Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,619 A→G 9.7% intergenic (‑85/+27) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6190AG 9.7% 264.4 / 16.4 96intergenic (‑85/+27)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (50/34);  new base G (5/4);  total (56/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

TCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT  >  NC_000913/2311473‑2311767
                                                                                                                                                  |                                                                                                                                                    
tCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAgg                                                                                                                                                    >  7:282261/1‑149 (MQ=255)
    aaccaacAAAGAAGAGCGCTGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCt                                                                                                                                                <  5:219794/149‑1 (MQ=255)
    aaccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCt                                                                                                                                                >  4:56365/1‑149 (MQ=255)
     accaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCtt                                                                                                                                               >  2:146347/1‑149 (MQ=255)
       caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGCTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTg                                                                                                                                             >  8:238354/1‑149 (MQ=255)
        aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGTTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCACTTTGt                                                                                                                                            >  6:128482/1‑149 (MQ=255)
        aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                            >  3:155764/1‑149 (MQ=255)
           aaaGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGGGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                                                                          >  7:365008/1‑148 (MQ=255)
             agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTGATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                                        >  5:295293/1‑148 (MQ=255)
              gaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGata                                                                                                                                      >  7:75114/1‑149 (MQ=255)
               aagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGatat                                                                                                                                     <  8:282261/149‑1 (MQ=255)
                agagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                                                                    <  3:56365/149‑1 (MQ=255)
                 gagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                                                                    >  3:323461/1‑148 (MQ=255)
                   gcgcGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATGTCCATACGGGAGTATCCGCATGCGACCGGGCGCCAGAGTACCCCAAAAACCTGCGGGTGTACGCTGAAACCAATGCAAAAAGGGCCCGCCGGCCCTGGGTGCGAGATCact                                                                                                                                 >  1:45706/1‑147 (MQ=255)
                                 agTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTg                                                                                                                   <  8:75114/149‑1 (MQ=255)
                                  gTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                                  <  4:184888/149‑1 (MQ=255)
                                     ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCt                                                                                                                                                <  6:368862/116‑1 (MQ=255)
                                      cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                                              >  8:222276/1‑149 (MQ=255)
                                         gcgGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCa                                                                                                           <  7:270219/149‑1 (MQ=255)
                                          cgGGTAAAGCTTATTTCCATACGTGATTAACCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCAGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAg                                                                                                          >  3:37459/1‑149 (MQ=255)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                     <  3:292175/133‑1 (MQ=255)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcattg                                                                                                                                      <  7:311907/116‑5 (MQ=39)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcattg                                                                                                                                      >  8:311907/1‑112 (MQ=39)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcattg                                                                                                                                      <  5:145394/116‑5 (MQ=39)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcattg                                                                                                                                      >  6:145394/1‑112 (MQ=39)
                                               aaaGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                     >  4:292175/1‑133 (MQ=255)
                                                 aGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCaga                                                                                                   <  4:155764/149‑1 (MQ=255)
                                                  gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCGAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                                                                       >  5:54144/1‑112 (MQ=255)
                                                  gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCGAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                                                                       <  6:54144/112‑1 (MQ=255)
                                                    ttATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGc                                                                                                 >  8:348645/1‑148 (MQ=255)
                                                           cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTa                                                                                               >  7:47362/1‑143 (MQ=255)
                                                           cATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTa                                                                                               <  8:47362/143‑1 (MQ=255)
                                                           cATACGTGATTAACCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCCGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGt                                                                                         <  4:37459/149‑1 (MQ=255)
                                                             tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                       >  2:301921/1‑149 (MQ=255)
                                                                gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                    <  3:329693/149‑1 (MQ=255)
                                                                  gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                  >  5:26580/1‑149 (MQ=255)
                                                                    ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                                 <  7:222276/148‑1 (MQ=255)
                                                                      aTCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGAGtt                                                                               >  5:326167/1‑148 (MQ=255)
                                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                                 <  8:10144/143‑1 (MQ=255)
                                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtg                                                                                 >  7:10144/1‑143 (MQ=255)
                                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                            <  7:348645/148‑1 (MQ=255)
                                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                           >  2:204358/1‑149 (MQ=255)
                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                          >  5:92498/1‑149 (MQ=255)
                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACACAGAGCTACGATGTTATAAGTGTTGATGc                                                                          >  1:67697/1‑149 (MQ=255)
                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTCTACGCTGAAAACAATGAAAAAAGGGACCGAAGGCCCTTAGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTCTTGATGc                                                                          >  4:169576/1‑149 (MQ=255)
                                                                            aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                               <  5:162381/142‑1 (MQ=255)
                                                                            aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                               >  6:162381/1‑142 (MQ=255)
                                                                              gCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                      >  5:15261/1‑149 (MQ=255)
                                                                                gAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGCGCTACGATGTTATCAGTGTTGATGCCAGCGt                                                                    >  6:109011/1‑149 (MQ=255)
                                                                                     gTCGCAAGTGTCCCCCAAAATCCGGAGGTTGTACGCTGAAAACAATGCAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCACTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGCTGTTCGCAGTGTTGATGCCCGCGTCCCGa                                                               <  2:45706/149‑1 (MQ=255)
                                                                                          aaGAGTACCCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                          >  3:135686/1‑149 (MQ=255)
                                                                                           agagTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGTGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAAc                                                         <  1:275583/149‑1 (MQ=255)
                                                                                              gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                           >  7:157666/1‑112 (MQ=255)
                                                                                              gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                           <  8:157666/112‑1 (MQ=255)
                                                                                              gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                      <  4:323461/149‑1 (MQ=255)
                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                  >  6:135747/1‑149 (MQ=255)
                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                  >  8:232732/1‑149 (MQ=255)
                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                  >  7:297792/1‑149 (MQ=255)
                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                                                                        >  8:182906/1‑59 (MQ=25)
                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTcat                                                                                                                                        <  7:182906/61‑3 (MQ=25)
                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                                >  4:240393/1‑149 (MQ=255)
                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCACTTTGTTCGATATCAATCGAGATTAGCACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGGGTTGATGCCCGCGTCACCAGTGAACTTGTTgtcg                                                >  6:213477/1‑149 (MQ=255)
                                                                                                     aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                               >  3:261352/1‑149 (MQ=255)
                                                                                                      aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                               <  8:259511/116‑1 (MQ=255)
                                                                                                      aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                               >  7:259511/1‑116 (MQ=255)
                                                                                                      aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAAGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgacgtc                                              >  7:284678/1‑149 (MQ=255)
                                                                                                         aaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcag                                           >  5:146239/1‑149 (MQ=255)
                                                                                                          aCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagc                                          <  4:261352/149‑1 (MQ=255)
                                                                                                                  tGTACGCTTAAAACAATTAAAAAAGGTCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTTCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGa                                   <  8:284678/148‑1 (MQ=255)
                                                                                                                    tACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtt                                 <  1:301921/148‑1 (MQ=255)
                                                                                                                     aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                              >  4:352672/1‑134 (MQ=255)
                                                                                                                     aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                              <  3:352672/134‑1 (MQ=255)
                                                                                                                      cGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGAGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTg                              <  6:326167/149‑1 (MQ=255)
                                                                                                                        cTGAAAACAATGAATAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCGAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                            <  5:213477/149‑1 (MQ=255)
                                                                                                                        cTGAAAACAATGAACACAGGCCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGACCTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                            <  2:67697/149‑1 (MQ=255)
                                                                                                                         tGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACAGGTTGTCGTCCAGCAGGTTGATTTTg                              >  7:261220/1‑146 (MQ=255)
                                                                                                                           aaaaCATTGAAAACAGGGCCCGCTGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAg                           <  6:15261/147‑1 (MQ=255)
                                                                                                                           aaaaCAATGAAAAACGTGCCCGCTGTCCCTTTGTTCGATATCTATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                            <  8:261220/146‑1 (MQ=255)
                                                                                                                            aaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                         >  2:36826/1‑148 (MQ=255)
                                                                                                                                aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                            <  5:220961/93‑1 (MQ=255)
                                                                                                                                aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGAt                                                                            >  6:220961/1‑93 (MQ=255)
                                                                                                                                aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                     >  1:380759/1‑148 (MQ=255)
                                                                                                                                  tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                     >  7:386967/1‑50 (MQ=255)
                                                                                                                                  tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                     <  8:386967/50‑1 (MQ=255)
                                                                                                                                    aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                 <  5:135747/148‑1 (MQ=255)
                                                                                                                                    aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                >  2:92157/1‑149 (MQ=255)
                                                                                                                                     aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg               >  1:37887/1‑149 (MQ=255)
                                                                                                                                      aaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTg               >  4:317100/1‑148 (MQ=255)
                                                                                                                                       aaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa             >  8:35924/1‑149 (MQ=255)
                                                                                                                                       aaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa             >  4:145370/1‑149 (MQ=255)
                                                                                                                                        aaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa             >  4:258382/1‑148 (MQ=255)
                                                                                                                                          gggCCAGCAGGCCCGTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt          <  6:295293/149‑1 (MQ=255)
                                                                                                                                            gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt        <  3:240393/149‑1 (MQ=255)
                                                                                                                                              ccgccgGCCCTTTGTTCGATAGCAAGCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGCTGCCAGCGTCACGCGTGAACTGGTTGTCGTCCAGCAGGTGGATTTTGTAGTCAACGTAGGTGGACATGttt      <  4:135686/149‑1 (MQ=255)
                                                                                                                                              ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                               >  4:206978/1‑92 (MQ=255)
                                                                                                                                              ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                               <  3:206978/92‑1 (MQ=255)
                                                                                                                                              ccGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt      >  3:92373/1‑149 (MQ=255)
                                                                                                                                                  aGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCAAGCGTCACGAGTGAACTGGTTGTCGTCCAGAAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt  >  3:142617/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGT  >  NC_000913/2311473‑2311767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: