Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 145,596 G→A 5.0% V172V (GTG→GTA yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913145,5960GA 5.0% 256.1 / 2.5 79V172V (GTG→GTAyadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (44/31);  new base A (2/2);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CATTTGGGGTACTGGCGGACAATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGC  >  NC_000913/145448‑145743
                                                                                                                                                    |                                                                                                                                                   
aaTTTGGTGTACTGGAGTACAATTTGCGCTACCCGATTTTGCATAAACTGAAATACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTg                                                                                                                                                     <  2:212445/148‑1 (MQ=255)
    tgtggTACTGGCTGACAATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGAACCGTATTGCCGATCGACTGGCCAATATCACCGCACAGGATGCCCAACCCGATAATGGCCTGTCGGTGCTa                                                                                                                                                  <  3:14124/145‑1 (MQ=255)
       ggTACTGGCGGACAATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCt                                                                                                                                              <  8:257100/149‑1 (MQ=255)
                 gACAATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCatat                                                                                                                                    >  7:80442/1‑149 (MQ=255)
                  aCAATTTGCTCTACCCGATTTTGCATAAACTGAAAGAAAGGGTAAATCAAACATGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCata                                                                                                                                     <  5:103749/147‑1 (MQ=255)
                    aaTTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCt                                                                                                                                 <  7:14364/149‑1 (MQ=255)
                      tttGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTgc                                                                                                                               >  4:248820/1‑149 (MQ=255)
                      tttGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTgc                                                                                                                               >  2:32938/1‑149 (MQ=255)
                      tttGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTgc                                                                                                                               <  1:32938/149‑1 (MQ=255)
                      tttGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATACCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTgc                                                                                                                               >  2:20827/1‑149 (MQ=255)
                       ttGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAAAGGCCTGTCGGTGCTAACCTATCACCATATTcagag                                                                                                                              >  3:63336/1‑145 (MQ=255)
                              aCCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGcgacg                                                                                                                         >  6:213164/1‑147 (MQ=255)
                                ccGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACgaaga                                                                                                                     <  5:213164/149‑1 (MQ=255)
                                ccGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACgaag                                                                                                                      >  5:318736/1‑148 (MQ=255)
                                        gCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGcgac                                                                                                                          <  1:44067/136‑1 (MQ=255)
                                        gCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGcgac                                                                                                                          >  2:44067/1‑136 (MQ=255)
                                          aTAAACTGAAAGAAAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGATAACCTAGCACCATATTCTGCGCGACGAAGAAAACACCCGt                                                                                                           >  6:134257/1‑149 (MQ=255)
                                            aaaCTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCACAACCCGATAATGGCCTGTCGGTACTAACCTATCACCATATTCTGCGCGACGAAGAAAACCCCCGttt                                                                                                         >  3:202610/1‑149 (MQ=255)
                                            aaaCTGAAAGACAGGGTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCCACCCGATAATGGCCTTTCGGTACTAACCTATCACCATATTCTGCGCGACGAAGAACCCACCCGttt                                                                                                         >  3:197791/1‑149 (MQ=255)
                                              aCTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTc                                                                                                       <  8:80442/149‑1 (MQ=255)
                                                tGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATc                                                                                                                                           >  7:110156/1‑111 (MQ=255)
                                                tGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATc                                                                                                                                           <  8:110156/111‑1 (MQ=255)
                                                tGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGc                                                                                                     >  7:47427/1‑149 (MQ=255)
                                                tGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGc                                                                                                     >  7:223859/1‑149 (MQ=255)
                                                 gAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGc                                                                                                     >  5:75220/1‑148 (MQ=255)
                                                  aaaGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTc                                                                                                       <  7:26243/145‑1 (MQ=255)
                                                  aaaGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTc                                                                                                       >  8:26243/1‑145 (MQ=255)
                                                     gACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATAc                                                                                                <  6:318736/149‑1 (MQ=255)
                                                           ttAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATAtt                                                                                                                                   <  6:10780/108‑1 (MQ=255)
                                                           ttAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTcgac                                                                                          >  1:365068/1‑149 (MQ=255)
                                                             aaaTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTcgacga                                                                                        >  8:179329/1‑149 (MQ=255)
                                                              aaTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACt                                                                                               <  3:284660/141‑1 (MQ=255)
                                                              aaTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACt                                                                                               >  4:284660/1‑141 (MQ=255)
                                                               aTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCgacgac                                                                                       >  1:73417/1‑148 (MQ=255)
                                                               aTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCgacgac                                                                                       >  1:372228/1‑148 (MQ=255)
                                                               aTCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAAAGGCCTGTCGGTGCTAACCTATAACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGAcc                                                                                      >  5:227746/1‑149 (MQ=255)
                                                                tCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACca                                                                                     >  6:272349/1‑149 (MQ=255)
                                                                  aaaCCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACcaca                                                                                   >  5:358682/1‑149 (MQ=255)
                                                                    cccTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCTTATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATc                                                                                 <  5:322711/148‑1 (MQ=255)
                                                                            tCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCgacgac                                                                                       <  7:52392/135‑1 (MQ=255)
                                                                            tCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCgacgac                                                                                       >  8:52392/1‑135 (MQ=255)
                                                                                       ttGGCGATCGACTGGCATATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAAc                                                              <  6:75220/149‑1 (MQ=255)
                                                                                            gATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGAt                                                         >  2:372084/1‑149 (MQ=255)
                                                                                                gACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGc                                                      <  2:365068/148‑1 (MQ=255)
                                                                                                     gCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCt                                                >  8:82587/1‑149 (MQ=255)
                                                                                                         atatCAGCGCACTGGATGCCCAACCCGATAAAGGCCTGTCGGAGCTAACCTATCACCAAATACTGCGCCACGAAGAAAACACCCGAGTTCGCCATACTTCGACGACCACCTCGCTACGCGCTTTCAAAAACCAGATGGCCTGGCTCCGt                                            >  4:172867/1‑149 (MQ=255)
                                                                                                            tCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCTTATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACTACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt                                            <  3:212065/146‑1 (MQ=255)
                                                                                                            tCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCTTATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGt                                            >  4:212065/1‑146 (MQ=255)
                                                                                                               gcgcACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTAt                                                                                                                                            <  4:168750/47‑1 (MQ=255)
                                                                                                               gcgcACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTAt                                                                                                                                            >  3:168750/1‑47 (MQ=255)
                                                                                                                cgcCCTGGATGCCCAACCCGATAATGGCCTGTCGGTACAATCCTATCACCATATGCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg                                     <  4:202610/149‑1 (MQ=255)
                                                                                                                cgcACTGGATGCCCAACCCGATAATGTCCTGTCGGTACTAACCTATCACCATATGCTGCGCGACGAAGAAAACCCCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg                                     <  4:197791/149‑1 (MQ=255)
                                                                                                                cgcACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACgggg                                     <  3:248820/149‑1 (MQ=255)
                                                                                                                cgcACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAggg                                     <  8:223859/149‑1 (MQ=255)
                                                                                                                  cACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGa                                   <  2:73417/149‑1 (MQ=255)
                                                                                                                     tGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAGACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATAc                                >  7:53820/1‑149 (MQ=255)
                                                                                                                       gATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATAcg                               <  8:223543/148‑1 (MQ=255)
                                                                                                                       gATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATAcg                               >  7:223543/1‑148 (MQ=255)
                                                                                                                         tGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCGGCGTGACAGGGGATACGCGa                            <  4:63336/149‑1 (MQ=255)
                                                                                                                            ccAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGCAAACACCCGTTTTCGCCATACTTCGACGAAAACATCGGTACGAGCTTTCAATAACAAGATGGCATGGCTGCGTGACAGTTGATACGAGacac                         >  2:260533/1‑149 (MQ=255)
                                                                                                                            ccAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGacac                         >  8:283519/1‑149 (MQ=255)
                                                                                                                            ccAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGaca                          >  4:1161/1‑148 (MQ=255)
                                                                                                                             cAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        <  5:272349/149‑1 (MQ=255)
                                                                                                                              aaCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        >  1:147973/1‑148 (MQ=255)
                                                                                                                              aaCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        >  5:338436/1‑148 (MQ=255)
                                                                                                                              aaCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        >  2:216300/1‑148 (MQ=255)
                                                                                                                              aaCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        >  1:381117/1‑148 (MQ=255)
                                                                                                                              aaCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACt                        >  1:256784/1‑148 (MQ=255)
                                                                                                                               aCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAGACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGa                      <  8:53820/149‑1 (MQ=255)
                                                                                                                                   gATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTa                                                                             <  6:354292/90‑1 (MQ=255)
                                                                                                                                   gATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTa                                                                             >  5:354292/1‑90 (MQ=255)
                                                                                                                                   gATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCAt                  >  6:130568/1‑149 (MQ=255)
                                                                                                                                       aTGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGt               <  7:179329/148‑1 (MQ=255)
                                                                                                                                       aTGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTg              >  2:102194/1‑149 (MQ=255)
                                                                                                                                          gCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGcc                                                     <  2:41749/107‑1 (MQ=255)
                                                                                                                                          gCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGcc                                                     >  1:41749/1‑107 (MQ=255)
                                                                                                                                               tCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTg        <  1:20827/147‑1 (MQ=255)
                                                                                                                                                 ggTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGaa     <  1:102194/148‑1 (MQ=255)
                                                                                                                                                  gTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAgg   >  4:9718/1‑149 (MQ=255)
                                                                                                                                                   tGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGc  >  6:37279/1‑149 (MQ=255)
                                                                                                                                                    gCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGc  >  1:50865/1‑148 (MQ=255)
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CATTTGGGGTACTGGCGGACAATTTGCGCTACCCGATTTTGCATAAACTGAAAGACAGGTTAAATCAAACCTGGTATCAGATCCGTATTGGCGATCGACTGGCCTATATCAGCGCACTGGATGCCCAACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGC  >  NC_000913/145448‑145743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: