Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,819,955 C→T 5.3% T420T (ACG→ACA)  chbC ← N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,9550CT 5.3% 301.8 / 3.1 93T420T (ACG→ACA) chbCN,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base C (43/45);  new base T (2/3);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

CATCGTTCCTCTCTTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATGTAGTA  >  NC_000913/1819807‑1820089
                                                                                                                                                    |                                                                                                                                      
cagcGTTCCTCTCTTCTCTTACCGGCACGATTACCCGGACCGGCAGCGATTAAAATTTCAGGGCGGTAGCGATATCTTCTTCGCTCTCTGCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAAc                                                                                                                                        <  6:5900/146‑1 (MQ=255)
 aTCGTTCCTCTCTTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACg                                                                                                                                       <  5:187768/149‑1 (MQ=255)
 aTCGTTCCTCTCTTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACg                                                                                                                                       >  1:253186/1‑149 (MQ=255)
 aTCGTTCCTCTCTTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACg                                                                                                                                       >  1:259506/1‑149 (MQ=255)
        ctctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGAt                                                                                                                                >  7:241499/1‑149 (MQ=255)
        ctctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGAt                                                                                                                                >  4:58829/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGc                                                                                                                                   >  2:291447/1‑145 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGc                                                                                                                                   <  1:291447/145‑1 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  6:237078/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  5:188067/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  3:2144/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  8:118718/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  8:148737/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  5:61015/1‑149 (MQ=255)
         tctctTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTAACAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATg                                                                                                                               >  2:169183/1‑149 (MQ=255)
             ttttCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGCTGcc                                                                                                                             >  4:291338/1‑147 (MQ=255)
                tCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAgg                                                                                                                         <  7:148737/148‑1 (MQ=255)
                 cTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGtt                                                                                                                       >  2:103970/1‑149 (MQ=255)
                 cTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGt                                                                                                                        <  5:237078/148‑1 (MQ=255)
                 cTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGt                                                                                                                        <  4:2144/148‑1 (MQ=255)
                  ttACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGc                                                                                                                                   >  6:293509/1‑136 (MQ=255)
                  ttACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGc                                                                                                                                   <  5:293509/136‑1 (MQ=255)
                    aCCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGa                                                                                                                     <  3:58829/148‑1 (MQ=255)
                     ccGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGaa                                                                                                                    <  2:253186/148‑1 (MQ=255)
                     ccGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGaa                                                                                                                    <  7:336974/148‑1 (MQ=255)
                      cGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAATGTTGCGATGCCAAGGTTGAaga                                                                                                                  <  8:225791/149‑1 (MQ=255)
                      cGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGaa                                                                                                                    >  7:362375/1‑147 (MQ=255)
                      cGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAaga                                                                                                                  <  3:245966/149‑1 (MQ=255)
                       ggCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAagag                                                                                                                 >  7:54655/1‑149 (MQ=255)
                        gCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCTAAGGTTGAAGAGt                                                                                                                >  1:335146/1‑149 (MQ=255)
                          aCGATTACCCTTACCGGCATCGATTAATATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGc                                                                                                              <  1:10466/149‑1 (MQ=255)
                            gATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGa                                                                                                            <  8:362375/149‑1 (MQ=255)
                             aTTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGAc                                                                                                           <  6:188067/149‑1 (MQ=255)
                              ttACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGAcc                                                                                                          <  8:54655/149‑1 (MQ=255)
                                 cccGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCTAAGGTTGAAGAGTGCGACCAg                                                                                                        <  2:335146/148‑1 (MQ=255)
                                  ccGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGc                                                                                                       >  1:193870/1‑148 (MQ=255)
                                       ccGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATgcg                                                                                                 <  4:237659/149‑1 (MQ=255)
                                       ccGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATgc                                                                                                  <  7:377551/148‑1 (MQ=255)
                                       ccGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATgc                                                                                                  <  3:226422/148‑1 (MQ=255)
                                          gCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATgcggc                                                                                               >  3:320218/1‑148 (MQ=255)
                                                    aaaTTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCg                                                                                     <  8:241499/148‑1 (MQ=255)
                                                    aaaTTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGt                                                                                    >  7:135445/1‑149 (MQ=255)
                                                      aTTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTg                                                                                  <  3:291338/149‑1 (MQ=255)
                                                       tttCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGTCAGATAAATTAACGTTGCGCTGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTgg                                                                                 >  6:173892/1‑149 (MQ=255)
                                                           aGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGgtgt                                                                              <  5:370084/148‑1 (MQ=255)
                                                             ggCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTaa                                                                           <  1:103970/149‑1 (MQ=255)
                                                                gTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAaag                                                                         >  3:132628/1‑148 (MQ=255)
                                                                      gATATCTTCTTCGCTCTCTTCTTTAACAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGGGTTAAAGAAGGCTc                                                                  <  1:169183/149‑1 (MQ=255)
                                                                      gATATCTTCTTCGCGCTCTGCTTTATCAATCGCATTTGGGGCTTTGGTAGCCACCACAACAAAGGGCAGATAAAGTACCGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACTCTACCGTTGGTGGTTAAGAAGGCTc                                                                  >  6:270379/1‑149 (MQ=255)
                                                                        tatCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTccc                                                                <  3:206556/149‑1 (MQ=255)
                                                                          tcttctTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAg                                                              >  4:179094/1‑149 (MQ=255)
                                                                             tcttcGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGAcc                                                           >  6:18958/1‑149 (MQ=255)
                                                                               ttcGCTGTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGGCACCACAACAAAGGGCAGTTAAATTTACGTTGCGATGCGCTGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGCGGGCTccct                                                               >  6:300445/1‑142 (MQ=255)
                                                                               ttcGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAATGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCgg                                                         >  6:86071/1‑149 (MQ=255)
                                                                               ttcGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCgg                                                         >  4:273912/1‑149 (MQ=255)
                                                                               ttcGCGCTCTTCATTATCAATCGCATTTTGTGCTTTGTTAGCGACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCa                                                               <  5:300445/143‑1 (MQ=255)
                                                                                 cGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCgg                                                         <  2:193870/147‑1 (MQ=255)
                                                                                  gCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGtt                                                       <  4:132628/148‑1 (MQ=255)
                                                                                  gCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTg                                                      <  6:61015/149‑1 (MQ=255)
                                                                                    tctctTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGgtg                                                                               <  7:207131/122‑1 (MQ=255)
                                                                                    tctctTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGgtg                                                                               >  8:207131/1‑122 (MQ=255)
                                                                                    tctctTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTgg                                                     >  7:301837/1‑148 (MQ=255)
                                                                                      tcttctTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAaga                                                                                                                  <  1:256556/85‑1 (MQ=255)
                                                                                      tcttctTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAaga                                                                                                                  >  2:256556/1‑85 (MQ=255)
                                                                                      tcttctTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCAt                                                  >  7:368677/1‑149 (MQ=255)
                                                                                      tcttctTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCAt                                                  >  7:164941/1‑149 (MQ=255)
                                                                                        ttcttTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCa                                                   <  3:116370/146‑1 (MQ=255)
                                                                                        ttcttTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCa                                                   <  4:152206/146‑1 (MQ=255)
                                                                                        ttcttTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATCCGGCGGCCCTAGCGTAGGTCTTCTAGGAGGCTCCCAGACCGGTTGGCATgg                                                >  6:165185/1‑149 (MQ=255)
                                                                                          cttTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATgg                                                <  4:320218/147‑1 (MQ=255)
                                                                                                 aaTCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCTGTTGGCATGGTCCACGGt                                        <  3:179094/148‑1 (MQ=255)
                                                                                                 aaTCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTg                                       >  2:136869/1‑149 (MQ=255)
                                                                                                   tCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACTCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGc                                      >  3:282656/1‑148 (MQ=255)
                                                                                                   tCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGc                                      >  6:86416/1‑148 (MQ=255)
                                                                                                          ttGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAATGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  5:86071/148‑1 (MQ=255)
                                                                                                          ttGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  8:301837/148‑1 (MQ=255)
                                                                                                          ttGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  3:273912/148‑1 (MQ=255)
                                                                                                          ttGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  2:259506/148‑1 (MQ=255)
                                                                                                          ttGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  8:368677/148‑1 (MQ=255)
                                                                                                          ttGTGCGTTGTTAGCCACCCCACCACAGGGCAGATCAATTCCCTTTGCGAGGCCACGGGTGAAGCGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTACAGCAGGCTCCCCGACCGGTTGGCATGGTCCACGGTGCAATATTg                               <  5:270379/148‑1 (MQ=255)
                                                                                                             tgCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCa                           >  1:168533/1‑149 (MQ=255)
                                                                                                                    ttAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGGTGGCATGGTCCACGGTGCAATATTGGTCACCggagg                    <  8:135445/149‑1 (MQ=255)
                                                                                                                      aGCCACCACAACAAAGGGCAGATAAATTAATGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGTCGACGCTACCGTTGGTGTTAAAGAAGGCTACCAGACCGGTTGGCAAGGTCCACGGTGCAATATTGTTAACCgtatga                   >  7:150582/1‑143 (MQ=255)
                                                                                                                           ccacAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGATTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATg             >  4:209359/1‑149 (MQ=255)
                                                                                                                               aacaaAGTGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGgt                                                                                <  4:125525/78‑1 (MQ=255)
                                                                                                                               aacaaAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGgt                                                                                >  3:125525/1‑78 (MQ=255)
                                                                                                                                 caaAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATg       <  5:18958/149‑1 (MQ=255)
                                                                                                                                 caaAGGGAAGATAAATTAATGTTGAGATGCCAAGGTTGAAGAGTGCGACAAGCAATGCGGCGACGCTAACGTTGGTGTTAAAGAAGGCTCACAGACCGGTTGGCATGGTCCAAGGTGCAATATTGGTCACCGGAGGAATAATGCCCATg       <  8:150582/149‑1 (MQ=255)
                                                                                                                                  aaaGGGCAGAAAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACAAGCAATGCGGCGACGCTACCTTTGGTGTTAAAGAAGGCTACCAGACCGGTTGCCATGGACCACGGTGCAATATTCGTCACCGGAGGAATAAAGCCCATg       >  2:34924/1‑148 (MQ=255)
                                                                                                                                      ggCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATgtagta  <  7:118718/149‑1 (MQ=255)
                                                                                                                                          gATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATgtagt   >  2:18978/1‑144 (MQ=255)
                                                                                                                                          gATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATgtagt   <  1:18978/144‑1 (MQ=255)
                                                                                                                                               aTTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATg       <  3:320351/135‑1 (MQ=255)
                                                                                                                                               aTTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATg       >  4:320351/1‑135 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
CATCGTTCCTCTCTTTTCTTACCGGCACGATTACCCGTACCGGCATCGATTAAAATTTCAGGGCGTTAGCGATATCTTCTTCGCTCTCTTCTTTATCAATCGCATTTTGTGCTTTGTTAGCCACCACAACAAAGGGCAGATAAATTAACGTTGCGATGCCAAGGTTGAAGAGTGCGACCAGCAATGCGGCGACGCTACCGTTGGTGTTAAAGAAGGCTCCCAGACCGGTTGGCATGGTCCACGGTGCAATATTGGTCACCGGAGGAATAATGCCCATGTAGTA  >  NC_000913/1819807‑1820089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑