)                                                                                                                             gcgcTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgtggtgtg             <  4:30278/148‑1 (MQ=255)                                                                                                                              cgcTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTggg           >  6:294670/1‑149 (MQ=255)                                                                                                                                cTGTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgt         <  5:121368/149‑1 (MQ=255)                                                                                                                                  gTTTTCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGCCCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgt       >  4:15821/1‑149 (MQ=255)                                                                                                                                   ttttCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGgtgt       <  5:168895/148‑1 (MQ=255)                                                                                                                                     ttCTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACTTTAAAAAGCGGGCTTTGCGCCGGATGCTGCAACTGGTGATTGTCGTTGCAATGGTGGTTTACGTGATTTTTGGATTCGTTGTTGTGTGGGgtgtgt     >  6:319183/1‑148 (MQ=255)                                                                                                                                       cTATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGCCCGACTACGTTAAGCCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgat  >  6:141186/1‑149 (MQ=255)                                                                                                                                        tATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgct                                                                         >  6:193172/1‑77 (MQ=255)                                                                                                                                        tATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGAtgct                                                                         <  5:193172/77‑1 (MQ=255)                                                                                                                                        tATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgt                   >  2:8645/1‑131 (MQ=255)                                                                                                                                        tATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGtgat  >  3:118903/1‑148 (MQ=255)                                                                                                                                        tATCTTG‑TCCATGCCTGGATCGGCATGTGGCAGGGGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTtgt                   <  1:8645/131‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              CGCTATCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGTCTGGATCGGTTTCTTCGCCTCTGCGTTCACCAAAGTGTTCACCCTGCTGGCGCTGTTTTCTATCTTGATCCATGCCTGGATCGGCATGTGGCAGGTGTTGACCGACTACGTTAAACCGCTGGCTTTGCGCCTGATGCTGCAACTGGTGATTGTCGTTGCACTGGTGGTTTACGTGATTTATGGATTCGTTGTGGTGTGGGGTGTGTGAT  >  NC_000913/755625‑755908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑