Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,076,719 G→A 6.3% V246V (GTC→GTT)  yggP ← putative Zn‑binding dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,076,7190GA 6.3% 199.0 / 2.5 63V246V (GTC→GTT) yggPputative Zn‑binding dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base A (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

GAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTAT  >  NC_000913/3076572‑3076859
                                                                                                                                                   |                                                                                                                                            
gAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGa                                                                                                                                             >  2:13459/1‑149 (MQ=255)
                ggCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGACATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCCAACCCGGCATCTCCTGTCAGCACCCGCAGCATCTCGACAGGAACGCTCATccc                                                                                                                             >  6:33861/1‑149 (MQ=255)
                    cGGCATAGAAGTGCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGAGAGCAGGCACCGCCGCATAAACAAAAATGTCTTCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTg                                                                                                                         <  5:33861/149‑1 (MQ=255)
                    cGGCAAAGAAGCTCAGACAGCCATCTTCCGCCAGAAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTg                                                                                                                         <  5:311498/149‑1 (MQ=255)
                      gCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGt                                                                                                                       <  3:218349/149‑1 (MQ=255)
                        aaaGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGa                                                                                                                                        >  4:214661/1‑130 (MQ=255)
                        aaaGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGa                                                                                                                                        <  3:214661/130‑1 (MQ=255)
                          agaagTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTc                                                                                                                   <  1:13459/149‑1 (MQ=255)
                              gTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACAt                                                                                                               <  1:213016/149‑1 (MQ=255)
                               ttCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGAACAGGATCGCTCATCCCTTTGGTATTCACATa                                                                                                              >  1:203028/1‑149 (MQ=255)
                               ttCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGAACAGGATCGCTCATCCCTTTGGTATTCACATa                                                                                                              >  1:197122/1‑149 (MQ=255)
                                    aCAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTca                                                                                                                  <  6:93950/140‑1 (MQ=255)
                                    aCAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTca                                                                                                                  >  5:93950/1‑140 (MQ=255)
                                    aCAGCCATCTTCCGCAAGTAATTCCTCAGCCATCTCAACGACAGCCGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTTTTCACATACACCa                                                                                                         <  2:250091/149‑1 (MQ=255)
                                      aGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGc                                                                                                       >  6:370990/1‑149 (MQ=255)
                                               ccGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAAt                                                                                                  <  8:330014/145‑1 (MQ=255)
                                               ccGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAAt                                                                                                  <  3:106397/145‑1 (MQ=255)
                                                cGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCtt                                                                                             <  5:370990/149‑1 (MQ=255)
                                                 gCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCtt                                                                                             >  6:170542/1‑148 (MQ=255)
                                                   cAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTAc                                                                                          <  3:203256/149‑1 (MQ=255)
                                                         ttCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGgccg                                                                                    >  8:362464/1‑149 (MQ=255)
                                                          tcatcaGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGgccgc                                                                                   >  8:258703/1‑149 (MQ=255)
                                                               aGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCa                                                                                 <  4:65892/146‑1 (MQ=255)
                                                               aGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAAGGCCTTTACTGGCCGCCa                                                                                 >  3:65892/1‑146 (MQ=255)
                                                                gCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTc                                                                             >  8:104067/1‑149 (MQ=255)
                                                                   aTCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACCCCAGCTCACTGCCTTTACTGGCCGCCAGTTCCAc                                                                          >  1:144492/1‑149 (MQ=255)
                                                                        aaCGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCgg                                                                       >  7:201580/1‑147 (MQ=255)
                                                                        aaCGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCgg                                                                       >  7:43770/1‑147 (MQ=255)
                                                                        aaCGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGgca                                                                     >  4:305252/1‑149 (MQ=255)
                                                                           gACAGCAGGCACCGCCGCATAAACAAAAATGTCATCTAACCCGGCATCTCCTGTCAGCTCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGgcagca                                                                  <  4:283170/149‑1 (MQ=255)
                                                                                 agGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCt                                                            >  4:162462/1‑149 (MQ=255)
                                                                                  ggCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTg                                                           <  3:162462/149‑1 (MQ=255)
                                                                                   gCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGgc                                                                                      <  7:21273/121‑1 (MQ=255)
                                                                                   gCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGgc                                                                                      >  8:21273/1‑121 (MQ=255)
                                                                                   gCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTg                                                           >  6:224999/1‑148 (MQ=255)
                                                                                     aCCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGAACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTAc                                                        <  2:203028/149‑1 (MQ=255)
                                                                                     aCCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGAACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTAc                                                        <  2:197122/149‑1 (MQ=255)
                                                                                       cgccgcATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGgcag                                                                    <  2:269473/135‑1 (MQ=255)
                                                                                       cgccgcATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGgcag                                                                    >  1:269473/1‑135 (MQ=255)
                                                                                        gccgcATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTg                                                     >  7:264712/1‑149 (MQ=255)
                                                                                                aaCAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTg                                                                                                                         >  2:112437/1‑73 (MQ=255)
                                                                                                aaCAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTg                                                                                                                         <  1:112437/73‑1 (MQ=255)
                                                                                                      aaTGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCa                                                                                                         <  2:283245/83‑1 (MQ=255)
                                                                                                      aaTGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCa                                                                                                         >  1:283245/1‑83 (MQ=255)
                                                                                                          tCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTgtcgt                                    <  8:43770/148‑1 (MQ=255)
                                                                                                            aTCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGtcgtcg                                  <  5:224999/148‑1 (MQ=255)
                                                                                                            aTCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGa                                 >  7:51317/1‑149 (MQ=255)
                                                                                                             tCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGAc                                             <  2:368787/136‑1 (MQ=255)
                                                                                                             tCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGAc                                             >  1:368787/1‑136 (MQ=255)
                                                                                                                   ccGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATAAACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGac                          >  2:266297/1‑149 (MQ=255)
                                                                                                                     ggCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGt                                                          >  8:131282/1‑115 (MQ=255)
                                                                                                                        aTCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccacca                     <  7:104067/149‑1 (MQ=255)
                                                                                                                        aTCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccacca                     <  8:201580/149‑1 (MQ=255)
                                                                                                                           tcCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccaccacca                  >  7:98128/1‑149 (MQ=255)
                                                                                                                               gTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCcg              >  6:321711/1‑149 (MQ=255)
                                                                                                                                tCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGttt                                         >  7:9827/1‑121 (MQ=255)
                                                                                                                                tCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGttt                                         <  8:9827/121‑1 (MQ=255)
                                                                                                                                tCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccac                       <  1:302161/139‑1 (MQ=255)
                                                                                                                                tCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccac                       >  2:302161/1‑139 (MQ=255)
                                                                                                                                       ccGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATAAACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGtt      <  1:266297/149‑1 (MQ=255)
                                                                                                                                         gcagcaTCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGt    <  7:258703/149‑1 (MQ=255)
                                                                                                                                           agcaTCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTAt  <  5:321711/149‑1 (MQ=255)
                                                                                                                                           agcaTCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTAt  <  3:305252/149‑1 (MQ=255)
                                                                                                                                                   gACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccacc                      >  7:308206/1‑121 (MQ=255)
                                                                                                                                                   gACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGaccacc                      <  8:308206/121‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGGATCGCTCATCCCTTTGGTATTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTAT  >  NC_000913/3076572‑3076859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑