Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,768,131	A→T	pseudogene (911/945 nt)	rhsJ →	Rhs‑family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,768,131	0	A	T	100.0%	89.7 / NA	27	pseudogene (911/945 nt)	rhsJ	Rhs‑family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/14);  total (13/14)

TAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATG  >  NC_000913/3767991‑3768269                                                                                                                                             |                                                                                                                                           tAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGc                                                                                                                                    <  2:116610/149‑1 (MQ=255)      gTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGa                                                                                                                                >  6:22308/1‑148 (MQ=255)                        cAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGatat                                                                                                                 >  3:70222/1‑145 (MQ=255)                           tGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAg                                                                                                          >  1:3569/1‑149 (MQ=255)                           tGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCAAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATAAGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAg                                                                                                          >  6:102110/1‑149 (MQ=255)                             cTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAgg                                                                                                         <  7:74781/148‑1 (MQ=255)                             cTAACAGCACCAACGAACATTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGTTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAgg                                                                                                         <  7:73406/148‑1 (MQ=255)                              tAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGtt                                                                                                       >  2:10105/1‑149 (MQ=255)                                 cAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTa                                                                                                    <  1:10105/149‑1 (MQ=255)                                   gCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGa                                                                                                  <  8:24454/149‑1 (MQ=255)                                                            aGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGGTGTAGATGTATGAAAGCATGCTTGTTACTAt                                                                         <  7:7937/149‑1 (MQ=255)                                                                   aaTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTa                                                                  >  6:33930/1‑149 (MQ=255)                                                                              caacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTa                                                       >  3:99572/1‑149 (MQ=255)                                                                                 caaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                    >  8:1385/1‑149 (MQ=255)                                                                                        ccTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAAtt                                             <  7:1385/149‑1 (MQ=255)                                                                                             gTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATg                                        <  2:116474/149‑1 (MQ=255)                                                                                                      tAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATATTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGAtg                               >  8:8202/1‑149 (MQ=255)                                                                                                       aaCATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAACCCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCGTGTTACTAGTTTTTTATGTCTCTTTTATTTGTCAATTGCATGGTGCTGAtgt                              >  3:59038/1‑149 (MQ=255)                                                                                                               ggCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                    <  4:68935/119‑1 (MQ=255)                                                                                                               ggCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAttt                                                    >  3:68935/1‑119 (MQ=255)                                                                                                               ggCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaa                      <  5:22308/149‑1 (MQ=255)                                                                                                                 ccATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaa                    <  5:33930/149‑1 (MQ=255)                                                                                                                     cTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaa                 >  7:58063/1‑148 (MQ=255)                                                                                                                                 tttCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATTTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatga    <  5:102110/149‑1 (MQ=255)                                                                                                                                    cTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTTAAAATAAAACAAAACGAAAGTatgatg  <  4:99572/148‑1 (MQ=255)                                                                                                                                       atatGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaa           >  4:90395/1‑136 (MQ=255)                                                                                                                                       atatGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaa           <  3:90395/136‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           TAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATG  >  NC_000913/3767991‑3768269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑