Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	123,061	C→T	R15R (CGC→CGT)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	123,061	0	C	T	100.0%	11.9 / NA	5	R15R (CGC→CGT)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)

CGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGA  >  NC_000913/122915‑123081                                                                                                                                                   |                     cgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATTGAAACTCGTGa                    <  2:65751/149‑1 (MQ=255)          aaCGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTc             >  2:138683/1‑147 (MQ=255)                   ccTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCggtgta  >  8:43330/1‑144 (MQ=255)                                                                                             ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCggt     >  1:98297/1‑70 (MQ=255)                                                                                             ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGTGACTGGCTCCAGGCggt     <  2:98297/72‑3 (MQ=255)                                                                                                                                                   |                     CGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGA  >  NC_000913/122915‑123081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑