Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,721,243	G→A	G431R (GGA→AGA)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,243	0	G	A	100.0%	152.3 / NA	43	G431R (GGA→AGA)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/20);  total (23/20)

AGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGG  >  NC_000913/2721104‑2721384                                                                                                                                            |                                                                                                                                              aGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCa                                                                                                                                      <  8:111473/149‑1 (MQ=255)                  tAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTAt                                                                                                                            <  4:65918/142‑1 (MQ=255)                  tAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTAt                                                                                                                            >  3:65918/1‑142 (MQ=255)                                 gcagcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCggtg                                                                                                       >  5:127210/1‑146 (MQ=255)                                     caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTAAGCGAAGCCCGGCTGCCGACCTACCGTACCCCGGAAAGAACCAt                                                                                                                                        >  6:186223/1‑111 (MQ=255)                                     caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCATCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCGGACCTACCGTACCCCGGAAAGAACCAt                                                                                                                                        <  5:186223/111‑1 (MQ=255)                                                     cTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGa                                                                                  <  5:6489/149‑1 (MQ=255)                                                          gAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTa                                                                                                                             <  6:169470/101‑1 (MQ=255)                                                          gAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTa                                                                                                                             >  5:169470/1‑101 (MQ=255)                                                                gTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGt                                                                       >  5:116036/1‑149 (MQ=255)                                                                           agatctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAAt                                                            <  5:4468/145‑1 (MQ=255)                                                                            ctcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGc                                                                         >  5:4026/1‑135 (MQ=255)                                                                            ctcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGc                                                                         <  6:4026/135‑1 (MQ=255)                                                                            ctcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATc                                                           >  4:155113/1‑149 (MQ=255)                                                                             tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                          >  1:44277/1‑149 (MQ=255)                                                                             tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                          >  7:71269/1‑149 (MQ=255)                                                                             tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTGTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                          >  4:67381/1‑149 (MQ=255)                                                                               ctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGTGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                          <  6:127210/147‑1 (MQ=255)                                                                                tcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTgcc                                                       >  7:10561/1‑147 (MQ=255)                                                                                  gCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAAt                                                            >  6:4468/1‑142 (MQ=255)                                                                                    aaGAGGCACGACGTTTATTCGGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCatat                                                                                                                      >  6:28733/1‑82 (MQ=255)                                                                                    aaGAGGCACGACGTTTATTCGGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCatat                                                                                                                      <  5:28733/82‑1 (MQ=255)                                                                                      gagGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCaa                                                 <  6:116036/149‑1 (MQ=255)                                                                                        ggCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATa                                               <  2:44277/149‑1 (MQ=255)                                                                                          cACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATAcc                                             >  3:60714/1‑149 (MQ=255)                                                                                            cgacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGc                                           <  3:155113/149‑1 (MQ=255)                                                                                             gacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTcc                                                   <  2:69236/140‑1 (MQ=255)                                                                                             gacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTcc                                                   >  1:69236/1‑140 (MQ=255)                                                                                                    aTTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAgcg                                    >  4:54783/1‑148 (MQ=255)                                                                                                      tCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                  <  7:75527/68‑1 (MQ=255)                                                                                                      tCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                  >  8:75527/1‑68 (MQ=255)                                                                                                       cAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTCTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAt                                 <  3:67381/148‑1 (MQ=255)                                                                                                        aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATc                                                           <  2:147769/121‑1 (MQ=255)                                                                                                        aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATc                                                           >  1:147769/1‑121 (MQ=255)                                                                                                            aaGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTg                           >  4:24069/1‑149 (MQ=255)                                                                                                              gCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGtt                         <  3:54783/149‑1 (MQ=255)                                                                                                                   gCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAg                                                                <  6:124834/105‑1 (MQ=255)                                                                                                                        cGACCTACCGTACCCCGGAAAGAACCGTCACTGCTTTTATGCATAtggtg                                                                                                                  >  6:128568/1‑50 (MQ=255)                                                                                                                        cGACCTACCGTACCCCGGAAAGAACCGTCACTGCTTTTATGCATAtggtg                                                                                                                  <  5:128568/50‑1 (MQ=255)                                                                                                                                cGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCg        >  8:155421/1‑148 (MQ=255)                                                                                                                                     cccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACa                   >  4:164342/1‑132 (MQ=255)                                                                                                                                     cccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACa                   <  3:164342/132‑1 (MQ=255)                                                                                                                                     cccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAgggg  >  5:119727/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                              AGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGG  >  NC_000913/2721104‑2721384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑