Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,964,929	C→T	E234E (GAG→GAA)	rhlB ←	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,964,929	0	C	T	100.0%	237.8 / NA	68	E234E (GAG→GAA)	rhlB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/31);  total (37/31)

CCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCG  >  NC_000913/3964792‑3965071                                                                                                                                          |                                                                                                                                               ccATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAAt                                                                                                                                     <  5:103947/149‑1 (MQ=255)  cATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATa                                                                                                                                    <  5:121833/149‑1 (MQ=255)    tCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACg                                                                                                                                  >  5:56803/1‑149 (MQ=255)        ctgcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTgg                                                                                                                              >  5:32392/1‑149 (MQ=255)          gcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGcc                                                                                                                            >  5:106341/1‑149 (MQ=255)               gTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGttt                                                                                                                       <  6:32392/149‑1 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  6:72497/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  3:28984/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  6:75800/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  3:74206/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  5:29523/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  1:130408/1‑149 (MQ=255)                    cccAGATCTCTTCACAACGGTGTTAGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt                                                                                                                  >  6:128029/1‑149 (MQ=255)                     ccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGtt                                                                                                                 >  3:38133/1‑149 (MQ=255)                              ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCgg                                                                                                             <  6:29523/144‑1 (MQ=255)                               tCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCAc                                                                                                       >  7:66605/1‑149 (MQ=255)                                cacaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACt                                                                                                      >  5:76964/1‑149 (MQ=255)                                 acaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt                                                                                                     <  5:72497/149‑1 (MQ=255)                                   aaCGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTc                                                                                                    >  3:32591/1‑148 (MQ=255)                                     cGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAat                                                                                                 <  4:74206/149‑1 (MQ=255)                                                 ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGc                                                                                        >  7:25598/1‑146 (MQ=255)                                                 ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGc                                                                                        <  8:25598/146‑1 (MQ=255)                                                    gCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGt                                                                                                                         <  3:39811/110‑1 (MQ=255)                                                    gCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGt                                                                                                                         >  4:39811/1‑110 (MQ=255)                                                       aaaaTAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtctt                                                                                                                                            <  7:95491/88‑1 (MQ=255)                                                       aaaaTAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtctt                                                                                                                                            >  8:95491/1‑88 (MQ=255)                                                        aaaTAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATc                                                                              <  6:56803/149‑1 (MQ=255)                                                           taatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGc                                                                           <  4:32591/149‑1 (MQ=255)                                                            aatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCt                                                                          >  8:64631/1‑149 (MQ=255)                                                             atCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc                                                                         <  4:28984/149‑1 (MQ=255)                                                                         ggCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGtt                                                              >  5:19926/1‑148 (MQ=255)                                                                            cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                          >  5:115969/1‑149 (MQ=255)                                                                             aCTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCAt                                                                                      <  8:97504/120‑1 (MQ=255)                                                                                 ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCTGCATTGTTCATCTGCTCGAACTCCAGTTCACGTAccc                                                     >  8:26235/1‑149 (MQ=255)                                                                                 ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAccc                                                     >  3:101742/1‑149 (MQ=255)                                                                                 ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAc                                                       <  5:75800/147‑1 (MQ=255)                                                                                   cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGTGTAGAAAAGTTATTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCgg                                                   >  3:92406/1‑149 (MQ=255)                                                                                   cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAccc                                                     <  7:64631/147‑1 (MQ=255)                                                                                    ttcGATCAGCGGTTGCAGCAAACGCATTTTTTCTTAGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGTTTCCACTTAAATATATTCGGCATTGATCATCTGCTCGAACGCCAGTTCACGTACCCgg                                                   >  3:41755/1‑148 (MQ=255)                                                                                           aGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa                                            <  4:38133/148‑1 (MQ=255)                                                                                           aGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa                                            <  6:76964/148‑1 (MQ=255)                                                                                            gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTgg                                                                                                                              <  1:60241/65‑1 (MQ=255)                                                                                            gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTgg                                                                                                                              >  2:60241/1‑65 (MQ=255)                                                                                                 tGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTgg                                      <  6:106341/148‑1 (MQ=255)                                                                                                  gcagcaAACGCATTTTTTCTTCGTTAGTAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGGTCGACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                     <  5:128029/148‑1 (MQ=255)                                                                                                  gcagcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGc                                     <  4:101742/148‑1 (MQ=255)                                                                                                    agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCg                                    >  2:133605/1‑147 (MQ=255)                                                                                                        aaCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTACACGTACCCGGTACGAAAGCGTGGCGGAGaa                               >  1:138432/1‑148 (MQ=255)                                                                                                         aCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGaa                               >  1:137765/1‑147 (MQ=255)                                                                                                          cGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAg                            <  6:19926/149‑1 (MQ=255)                                                                                                            cATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCa                          <  6:115969/149‑1 (MQ=255)                                                                                                                  ttcttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGtt                      >  6:102046/1‑147 (MQ=255)                                                                                                                     ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGtt                      <  1:100621/144‑1 (MQ=255)                                                                                                                     ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGtt                      >  2:100621/1‑144 (MQ=255)                                                                                                                     ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc                 >  2:97810/1‑149 (MQ=255)                                                                                                                       cGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACt                                                                                                      >  3:18911/1‑62 (MQ=255)                                                                                                                       cGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACt                                                                                                      <  4:18911/62‑1 (MQ=255)                                                                                                                       cGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc                 <  5:102046/147‑1 (MQ=255)                                                                                                                         ttAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGa                    >  7:102753/1‑142 (MQ=255)                                                                                                                         ttAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGa                    <  8:102753/142‑1 (MQ=255)                                                                                                                         ttAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTg             <  8:66605/149‑1 (MQ=255)                                                                                                                               gggTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTg        >  5:91493/1‑148 (MQ=255)                                                                                                                               gggTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTg        >  3:47447/1‑148 (MQ=255)                                                                                                                               gggTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTg        >  3:49550/1‑148 (MQ=255)                                                                                                                               gggTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGc       <  1:97810/149‑1 (MQ=255)                                                                                                                                   aGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCa      >  2:102440/1‑146 (MQ=255)                                                                                                                                   aGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCa      <  1:102440/146‑1 (MQ=255)                                                                                                                                    gAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAggcg  <  1:133605/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                               CCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCG  >  NC_000913/3964792‑3965071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑