Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,945,113 T→A 8.2% N82Y (AAT→TAT)  ruvB ← ATP‑dependent DNA helicase, component of RuvABC resolvasome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,945,1130TA 8.2% 137.1 / 3.5 49N82Y (AAT→TAT) ruvBATP‑dependent DNA helicase, component of RuvABC resolvasome
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

ATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGC  >  NC_000913/1944968‑1945255
                                                                                                                                                 |                                                                                                                                              
aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                                                                                                             >  7:134349/1‑149 (MQ=255)
aTTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                                                                                                             <  7:112101/149‑1 (MQ=255)
   gCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGc                                                                                                                                           <  8:164860/148‑1 (MQ=255)
            aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCAtt                                                                                                                                 >  8:206418/1‑149 (MQ=255)
            aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTAAtt                                                                                                                                 >  8:200907/1‑149 (MQ=255)
            aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                                                                                 >  5:23144/1‑149 (MQ=255)
            aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                                                                                 >  5:37302/1‑149 (MQ=255)
            aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCAtt                                                                                                                                 <  5:178330/149‑1 (MQ=255)
             gCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTg                                                                                                                                <  3:211456/149‑1 (MQ=255)
             gCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTg                                                                                                                                <  8:134349/149‑1 (MQ=255)
              cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTgg                                                                                                                               <  3:165480/149‑1 (MQ=255)
               aCTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGc                                                                                                                              >  7:34390/1‑149 (MQ=255)
                          aacTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                                                                                                   >  2:257706/1‑149 (MQ=255)
                           acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATATCATTGGCGACGATGGTGGc                                                                                                                  <  2:166405/149‑1 (MQ=255)
                               gCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGc                                                                                                              <  7:217716/149‑1 (MQ=255)
                                     gACGGTGGATCTCATAAATAAACAGCACGTCATGCGGTTCAAGGTTAGGGAGCCTCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGt                                                                                                         <  5:150137/148‑1 (MQ=255)
                                              tctcATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAAc                                                                                               >  6:220936/1‑149 (MQ=255)
                                                  atcaATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCaa                                                                                                <  2:139364/144‑1 (MQ=255)
                                                  atcaATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCaa                                                                                                >  1:139364/1‑144 (MQ=255)
                                                     aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag                                                                                        <  2:325754/149‑1 (MQ=255)
                                                     aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgga                                                                                         <  6:23144/148‑1 (MQ=255)
                                                     aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgga                                                                                         <  8:159189/148‑1 (MQ=255)
                                                     aaTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCg                                                                                           >  7:159189/1‑146 (MQ=255)
                                                      aTAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag                                                                                        >  1:117296/1‑148 (MQ=255)
                                                        aaaCAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGAc                                                                                     >  2:131481/1‑149 (MQ=255)
                                                                       cGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAAt                                                                      <  1:131481/149‑1 (MQ=255)
                                                                           tCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATc                                                                  >  4:340119/1‑149 (MQ=255)
                                                                             aaGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCg                                                                 <  3:340119/148‑1 (MQ=255)
                                                                              aGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAg                                                               <  7:200907/149‑1 (MQ=255)
                                                                              aGGTTAGTGAGTATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAg                                                               <  7:206418/149‑1 (MQ=255)
                                                                               ggTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGTCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAgg                                                              <  8:34390/149‑1 (MQ=255)
                                                                                                 ccAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTc                                            >  1:189408/1‑149 (MQ=255)
                                                                                                 ccAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTc                                            >  4:170761/1‑149 (MQ=255)
                                                                                                     aTCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAgg                                                              >  4:176283/1‑127 (MQ=255)
                                                                                                     aTCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAgg                                                              <  3:176283/127‑1 (MQ=255)
                                                                                                         cccgccTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCttt                                    >  3:294507/1‑149 (MQ=255)
                                                                                                           cgccTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTtga                                  <  3:170761/149‑1 (MQ=255)
                                                                                                              cTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTtgatg                                >  2:152437/1‑148 (MQ=255)
                                                                                                                     aGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtctc                        <  5:213150/149‑1 (MQ=255)
                                                                                                                        aCCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTtga                                  >  6:280557/1‑136 (MQ=255)
                                                                                                                         ccGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATc                    <  1:257706/149‑1 (MQ=255)
                                                                                                                            gACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCtgt                 <  2:189408/149‑1 (MQ=255)
                                                                                                                               cAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCgc                                          <  2:288723/121‑1 (MQ=255)
                                                                                                                               cAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCgc                                          >  1:288723/1‑121 (MQ=255)
                                                                                                                                aGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTgaac             >  1:245346/1‑149 (MQ=255)
                                                                                                                                   aGTCGTGCGTAAATTAGCGCCCATTTCATTGGCGACAATGTTGGCCCGCGTAGTGTTTCCCAACCCCGGAGGCCCAAAAATCACCAAAGGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGAGGAAAATCTCCATCTGTgaacgaa          >  1:228766/1‑149 (MQ=255)
                                                                                                                                   aGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGATGGCAAGCGTAGTTTTACCCAACCACGGAGGATCAAAAATCAACAATAGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCGATCTGAgaacgag          >  3:24457/1‑148 (MQ=255)
                                                                                                                                           gTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGc  <  8:129994/149‑1 (MQ=255)
                                                                                                                                           gTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCACCATCTGTGAACGAACCTGCGGc  <  5:220936/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGC  >  NC_000913/1944968‑1945255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑