Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)21 (0.660) 20/270 0.7 91.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 2 (0.070)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)35 (1.110) 31/268 0.2 94.4% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 2 (0.070)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

AAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910942‑911238
                                                                                                                                                    |                                                                                                                                                    
aaGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAg                                                                                                                                                      >  4:24936/1‑149 (MQ=255)
 aGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGt                                                                                                                                                     >  6:54746‑M2/1‑148 (MQ=255)
            aaGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccc                                                                                                                                          <  4:86666‑M2/149‑13 (MQ=255)
             gacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGa                                                                                                                                         <  2:61750‑M2/18‑1 (MQ=255)
             aGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccc                                                                                                                                          <  3:94519‑M2/148‑13 (MQ=255)
             aGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgccc                                                                                                                                           >  7:32920‑M2/1‑136 (MQ=255)
               aCCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctat                                                                                                                                       >  3:86666‑M2/1‑134 (MQ=255)
                 cGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAgggg                                                                                                                                     >  3:24314/1‑149 (MQ=255)
                   ttCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatattt                                                                                                                                   <  7:121965‑M2/149‑20 (MQ=255)
                              gCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGga                                                                                                                         <  4:24314/148‑1 (MQ=255)
                                cggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcaggtggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGga                                                                                                                         >  6:119266‑M2/113‑146 (MQ=255)
                                cggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgaga                                                                                                                       <  3:77480‑M2/36‑1 (MQ=255)
                                  gccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGgagaga                                                                                                                     >  1:5392‑M2/111‑148 (MQ=255)
                                     aCTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcac                                                                                                                 >  2:55591‑M2/1‑112 (MQ=255)
                                          acatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATg                                                                                                                            >  2:139678‑M2/103‑133 (MQ=255)
                                          acatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATg                                                                                                                            <  1:139678‑M2/31‑1 (MQ=255)
                                               aatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATggg                                                                                                                          <  5:43361‑M2/33‑1 (MQ=255)
                                               aatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATggg                                                                                                                          >  6:43361‑M2/98‑130 (MQ=255)
                                                       gttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTaaa                                                                                                >  1:51516‑M2/90‑148 (MQ=255)
                                                        ccccTGCCGATTCGAGGGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCAGAATTTGATAACGGTAACAAGTTTAGtggagttgccccaatatttcaagacatctgttagcacttaacccattacaagccag                                                                                              <  8:103714‑M2/149‑57 (MQ=255)
                                                         cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttc                                                                                                                                  >  4:47501‑M2/1‑92 (MQ=255)
                                                                gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgca                                                                                      >  3:24936‑M2/1‑85 (MQ=255)
                                                                gATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgca                                                                                      >  8:121965‑M2/1‑85 (MQ=255)
                                                                    tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAg                                                                                  >  8:106858‑M2/77‑149 (MQ=255)
                                                                     gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccaga                                                                                                                              <  4:125199‑M2/104‑25 (MQ=255)
                                                                     gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccaga                                                                                                                              >  3:125199‑M2/1‑80 (MQ=255)
                                                                       tctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGa                                                                                <  2:51516‑M2/75‑1 (MQ=255)
                                                                         taaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATc                                                                                                                                                <  3:79398‑M2/11‑1 (MQ=255)
                                                                         taaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATc                                                                                                                                                >  4:79398‑M2/72‑82 (MQ=255)
                                                                            ttGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                          <  1:85280‑M2/149‑77 (MQ=255)
                                                                             tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                          >  4:94519‑M2/1‑72 (MQ=255)
                                                                                tagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCgg                                                                      >  3:63764‑M2/65‑149 (MQ=255)
                                                                                      aaGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgat                                                                 <  3:66939‑M2/148‑86 (MQ=255)
                                                                                       aGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataa                                                               >  4:66939‑M2/1‑62 (MQ=255)
                                                                                        gCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataac                                                              <  6:138738‑M2/149‑89 (MQ=255)
                                                                                          tGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                              <  4:80839‑M2/131‑73 (MQ=255)
                                                                                          tGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                              >  3:80839‑M2/1‑59 (MQ=255)
                                                                                             aaCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagc                                                         <  1:119261‑M2/149‑94 (MQ=255)
                                                                                              aCCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcg                                                        <  4:6850‑M2/149‑95 (MQ=255)
                                                                                                      tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgc                                                >  2:85280‑M2/1‑47 (MQ=255)
                                                                                                      tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgc                                                <  7:105016‑M2/149‑103 (MQ=255)
                                                                                                      tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttcca                                                                                                                                <  7:21165‑M2/69‑23 (MQ=255)
                                                                                                      tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttcca                                                                                                                                >  8:21165‑M2/1‑47 (MQ=255)
                                                                                                        tCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcg                                               <  4:53974‑M2/148‑104 (MQ=255)
                                                                                                        tCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcg                                               >  3:53974‑M2/1‑45 (MQ=255)
                                                                                                                   ggggtctggagattcagggggccagtctaTTAAGGGCATCTGACGGAGGGGGAAGGGTTGGGAGAGCCAGGCGGCACTTACCGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATCAGGGGATTCTGCGAGAACCAAATCCCCCTg                                   >  8:93270‑M2/30‑149 (MQ=255)
                                                                                                                    ttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                                  >  5:54554‑M2/29‑149 (MQ=255)
                                                                                                                         cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                               <  2:131424‑M2/99‑72 (MQ=255)
                                                                                                                         cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                               >  1:131424‑M2/1‑28 (MQ=255)
                                                                                                                          ggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATgg                            >  2:85039‑M2/23‑149 (MQ=255)
                                                                                                                             aggggataaCGGTAACATGGTTAGtggatgtgcccctatatttccagccatctgttagcacttaacccattacaagcccgctgccgcagatattcccgtggcgtgcgataacccagcgcactatgcggatgccattcgttataatgctc                         >  7:103714‑M2/5‑24 (MQ=255)
                                                                                                                                tgATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgcctttcgttataatgctcgaa                      >  2:119261‑M2/1‑21 (MQ=255)
                                                                                                                                 cagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGa                       <  6:54554‑M2/132‑1 (MQ=255)
                                                                                                                                    aaCGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaa                      <  2:52517‑M2/145‑129 (MQ=255)
                                                                                                                                    aaCGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaa                      >  1:52517‑M2/1‑17 (MQ=255)
                                                                                                                                     aCGGTAACAAGTTTAGtggatttgcccctatatttcccgccatctgtgatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccatgcgttataatgctcgaacgcct                 <  8:91023‑M2/149‑134 (MQ=255)
                                                                                                                                              taTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAgg                                                                                 <  4:49637‑M2/74‑1 (MQ=255)
                                                                                                                                              taTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAgg                                                                                 >  3:49637‑M2/3‑76 (MQ=255)
                                                                                                                                                    gtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttg  <  5:27571‑M2/149‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910942‑911238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.