| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,739,764 | 0 | G | T | 50.0% | ‑6.4 / 22.5 | 16 | A12E (GCG→GAG) | ydhB | LysR family putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base T (5/3); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATAC > NC_000913/1739620‑1739910 | gcGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACTCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCTCATc < 3:112319/149‑1 (MQ=255) cacaTCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCTCATCAACAACTTc > 5:136534/1‑147 (MQ=255) caTCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGt < 3:117732/149‑1 (MQ=255) caTCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGt < 4:1685/149‑1 (MQ=255) aaGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCTCATCAACAACTTCGAGTGAATATTCTGACCACATAGt < 6:136534/149‑1 (MQ=255) ccaCTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAAttttt < 6:86856/149‑1 (MQ=255) gCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCTCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCaaa > 5:61800/1‑148 (MQ=255) aaCGCGATGCAGCTCATGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAat > 5:62464/1‑124 (MQ=255) aaCGCGATGCAGCTCATGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAat < 6:62464/124‑1 (MQ=255) aaCTACCATTACGCGCTACCTCATCAACACCTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAACCCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAAtt > 4:83505/1‑147 (MQ=255) aaCTACCATTACGCGCTACCTCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTga < 6:61800/149‑1 (MQ=255) aaCTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAAtt > 1:68715/1‑147 (MQ=255) ccATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGAt > 2:5237/1‑149 (MQ=255) cATTACGCGCTACCTCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGAtt > 3:99929/1‑149 (MQ=255) tACCTCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGACTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAata > 6:140015/1‑148 (MQ=255) ccGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATAGAGAGATTTAGAGAATATAc < 3:60852/149‑1 (MQ=255) | GCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAACGCGATGCAGCTCCTGTGCCGCAGCGCTAAAACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATAC > NC_000913/1739620‑1739910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |