Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,290,9590CA50.0% 1.8 / 37.3 26L716I (CTC→ATC) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (7/6);  new base A (7/6);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAA  >  NC_000913/3290818‑3291104
                                                                                                                                             |                                                                                                                                                 
tcGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCg                                                                                                                                              <  6:84622/147‑1 (MQ=255)
           gggCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACg                                                                                                                                  >  6:119104/1‑148 (MQ=255)
                                     cTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGaa                                                                                                       <  5:119104/149‑1 (MQ=255)
                                      tAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGaa                                                                                                       >  2:103012/1‑148 (MQ=255)
                                       aaCGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAACCCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGaac                                                                                                      >  7:91151/1‑148 (MQ=255)
                                                           gATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTgtgg                                                                                    >  1:165969/1‑146 (MQ=255)
                                                           gATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTgtgg                                                                                    <  2:165969/146‑1 (MQ=255)
                                                                    cTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCCGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACagag                                                                        <  6:118102/149‑1 (MQ=255)
                                                                                  cTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACagag                                                                        >  7:69507/1‑135 (MQ=255)
                                                                                  cTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACagag                                                                        <  8:69507/135‑1 (MQ=255)
                                                                                  cTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAgcg                                                          >  4:22902/1‑149 (MQ=255)
                                                                                   tACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTgtggt                                                                                   <  2:159762/123‑1 (MQ=255)
                                                                                   tACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTgtggt                                                                                   >  1:159762/1‑123 (MQ=255)
                                                                                   tACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAgcgg                                                         >  5:18721/1‑149 (MQ=255)
                                                                                   tACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGt                                                                      >  2:153034/1‑136 (MQ=255)
                                                                                   tACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGt                                                                      <  1:153034/136‑1 (MQ=255)
                                                                                            ttttGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTc                                                 >  5:114740/1‑148 (MQ=255)
                                                                                                        aGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCg                                    <  3:22902/149‑1 (MQ=255)
                                                                                                            gTTCCTTATCTCACAACTTATCAGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTa                                >  2:125329/1‑149 (MQ=255)
                                                                                                                            cTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACa                <  6:18721/149‑1 (MQ=255)
                                                                                                                                tCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTc                                                 >  8:98450/1‑112 (MQ=255)
                                                                                                                                  aGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGt                                  <  7:25517/125‑1 (MQ=255)
                                                                                                                                  aGGAAAACCGTATCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGt                                  >  8:25517/1‑125 (MQ=255)
                                                                                                                                    gAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCg                                    <  6:153057/121‑1 (MQ=255)
                                                                                                                                    gAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATAGCg                                    >  5:153057/1‑121 (MQ=255)
                                                                                                                                          cGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCaa  <  1:103012/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
TCGCAATATCTGGGCAACGCTTTTGCGCTTATCGATGCTAACGGAGCATCTGGCGTGAGGATACAAAACTATCCGGGGATTGCTACCGATCCTTTTGGCTATGCAGTGGTTCCTTATCTCACAACTTATCAGGAAAACCGTCTCTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAA  >  NC_000913/3290818‑3291104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: