Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,200 G→A 5.8% intergenic (+43/+28) smrB → / ← yfcO putative DNA endonuclease/DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2000GA 5.8% 219.1 / 2.1 69intergenic (+43/+28)smrB/yfcOputative DNA endonuclease/DUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/38);  new base A (2/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

GCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGC  >  NC_000913/2449055‑2449348
                                                                                                                                                 |                                                                                                                                                    
gCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGATGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCTCCTGAGTTGCCCTGAGGAGTTGAGCTCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAAt                                                                                                                                                   >  1:48234/1‑149 (MQ=255)
gCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGGGATGCGGCGTGGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAAt                                                                                                                                                   <  2:260263/149‑1 (MQ=255)
   tgtgATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGcc                                                                                                                                                >  5:223791/1‑149 (MQ=255)
    gtgATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGccc                                                                                                                                               <  2:290534/149‑1 (MQ=255)
          gCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCATTCGCCcctgcc                                                                                                                                          <  4:217378/148‑1 (MQ=255)
           ccTTTCATCGGGCACCAAAAGAGTATGGCGGTGATGCGGCATTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCcctgcctg                                                                                                                                        <  8:323666/149‑1 (MQ=255)
             tttCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGgt                                                                                                                                      >  4:111293/1‑149 (MQ=255)
                 atcaGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTAt                                                                                                                                  <  8:309805/149‑1 (MQ=255)
                  tcaGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTa                                                                                                                                   >  5:36649/1‑147 (MQ=255)
                  tcaGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTa                                                                                                                                   <  6:36649/147‑1 (MQ=255)
                     ggCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTaa                                                                                                                              <  2:34049/149‑1 (MQ=255)
                        aCCAAAAGAGTATGGCGGTGATGCGGCGTTGTCGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAggg                                                                                                                           <  5:184087/149‑1 (MQ=255)
                          cAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGc                                                                                                                          >  1:130749/1‑148 (MQ=255)
                           aaaaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGATGAGTGGCTGCCGGCGGAGTTGCCCTGAGGAGTTGAGCGCGGTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTa                                                                                                                        <  1:254744/149‑1 (MQ=255)
                           aaaaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGTTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTa                                                                                                                        >  3:49400/1‑149 (MQ=255)
                           aaaaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCcctgcctg                                                                                                                                        <  5:108603/133‑1 (MQ=255)
                           aaaaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCcctgcctg                                                                                                                                        >  6:108603/1‑133 (MQ=255)
                            aaaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCTCGTTGCGCCAGGTACGTGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTAc                                                                                                                       <  2:88200/149‑1 (MQ=255)
                             aaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTAc                                                                                                                       >  3:218919/1‑148 (MQ=255)
                              agagTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACaa                                                                                                                     <  1:332297/149‑1 (MQ=255)
                               gagTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGGGTATCTAAGGGCTACAAc                                                                                                                    <  3:259485/149‑1 (MQ=255)
                                  tATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCa                                                                                                                 <  4:218919/149‑1 (MQ=255)
                                    tGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAtt                                                                                                               <  2:237315/149‑1 (MQ=255)
                                           gATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATg                                                                                                        >  8:48557/1‑149 (MQ=255)
                                                ggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGACGGAGTGGCCCTGAGGAGTTGAGAGAGGTGCGCCAGGTACGGTTAATGGCAGGGGCGCATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTg                                                                                                   >  2:372498/1‑149 (MQ=255)
                                                    tggttgGTGGTGATTGAAGGCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAAc                                                                                               <  8:268368/147‑1 (MQ=255)
                                                             ttgattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTa                                                                                      <  6:223791/149‑1 (MQ=255)
                                                             gtgcgtgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGCGGAGGTGCGCGCGTTGCGCGAGGAACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTa                                                                                      <  6:40597/144‑1 (MQ=255)
                                                               gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                         >  3:233083/1‑144 (MQ=255)
                                                               gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                         <  4:233083/144‑1 (MQ=255)
                                                               gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAg                                                                                     >  5:173615/1‑148 (MQ=255)
                                                                attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGaa                                                                                   >  1:176646/1‑149 (MQ=255)
                                                                attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGaa                                                                                   <  2:130749/149‑1 (MQ=255)
                                                                attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGa                                                                                    <  5:57962/148‑1 (MQ=255)
                                                                 ttgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGaa                                                                                   <  5:124460/148‑1 (MQ=255)
                                                                    aaGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGACCCGGCAAGTAGAATCGa                                                                               >  4:48805/1‑149 (MQ=255)
                                                                    aaGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGa                                                                               >  7:132729/1‑149 (MQ=255)
                                                                    aaGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGa                                                                               <  8:132729/149‑1 (MQ=255)
                                                                     aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                              >  7:85655/1‑149 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCt                                                                                                                                <  5:301983/95‑1 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCt                                                                                                                                >  6:301983/1‑95 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                         <  3:184756/134‑1 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                         >  4:184756/1‑134 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGc                                                                          >  4:168333/1‑149 (MQ=255)
                                                                         gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGc                                                                          >  8:54535/1‑149 (MQ=255)
                                                                               tGGTTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                    <  4:49400/149‑1 (MQ=255)
                                                                                   tGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGTTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGa                                                                               <  1:273861/134‑1 (MQ=255)
                                                                                   tGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGa                                                                               >  2:273861/1‑134 (MQ=255)
                                                                                     ccgccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATActgctg                                                              <  3:111293/149‑1 (MQ=255)
                                                                                       gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTgg                                                             >  5:111047/1‑148 (MQ=255)
                                                                                        ccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTa                                                           >  8:229523/1‑149 (MQ=255)
                                                                                          ggAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCaa                                                                                         <  6:349121/117‑1 (MQ=255)
                                                                                             gTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAac                                                      >  8:17682/1‑149 (MQ=255)
                                                                                                 cccTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgc                                                  <  7:229523/149‑1 (MQ=255)
                                                                                                    tGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgcac                                                >  8:48762/1‑148 (MQ=255)
                                                                                                        gagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                           <  3:48805/149‑1 (MQ=255)
                                                                                                              aGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgt                                     <  8:85655/149‑1 (MQ=255)
                                                                                                                ggcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGGATCTAAGGGCTACAACTCATTCCTTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAGACGCACGACCATATCgtgt                                   <  2:48234/148‑1 (MQ=255)
                                                                                                                  cgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTaa                                 <  7:48762/149‑1 (MQ=255)
                                                                                                                   gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGc                                                                       <  8:204733/110‑1 (MQ=255)
                                                                                                                   gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGc                                                                       >  7:204733/1‑110 (MQ=255)
                                                                                                                       cgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGt                             <  7:17682/148‑1 (MQ=255)
                                                                                                                       cgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGt                             <  3:168333/148‑1 (MQ=255)
                                                                                                                       cgcCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAAACGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGtt                            <  7:48557/149‑1 (MQ=255)
                                                                                                                           aGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGtg                        >  7:316272/1‑148 (MQ=255)
                                                                                                                                      caggCAGGGGCATTCGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATAc                                                                   <  6:376729/93‑1 (MQ=255)
                                                                                                                                      caggCAGGGGCATTCGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATAc                                                                   >  5:376729/3‑95 (MQ=255)
                                                                                                                                        ggCAGGGGCATTCGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGgtgt           >  3:171440/1‑149 (MQ=255)
                                                                                                                                               gCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTg    <  7:54535/149‑1 (MQ=255)
                                                                                                                                                 gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTGCGTTATAGGGTGTCGATTTGGc  <  8:316272/149‑1 (MQ=255)
                                                                                                                                                 gAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGc  <  6:173615/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGC  >  NC_000913/2449055‑2449348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: