Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,422	G→A	A543V (GCC→GTC)	poxB ←	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,422	0	G	A	100.0%	100.8 / NA	31	A543V (GCC→GTC)	poxB	pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/17);  total (14/17)

ATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCC  >  NC_000913/909290‑909559                                                                                                                                     |                                                                                                                                          atcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGAt                                                                                                                           >  8:108538/1‑149 (MQ=255)  tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGt                                                                                                                                <  5:31026/143‑1 (MQ=255)  tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGt                                                                                                                                >  6:31026/1‑143 (MQ=255)       gCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACATGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCgg                                                                                                                     <  5:3012/149‑1 (MQ=255)       gCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCgg                                                                                                                     <  4:32684/149‑1 (MQ=255)         aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTg                                                                                                                   >  2:126755/1‑149 (MQ=255)            tCCTTATTATGACGGGAAATGCCACCCTTTTTACATTAGCCAGTTTGTTTTCGCCAGTTCGATAACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGaa                                                                                                                >  7:51927/1‑149 (MQ=255)              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATg                                                                                                              >  2:114514/1‑149 (MQ=255)                            aaaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCttt                                                                                                <  6:30842/149‑1 (MQ=255)                             aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                                                                                               <  8:42602/149‑1 (MQ=255)                               tGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGc                                                                                             <  1:126755/149‑1 (MQ=255)                                gCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGc                                                                                             >  2:36728/1‑148 (MQ=255)                                 ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt                                                                                                               >  5:124005/1‑129 (MQ=255)                                 ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt                                                                                                               <  6:124005/129‑1 (MQ=255)                                             ccTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATcca                                                                               >  5:87648/1‑149 (MQ=255)                                                                gCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                            <  7:108538/149‑1 (MQ=255)                                                                 ccAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATg                                                           <  6:87648/149‑1 (MQ=255)                                                                        gATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAgg                                                    >  3:67990/1‑149 (MQ=255)                                                                                 atcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCa                                           <  8:51927/149‑1 (MQ=255)                                                                                 atcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATACACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCa                                           <  4:67990/149‑1 (MQ=255)                                                                                  tcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATcc                                                                                <  2:131626/111‑1 (MQ=255)                                                                                  tcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATcc                                                                                >  1:131626/1‑111 (MQ=255)                                                                                  tcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAg                                          <  1:114514/149‑1 (MQ=255)                                                                                  tcaCCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAg                                          >  1:75058/1‑149 (MQ=255)                                                                                           ccGCTGATGATTGCGCGCAGCATATACAGGCTGAACCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAATTCTTCTTTGGCGACCACCACATCCACCTACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTtcatc                                 <  8:54886/149‑1 (MQ=255)                                                                                               tgatgaTTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                            >  2:54588/1‑118 (MQ=255)                                                                                               tgatgaTTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGAt                                                            <  1:54588/118‑1 (MQ=255)                                                                                                            cagcaTATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCttttt                <  2:75058/149‑1 (MQ=255)                                                                                                                     cAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGa       >  2:94301/1‑149 (MQ=255)                                                                                                                          tGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATAccc  >  5:33959/1‑149 (MQ=255)                                                                                                                            aaaCCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATAccc  <  1:36728/147‑1 (MQ=255)                                                                                                                                     |                                                                                                                                          ATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCC  >  NC_000913/909290‑909559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑