Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,529	T→A	K346* (AAA→TAA)	mlc ←	glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,529	0	T	A	100.0%	99.2 / NA	29	K346* (AAA→TAA)	mlc	glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/14);  total (15/14)

TCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC  >  NC_000913/1667385‑1667645                                                                                                                                                 |                                                                                                                     tCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACt                                                                                                                   >  4:14002/1‑148 (MQ=255)     gTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACg                                                                                                              >  3:58118/1‑149 (MQ=255)               aGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAAt                                                                                                    <  7:81002/149‑1 (MQ=255)                           gCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTtg                                                                                        >  8:24668/1‑149 (MQ=255)                                     ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCa                                                                                                  <  1:16200/129‑1 (MQ=255)                                     ccTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCa                                                                                                  >  2:16200/1‑129 (MQ=255)                                             gAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCAc                                                                      <  6:37188/149‑1 (MQ=255)                                               aaaCTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCa                                                                    <  7:24668/149‑1 (MQ=255)                                                      gTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                             <  3:14002/149‑1 (MQ=255)                                                         ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGc                                                                                                        <  6:103036/103‑1 (MQ=255)                                                         ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGc                                                                                                        >  5:103036/1‑103 (MQ=255)                                                         ctctCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCaa                                                           <  4:58118/148‑1 (MQ=255)                                                            tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                             <  4:110616/143‑1 (MQ=255)                                                            tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGc                                                             >  3:110616/1‑143 (MQ=255)                                                            tcAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAAt                                                       >  6:152947/1‑149 (MQ=255)                                                                cGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATgcgc                                                   <  5:152947/149‑1 (MQ=255)                                                                            gACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATgcgcg                                       >  6:61701/1‑149 (MQ=255)                                                                                 taCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                 >  2:123516/1‑134 (MQ=255)                                                                                 taCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGccc                                                 <  1:123516/134‑1 (MQ=255)                                                                                 taCGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCAc                                  >  8:95679/1‑149 (MQ=255)                                                                                             gCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTAAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAatgat                      <  8:38070/149‑1 (MQ=255)                                                                                               ctgctgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGt                    >  2:12735/1‑149 (MQ=255)                                                                                                   tgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                              <  2:126103/135‑1 (MQ=255)                                                                                                   tgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCg                              >  1:126103/1‑135 (MQ=255)                                                                                                   tgACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCttt                >  6:17431/1‑149 (MQ=255)                                                                                                     aCGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCtttt               <  1:12735/148‑1 (MQ=255)                                                                                                         aTGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAg          <  5:17431/149‑1 (MQ=255)                                                                                                               tCTGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAg          >  3:38559/1‑143 (MQ=255)                                                                                                                 tGAGATGACCGGGAAGAGGATATCTGCCGCTTAACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATcgc  >  5:112895/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                     TCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGCCTGCTGACGGATGCTGTCTGAGATGACCGGGAAGAGGATATCTGCCGCTTTACTTAACGGTGAGCCAATCAGTATTTTTTGTGGGTTAAATAAATTCACCATGATGGCAAGAATGCGCCCGACATGCGCGCCCACCCCGGTAATGATGTCTTTTGCCAGTAGATCGC  >  NC_000913/1667385‑1667645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑