Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 911,086 IS2 (–) +5 bp intergenic (‑37/+92) poxB ← / ← hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)19 (0.620) 18/284 0.7 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 911086 = 0 (0.000)intergenic (‑37/+96) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent
* ? NC_000913 = 382590NA (NA)20 (0.650) 18/282 0.7 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 911090 0 (0.000)intergenic (‑41/+92) poxB/hcr pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent

GACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGT  >  NC_000913/910944‑911231
                                                                                                                                              |                                                                                                                                                 
gACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtg                                                                                                                                             >  3:41287‑M2/1‑147 (MQ=255)
 aCTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTACATCTCCTGAATGTGATAACGGTAACAAGTTTAGtg                                                                                                                                             <  3:114471‑M2/148‑3 (MQ=255)
       gtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGa                                                                                                                                      >  2:25842‑M2/136‑149 (MQ=255)
       aCTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgc                                                                                                                                      >  1:139638‑M2/1‑140 (MQ=255)
                cgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAgggg                                                                                                                              >  6:75972‑M2/127‑148 (MQ=255)
                    agagagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttc                                                                                                                           <  2:110333‑M2/147‑21 (MQ=255)
                      agagTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccaga                                                                                                                       >  7:109567‑M2/1‑125 (MQ=255)
                       gtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCAt                                                                                                                                          <  6:109328‑M2/10‑1 (MQ=255)
                       gtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCAt                                                                                                                                          >  5:109328‑M2/120‑129 (MQ=255)
                        tttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtcgggagattcagggggccagtctaTTTAGTTCATCTGACGGCGGGGGAAGGGATg                                                                                                                     <  6:111046‑M2/31‑1 (MQ=255)
                                  gACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatca                                                                                                           >  2:26696‑M2/1‑113 (MQ=255)
                                  gACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTCTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatca                                                                                                           <  1:45650‑M2/149‑37 (MQ=255)
                                   aCTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcac                                                                                                          >  2:91946‑M2/1‑112 (MQ=255)
                                       cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaa                                                                                                      >  2:133855‑M2/1‑108 (MQ=255)
                                       cccAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaa                                                                                                      >  1:110333‑M2/1‑108 (MQ=255)
                                          atcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCAc                                                                                                    >  3:81595‑M2/101‑148 (MQ=255)
                                          atcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattgggtgtctggagctgcagggggccagtctaCTTAGTTCATCTGACGGCGGGGGAAGGGATGGGAGAGAAAGGAGGCACt                                                                                                   <  2:103608‑M2/49‑1 (MQ=255)
                                                                 ctaggctaaaaactagtattaagactatcacttatttaagtgatattgggtgtctggagagtcagggggccactctaTTTAGTTCATCTGACGGCGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGaaa                                                                            <  6:42437‑M2/72‑1 (MQ=255)
                                                                   gAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatat                                                                          <  1:127721‑M2/149‑70 (MQ=255)
                                                                    gtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAgg                                                                          >  7:96989‑M2/75‑148 (MQ=255)
                                                                       taaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAat                                                                       >  6:89999‑M2/72‑148 (MQ=255)
                                                                        aaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAatat                                                                     >  5:106716‑M2/71‑149 (MQ=255)
                                                                               gATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgag                                                              <  1:126635‑M2/149‑82 (MQ=255)
                                                                                   ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCCCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGcag                                                           >  5:124859‑M2/60‑147 (MQ=255)
                                                                                   ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa                                                            >  3:92495‑M2/60‑147 (MQ=255)
                                                                                           aaCCGTTTGTTTCATGGTTCTCCATCTCCTTAATCTGATAACGGTAACAAGTTTAGaggatttgaccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacacagc                                                  <  5:109581‑M2/149‑94 (MQ=255)
                                                                                             ccGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcg                                                 <  1:120605‑M2/148‑95 (MQ=255)
                                                                                                      aaggtatattggttgtctggagattcagggggccagtctaTTAAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAAc                                        <  6:106716‑M2/108‑1 (MQ=255)
                                                                                                          ggTTCTCAATCTCCTGAATGTGATAACGGTATCAAGTTTAGtggatttgcccctatatttccagacatctgttatcagttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatg                                    >  3:13637‑M2/1‑41 (MQ=255)
                                                                                                                  ttgtctggagattcagggggccagtctaTTTAGTTCATCTGCCGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGc                           <  5:75972‑M2/121‑1 (MQ=255)
                                                                                                                     gctggagcttcagggggccagtctaGTTAGGTCATCTGACGGAGGGGGAAGGGATGGGAGAGCAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGa                        <  6:54498‑M2/124‑1 (MQ=255)
                                                                                                                       cTGAATGTGATAACGGTAACAAGTTTAGttgatttgcacctatatttccagccatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttata                        >  7:65407‑M2/1‑28 (MQ=255)
                                                                                                                       cTGAATGTGATAACGGTAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataat                      <  2:13547‑M2/149‑122 (MQ=255)
                                                                                                                          cgattcagggggcccgtctcTTTACTTCCTCGGCCGGCGGGGGACGGGAGGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCa                   <  6:124859‑M2/129‑1 (MQ=255)
                                                                                                                                 gggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATTGCAGGAGCAg            >  7:109847‑M2/14‑149 (MQ=255)
                                                                                                                                 gggggccagtctaTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAg            <  4:81595‑M2/136‑1 (MQ=255)
                                                                                                                                       tAACAAGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctg       <  6:1377‑M2/148‑137 (MQ=255)
                                                                                                                                           aaGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaagg  <  5:44440‑M2/149‑142 (MQ=255)
                                                                                                                                           aaGTTTAGtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgtcataatgctcgaacgcctctgcaagg  <  2:90086‑M2/149‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GACTGTCACTAAGACCGTTCAGAGAGTCGCCTGTGACTCCCCAGATGCGTTTCACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGAAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGT  >  NC_000913/910944‑911231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.