Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,947,884 Δ1 bp 5.9% coding (268/741 nt) yebC ← UPF0082 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,947,8840A. 5.9% 219.3 / 6.7 68coding (268/741 nt)yebCUPF0082 family protein
Reads supporting (aligned to +/- strand):  ref base A (29/34);  new base . (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCC  >  NC_000913/1947739‑1948023
                                                                                                                                                 |                                                                                                                                           
ccTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAAcc                                                                                                                                          <  7:305242/149‑1 (MQ=255)
    ttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCtt                                                                                                                                        <  3:240132/147‑1 (MQ=255)
    ttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTc                                                                                                                                       <  2:333540/148‑1 (MQ=255)
     tttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCg                                                                                                                                      <  3:273598/148‑1 (MQ=255)
         cTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAga                                                                                                                                  <  2:176114/148‑1 (MQ=255)
            aaCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCTCATTTGCTAAATGGATGACGAACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGACGTGCCGCCAGGAACGTAACCTTCGTAgatga                                                                                                                               <  4:86397/148‑1 (MQ=255)
                 aTAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGttt                                                                                                                         >  8:6172/1‑149 (MQ=255)
                    ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCa                                                                                                                      >  6:83452/1‑149 (MQ=255)
                    ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGT‑ACCTTCCTAGAAGAATGTTTGCAt                                                                                                                     >  3:42245/1‑149 (MQ=255)
                         cGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGttt                                                                                                                 <  4:265290/149‑1 (MQ=255)
                         aggAACCATCAGTACCGCGGGTACCGCCACATTTTCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCCTGATTGCCGGGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGttt                                                                                                                 <  8:361660/148‑1 (MQ=255)
                            aaCCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGca                                                                                                              <  4:205368/149‑1 (MQ=255)
                                 tCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGCTAGCCGTGCCGCCAGGACCGTAACCTTc                                                                                                                                       >  4:67153/1‑119 (MQ=255)
                                 tCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatc                                                                                                         <  5:240455/149‑1 (MQ=255)
                                 tCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGGCAGACAGACATTCAATCATGATTGCCGGGCCGCCAGGACCGTAACCTTc                                                                                                                                       <  3:67153/119‑1 (MQ=255)
                                    gTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCatcatcatc                                                                                                      <  5:140308/149‑1 (MQ=255)
                                    gTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCatcatcatc                                                                                                      <  5:140305/149‑1 (MQ=255)
                                         gAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATg                                                                                                                    <  7:184765/130‑1 (MQ=255)
                                         gAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATg                                                                                                                    >  8:184765/1‑130 (MQ=255)
                                          aGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGcc                                                                                                >  2:351766/1‑149 (MQ=255)
                                            gTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCACCGGTACGGTTGCGGTTGTCAGACGGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCca                                                                                              >  3:304219/1‑149 (MQ=255)
                                                 cGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCAcacca                                                                                         >  1:40059/1‑149 (MQ=255)
                                                           gCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCAcaccac                                                                                        <  2:257335/140‑1 (MQ=255)
                                                           gCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCAcaccac                                                                                        >  1:257335/1‑140 (MQ=255)
                                                           gCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTg                                                                               >  4:214669/1‑149 (MQ=255)
                                                             tAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgcg                                                                             >  2:122418/1‑149 (MQ=255)
                                                             tAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCATAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgcg                                                                             >  8:322339/1‑149 (MQ=255)
                                                               aaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAg                                                                                                                                   <  4:150514/93‑1 (MQ=255)
                                                               aaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgcg                                                                             <  7:18150/147‑1 (MQ=255)
                                                               aaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTgcg                                                                             <  6:200685/147‑1 (MQ=255)
                                                                        cGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTGACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgt                                                                  <  4:78283/149‑1 (MQ=255)
                                                                         gCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgtg                                                                 >  1:181135/1‑149 (MQ=255)
                                                                           aCTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgtgt                                                                >  6:158702/1‑148 (MQ=255)
                                                                           aCTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgtgt                                                                >  6:338209/1‑148 (MQ=255)
                                                                             ttCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTcc                                                              >  2:319739/1‑147 (MQ=255)
                                                                             ttCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCAc                                                             <  2:40059/149‑1 (MQ=255)
                                                                                   gacggtacggtTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCCCGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCa                                                       >  2:35325/2‑149 (MQ=255)
                                                                                     cggtacggtTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCATAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtg                                                     <  7:322339/149‑1 (MQ=255)
                                                                                      ggtacggtTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgt                                                    <  5:83452/149‑1 (MQ=255)
                                                                                       gtacggtTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgtt                                                   <  6:372623/149‑1 (MQ=255)
                                                                                       gtacggtTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgtt                                                   >  5:372623/1‑149 (MQ=255)
                                                                                         acggtTGGGGTTGTCAGACAGAAATTCAATCATCTTTGCCTGGCCGCCAGGACCGT‑ACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgttgt                                                 <  4:42245/148‑1 (MQ=255)
                                                                                           ggttgcggttgTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTa                                               <  4:154198/149‑1 (MQ=255)
                                                                                           ggttgcggaagTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGAGCAATTGCGCGGTTCAGTGTGTCACTGGTCATGATGTTa                                               >  2:365714/1‑149 (MQ=255)
                                                                                                 ggttgTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGt                                         >  1:144074/1‑149 (MQ=255)
                                                                                                  gttgTCAGACAGACATTCAATCATGATTGCCGTGGCGCCAGGACCGT‑ACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGc                                       >  6:50188/1‑149 (MQ=255)
                                                                                                      tCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCTTGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCttt                                    <  7:6172/149‑1 (MQ=255)
                                                                                                      tCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCttt                                    <  3:214669/149‑1 (MQ=255)
                                                                                                         gacagacaTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCTCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATc                                 <  1:351766/149‑1 (MQ=255)
                                                                                                          acagacaTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATc                                 >  5:329418/1‑148 (MQ=255)
                                                                                                              acaTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCAccg                            >  6:338429/1‑149 (MQ=255)
                                                                                                                aTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAg                                              <  1:350024/129‑1 (MQ=255)
                                                                                                                aTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAg                                              >  2:350024/1‑129 (MQ=255)
                                                                                                                  tCAATCATGATTGCCGTGCCGCCAGTACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGGATCATCATCACCGCCCACACCACGTGCATTTGCGCGGTTCAGTGTGTCACGGGTGATGTTGTTAGACAGTGCttt                                    <  1:207034/137‑1 (MQ=255)
                                                                                                                  tCAATAATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGAAATTGCGCGCTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGAttt                                    >  2:207034/1‑137 (MQ=255)
                                                                                                                    aaTCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgt                                                    >  6:59939/1‑119 (MQ=255)
                                                                                                                    aaTCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgt                                                    <  5:59939/119‑1 (MQ=255)
                                                                                                                    aaTCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCcgcac                      >  4:209146/1‑149 (MQ=255)
                                                                                                                    aaTCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCcgcac                      <  5:338209/149‑1 (MQ=255)
                                                                                                                            ttGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGAcgc              >  5:41005/1‑149 (MQ=255)
                                                                                                                               ccgtgccgCCAGGACCGTAACCTTCGTAGATGCTGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCggg           >  2:291712/1‑149 (MQ=255)
                                                                                                                               ccgtgccgCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCgg            <  2:181135/148‑1 (MQ=255)
                                                                                                                                cgtgccgCCAGGACCGTAACCTTCGTAGATGATGATTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGt          >  8:302131/1‑149 (MQ=255)
                                                                                                                                 gtggcgCCAGGACCGT‑ACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTa        <  5:50188/149‑1 (MQ=255)
                                                                                                                                  tgccgCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTa        <  1:122418/149‑1 (MQ=255)
                                                                                                                                  tgccgCCAGGACCGTAACCTTCGTAGATGATGATTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTa        <  7:302131/149‑1 (MQ=255)
                                                                                                                                      gccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGt    >  2:319766/1‑149 (MQ=255)
                                                                                                                                        cAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTcc  <  5:158702/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCC  >  NC_000913/1947739‑1948023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑