Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)17 (0.490) 16/276 1.2 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 0 (0.000)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)29 (0.850) 24/274 0.5 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 0 (0.000)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

TCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910949‑911238
                                                                                                                                             |                                                                                                                                                    
tCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggattt                                                                                                                                               <  7:123226‑M2/149‑8 (MQ=255)
               agagagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttc                                                                                                                                  <  8:86265‑M2/147‑21 (MQ=255)
                 agagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccaga                                                                                                                              >  4:39573‑M2/1‑125 (MQ=255)
                     tCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtgga                                                                                                                                                  <  8:104896‑M2/125‑5 (MQ=255)
                     tCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtgga                                                                                                                                                  >  7:104896‑M2/1‑121 (MQ=255)
                               taagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAag                                                                                                                  >  7:126383‑M2/107‑147 (MQ=255)
                                  cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGCTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcactta                                                                                                              >  5:132582‑M2/1‑108 (MQ=255)
                                      tcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGTTAGTTCATCTGGCGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACt                                                                                                          >  8:104575‑M2/100‑148 (MQ=255)
                                               cACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagc                                                                                                 >  7:86265‑M2/1‑95 (MQ=255)
                                                  cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaa                                                                                                   >  8:93415‑M2/1‑92 (MQ=255)
                                                  cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaa                                                                                                   <  7:93415‑M2/143‑52 (MQ=255)
                                                  cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctata                                                                                                                                      <  6:6743‑M2/108‑17 (MQ=255)
                                                  cccTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctata                                                                                                                                      >  5:6743‑M2/1‑92 (MQ=255)
                                                    tccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGcc                                                                                           >  1:59417‑M2/86‑149 (MQ=255)
                                                     ccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGccc                                                                                          >  5:65295‑M2/85‑149 (MQ=255)
                                                                        ctagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGaaa                                                                                                                   >  1:18694‑M2/66‑105 (MQ=255)
                                                                        ctagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGaaa                                                                                                                   <  2:18694‑M2/40‑1 (MQ=255)
                                                                          gATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgaccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgag                                                                     <  1:26996‑M2/149‑82 (MQ=255)
                                                                              ttaagacaatcacttatttcagtgatatagggtgtctggagattcagggggccagtcaaTTTAGCTCATCTGACGGCGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                                   <  8:113523‑M2/88‑1 (MQ=255)
                                                                                       tcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGAt                                                          <  2:59417‑M2/97‑1 (MQ=255)
                                                                                          gtttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattccc                                                                             <  2:169435‑M2/125‑74 (MQ=255)
                                                                                                   aTGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggat                                            <  1:117130‑M2/149‑107 (MQ=255)
                                                                                                    tgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCaaa                                           >  6:109125‑M2/38‑149 (MQ=255)
                                                                                                      gTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaccccattacaagcccgctgacgcagatattcccgtggcgagcgataacccagcgcactatgcggatgc                                          <  4:5253‑M2/148‑109 (MQ=255)
                                                                                                      gTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgc                                          <  4:7116‑M2/148‑109 (MQ=255)
                                                                                                          tcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttacagacatctgttatcacttaacccattacaagaccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattc                                     <  3:39368‑M2/149‑114 (MQ=255)
                                                                                                           ggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGAGAGAACCAAATCCCCCt                                    >  5:15229‑M2/31‑149 (MQ=255)
                                                                                                             aTCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgtt                                  >  5:140175‑M2/1‑33 (MQ=255)
                                                                                                             aTCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgt                                   <  6:140175‑M2/148‑116 (MQ=255)
                                                                                                                tctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa                               >  5:85321‑M2/26‑149 (MQ=255)
                                                                                                                  cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataat                             <  5:26072‑M2/149‑122 (MQ=255)
                                                                                                                   tGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatg                            <  5:138186‑M2/149‑123 (MQ=255)
                                                                                                                      gttgcagggggccagtctaTTTATTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAg                         <  6:15229‑M2/130‑1 (MQ=255)
                                                                                                                      gattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAg                         <  6:85321‑M2/130‑1 (MQ=255)
                                                                                                                           cgggggccaggctcTTTAGTTCATCTGACGGAGGCGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCa                    <  6:65295‑M2/135‑1 (MQ=255)
                                                                                                                              gggccaggctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGc                 <  8:126383‑M2/138‑1 (MQ=255)
                                                                                                                              aCGGGAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcct                 >  8:123226‑M2/1‑16 (MQ=255)
                                                                                                                               cGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctc                >  2:26996‑M2/1‑15 (MQ=255)
                                                                                                                               cGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctc                >  3:46740‑M2/1‑15 (MQ=255)
                                                                                                                                ggTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctct               >  2:117130‑M2/1‑14 (MQ=255)
                                                                                                                                  tAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataa                                                               >  3:62133‑M2/1‑12 (MQ=255)
                                                                                                                                  tAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataa                                                               <  4:62133‑M2/99‑88 (MQ=255)
                                                                                                                                             gtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttctttg  >  3:3870‑M2/1‑1 (MQ=255)
                                                                                                                                             gtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggttcttt   >  6:26072‑M2/1‑1 (MQ=255)
                                                                                                                                             gTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGAt                                                                                       <  2:27251/64‑1 (MQ=255)
                                                                                                                                             gTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGAt                                                                                       >  1:27251/1‑64 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTC  >  NC_000913/910949‑911238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.