Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,287	C→A	Y473* (TAC→TAA)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,287	0	C	A	100.0%	147.0 / NA	42	Y473* (TAC→TAA)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/22);  total (20/22)

TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGT  >  NC_000913/1159142‑1159429                                                                                                                                                  |                                                                                                                                               tGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGTCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTACATCCGATAACCTGAAAACCGAGATGGATGAGTAAATc                                                                                                                                             >  3:36807/1‑149 (MQ=255)   cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATcc                                                                                                                                            <  6:64215/148‑1 (MQ=255)      ggcAGCGTTGCTGATGTGTCTAAAGTGTATCAGGCCTGCCTGAAGATCCTGGGCGCAGCGGGCGTAGTGGGTGCTGGTTCTGGTGTTCAGGCGATTTTCGTTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCAGTaa                                                                                                                                        <  4:36807/147‑1 (MQ=255)                 tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                             >  8:32806/1‑149 (MQ=255)                 tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                             >  3:110540/1‑149 (MQ=255)                            aaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGtt                                                                                                                    <  1:38979/147‑1 (MQ=255)                              gTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                                 >  1:112918/1‑148 (MQ=255)                                 gATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                             <  4:1971/149‑1 (MQ=255)                                      ggccggccTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAg                                                                                                        <  4:110540/149‑1 (MQ=255)                                            ccTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATTGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGcc                                                                                                  <  8:91700/149‑1 (MQ=255)                                                      cttggCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc                                                                                        <  2:141118/146‑1 (MQ=255)                                                          gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                                    <  7:32806/149‑2 (MQ=255)                                                          gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtta                                                                                    <  3:56307/149‑2 (MQ=255)                                                                     gTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                         <  2:112918/149‑1 (MQ=255)                                                                           gTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                                  >  3:113062/1‑102 (MQ=255)                                                                           gTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                                  <  4:113062/102‑1 (MQ=255)                                                                                tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                             >  8:38679/1‑134 (MQ=255)                                                                                      tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                                                                                                           <  6:110212/98‑1 (MQ=255)                                                                                      tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACt                                                                                                           >  5:110212/1‑98 (MQ=255)                                                                                              aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                 >  2:103646/1‑148 (MQ=255)                                                                                              aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                 >  6:10891/1‑148 (MQ=255)                                                                                               ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               >  5:88498/1‑149 (MQ=255)                                                                                                gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAAt                                              >  1:170452/1‑149 (MQ=255)                                                                                                gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAAt                                              >  8:79204/1‑149 (MQ=255)                                                                                                             cTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTTAAATTAcg                                  <  2:170452/148‑1 (MQ=255)                                                                                                              tAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCatc                                                                                               >  2:11090/1‑84 (MQ=255)                                                                                                                 aTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                             >  5:4632/1‑117 (MQ=255)                                                                                                                 aTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTg                                                             <  6:4632/117‑1 (MQ=255)                                                                                                                     gATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa                                                                <  8:127172/110‑1 (MQ=255)                                                                                                                     gATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGcc                         <  6:88498/149‑1 (MQ=255)                                                                                                                      aTAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               <  5:145250/126‑1 (MQ=255)                                                                                                                      aTAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                               >  6:145250/1‑126 (MQ=255)                                                                                                                          ccTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGAAATGCTGAAATTACGCGGTGTGCCGTAGa                    <  8:102868/149‑1 (MQ=255)                                                                                                                           cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTa                                                                       <  2:102665/97‑1 (MQ=255)                                                                                                                            tGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                   >  3:134975/1‑148 (MQ=255)                                                                                                                             gAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg                                  <  4:107612/132‑1 (MQ=255)                                                                                                                             gAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg                                  >  3:107612/1‑132 (MQ=255)                                                                                                                                  ccGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGaa            <  4:134975/149‑1 (MQ=255)                                                                                                                                    gagaTGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAcc          >  5:49645/1‑149 (MQ=255)                                                                                                                                            tgAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGt  >  4:61189/1‑149 (MQ=255)                                                                                                                                             gAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                          >  6:112757/1‑60 (MQ=255)                                                                                                                                             gAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                          <  5:112757/60‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               TGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGT  >  NC_000913/1159142‑1159429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑