Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,004,410 T→A 5.5% L459F (TTA→TTT ygeV ← putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,004,4100TA 5.5% 239.4 / 3.1 73L459F (TTA→TTTygeVputative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

TTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCTGATTAGTT  >  NC_000913/3004263‑3004551
                                                                                                                                                   |                                                                                                                                             
ttCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGt                                                                                                                                               >  5:102605/1‑148 (MQ=255)
     cTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAg                                                                                                                                         >  4:231676/1‑149 (MQ=255)
     cTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAg                                                                                                                                         >  8:143888/1‑149 (MQ=255)
      tGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGAAAAAAg                                                                                                                                         >  8:85249/1‑148 (MQ=255)
         aGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAAt                                                                                                                                     <  4:91980/149‑1 (MQ=255)
         aGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAAt                                                                                                                                     <  8:64691/149‑1 (MQ=255)
                      aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         >  7:235595/1‑148 (MQ=255)
                      aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         <  8:226292/148‑1 (MQ=255)
                      aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         <  8:235595/148‑1 (MQ=255)
                        cAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGAAAAAAGTAATGCACCAATAGTTCaa                                                                                                                      >  3:3982/1‑149 (MQ=255)
                         aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCaa                                                                                                                      >  2:50684/1‑148 (MQ=255)
                                ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTc                                                                                                               <  4:71590/148‑1 (MQ=255)
                                ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTc                                                                                                               <  8:75482/148‑1 (MQ=255)
                                ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGAAAAAAGTAATGCACCAATAGTTCAATATCTTc                                                                                                               <  7:85249/148‑1 (MQ=255)
                                 cATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCt                                                                                                             <  4:54319/149‑1 (MQ=255)
                                  aTCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                                                      >  2:58011/1‑123 (MQ=255)
                                       aTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGtt                                                                                                       >  3:310024/1‑149 (MQ=255)
                                        ttGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGtt                                                                                                       >  1:107484/1‑148 (MQ=255)
                                          gCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         >  3:157326/1‑128 (MQ=255)
                                          gCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         >  3:152198/1‑128 (MQ=255)
                                          gCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         <  4:152198/128‑1 (MQ=255)
                                          gCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                                                         <  4:157326/128‑1 (MQ=255)
                                           cTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCAc                                                                                                    <  1:20561/148‑1 (MQ=255)
                                           cTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACg                                                                                                   >  8:219442/1‑149 (MQ=255)
                                            ttAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATACACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACg                                                                                                   >  8:301841/1‑148 (MQ=255)
                                            ttAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACg                                                                                                   >  7:323810/1‑148 (MQ=255)
                                            ttAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTACGACGACATCCGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACg                                                                                                   >  8:274839/1‑148 (MQ=255)
                                              aaCTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGc                                                                                                  <  1:50684/147‑1 (MQ=255)
                                                     gcAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTg                                                                                           >  5:362641/1‑147 (MQ=255)
                                                     gcAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTg                                                                                           <  6:362641/147‑1 (MQ=255)
                                                     gcAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGAACCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCgg                                                                                             >  5:261732/1‑145 (MQ=255)
                                                     gcAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGAACCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCgg                                                                                             <  6:261732/145‑1 (MQ=255)
                                                       aGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTgg                                                                                          >  1:142959/1‑146 (MQ=255)
                                                        ggTTTCCGGGCCAACGATGCTTACGCAATATTTCTACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCaga                                                                                      <  5:1003/149‑1 (MQ=255)
                                                        ggTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCaga                                                                                      <  1:322819/149‑1 (MQ=255)
                                                        ggTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCaga                                                                                      <  6:102605/149‑1 (MQ=255)
                                                            tCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGtt                                                                                   <  7:143888/148‑1 (MQ=255)
                                                             ccGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTa                                                                                  <  8:323810/148‑1 (MQ=255)
                                                               gggCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCg                                                                               >  4:133222/1‑149 (MQ=255)
                                                                ggCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCgg                                                                              <  7:219442/149‑1 (MQ=255)
                                                                   cAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGAAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGAt                                                                           <  4:3982/149‑1 (MQ=255)
                                                                     aCGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATaa                                                                         <  2:48103/149‑1 (MQ=255)
                                                                             tACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACAAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGAAGAGTTATCGGGATAACATTAAGt                                                                 >  3:47916/1‑149 (MQ=255)
                                                                               cGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGAc                                                                                                          <  4:242303/106‑1 (MQ=255)
                                                                               cGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGAc                                                                                                          >  3:242303/1‑106 (MQ=255)
                                                                                gCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCgg                                                              >  7:174696/1‑149 (MQ=255)
                                                                                gCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCgg                                                              >  8:106347/1‑149 (MQ=255)
                                                                                gCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCgg                                                              >  8:100978/1‑149 (MQ=255)
                                                                                  aaTATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                                                      >  5:68187/1‑75 (MQ=255)
                                                                                  aaTATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                                                      <  6:68187/75‑1 (MQ=255)
                                                                                      tttCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaa                                                         <  3:231676/148‑1 (MQ=255)
                                                                                        tCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaaga                                                       <  2:107484/148‑1 (MQ=255)
                                                                                             gacATCTGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATTCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCtt                                                 <  7:274839/149‑1 (MQ=255)
                                                                                              acATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTc                                                <  3:133222/149‑1 (MQ=255)
                                                                                                aTCGGGAGCAATGCCAGGATAAGCCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATTGTTCAATATCTTGCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACAGTAAGTCGGGAGAAGAGATCTgcgc                                              >  2:139801/1‑149 (MQ=255)
                                                                                                aTCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGt                                                                 >  5:344523/1‑130 (MQ=255)
                                                                                                aTCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGt                                                                 <  6:344523/130‑1 (MQ=255)
                                                                                                        cAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                                                      >  7:304740/1‑53 (MQ=255)
                                                                                                        cAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                                                      <  8:304740/53‑1 (MQ=255)
                                                                                                          aTGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAAttt                                       <  3:81401/146‑1 (MQ=255)
                                                                                                          aTGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAAttt                                       >  4:81401/1‑146 (MQ=255)
                                                                                                          aTGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTAcc                                    >  7:125098/1‑149 (MQ=255)
                                                                                                              cattaTAATCCGTTCTCATATGAAGGGTTTGCAGATGTAAAAAGTTATGAACCAATATATCAATATCTTCCTGACGTTCACGAAGCGGTGTCAGAGTTATCGTGTTAATATTAAGTCTGTAGAAGAGATCTTCGCTTAATTTACCTTCg                                <  2:261314/145‑1 (MQ=255)
                                                                                                              cAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACAGTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCg                                >  6:147419/1‑149 (MQ=255)
                                                                                                                 gATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATATTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTAACCTTCGGc                              >  3:4768/1‑148 (MQ=255)
                                                                                                                      aCCGATCCCAGACGCCGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACAt                                                                      >  7:94936/1‑103 (MQ=255)
                                                                                                                      aCCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACAt                                                                      <  8:94936/103‑1 (MQ=255)
                                                                                                                       ccGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAAc                       >  7:253164/1‑149 (MQ=255)
                                                                                                                          aTCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTgg                    >  8:30051/1‑149 (MQ=255)
                                                                                                                          aTCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTgg                    >  1:28016/1‑149 (MQ=255)
                                                                                                                                gacgacGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCaaa              <  2:142959/149‑1 (MQ=255)
                                                                                                                                  cgacgGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAAtt            <  7:30051/149‑1 (MQ=255)
                                                                                                                                    acgGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCt          <  8:125098/149‑1 (MQ=255)
                                                                                                                                      gggTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCTGa        <  4:310024/149‑1 (MQ=255)
                                                                                                                                             cctatctAAAAAGTAATGCACAAATTGTTCAATATCTTCCTGTCGTTCACGCAGCGGTGTCTGAGTTATCGTGATTACATTTAGTCTGTATTAGTGATCTTCGCTGAATTTACCTTCTTCAATGTTCTGGGCCAAATTCTGATTAGtt  <  8:234532/142‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCTGATTAGTT  >  NC_000913/3004263‑3004551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: