Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 276,134 G→T 9.9% V270V (GTG→GTT)  mmuP → CP4‑6 prophage; putative S‑methylmethionine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913276,1340GT 9.9% 207.2 / 11.8 71V270V (GTG→GTT) mmuPCP4‑6 prophage; putative S‑methylmethionine transporter
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base T (3/4);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

ATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTCGAAT  >  NC_000913/275997‑276281
                                                                                                                                         |                                                                                                                                                   
aTTGCCGCCGGTGAAACGGAAAACCCGCGCTAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATGTTCTTTATCGGCCACCTGTTTGTGCTGGCAGCGCAGCTCCCGATGCAGCAGTTGGGCGGGGAGAAAAGccc                                                                                                                                          >  3:230299/1‑149 (MQ=255)
aTTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGccc                                                                                                                                          >  8:287462/1‑149 (MQ=255)
    ccggcgGGGAAACGGAAAACCCGCGCAAAGTTATCCCGGAATCGATTAGTACTAGCATCGCGAGACTGATTATTTTCTTTATCGGCACCTTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGttt                                                                                                                                      <  4:230299/149‑1 (MQ=255)
     cgccgGTGAAACGGAAAACCAGCGCAAACTTATCCCGGGAGCGACTCGTACTACCATCGCGCGACTGAATATTATCTTTATCGGCACCGTGTTTGTGCTGGCCGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTtg                                                                                                                                     <  1:229635/149‑1 (MQ=255)
               aCGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTAtt                                                                                                                           >  7:277541/1‑149 (MQ=255)
               aCGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTAGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTAtt                                                                                                                           >  4:254475/1‑149 (MQ=255)
                cGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTATCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTAttt                                                                                                                          <  4:280072/149‑1 (MQ=255)
                cGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTAttt                                                                                                                          <  8:198185/149‑1 (MQ=255)
                 ggAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTAttt                                                                                                                          >  1:52988/1‑148 (MQ=255)
                     aaCCCGCCCAAAGTTATCCCGCTAGCTATTCGTACTACCATCGCGCGACTGATAATTTTCTTTATCGGCAACGTGTTTCTGCTGGCAGAGCTGAAACCGATCCACCAGGAGGGCGTGAAGAAAATCCCGTTTCTGca                                                                                                                                 >  8:274778/1‑136 (MQ=255)
                      aCCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTtgt                                                                                                                                    >  8:13553/1‑133 (MQ=255)
                      aCCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTtgt                                                                                                                                    <  7:13553/133‑1 (MQ=255)
                      aCCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTg                                                                                                                                >  5:56235/1‑137 (MQ=255)
                      aCCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTg                                                                                                                                <  6:56235/137‑1 (MQ=255)
                              aaaGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTATTTATCGGCACAGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGATGGTATTTTAGAAAGTAGGGAt                                                                                                            >  5:79981/1‑149 (MQ=255)
                                  ttATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCg                                                                                                        <  2:52988/149‑1 (MQ=255)
                                           tAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTga                                                                                                                        >  1:320363/1‑124 (MQ=255)
                                           tAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTga                                                                                                                        <  2:320363/124‑1 (MQ=255)
                                               gATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGaaaa                                                                                                                                              <  3:147271/98‑1 (MQ=255)
                                               gATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGaaaa                                                                                                                                              >  4:147271/1‑98 (MQ=255)
                                                      actacCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTaa                                                                                    >  7:159494/1‑149 (MQ=255)
                                                        tacCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGaaa                                                                                                                    <  6:153526/115‑1 (MQ=255)
                                                        tacCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGaaa                                                                                                                    >  5:153526/1‑115 (MQ=255)
                                                        tacCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACt                                                                                  >  3:320150/1‑149 (MQ=255)
                                                          actacgcgcgACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTc                                                                                <  7:321684/145‑1 (MQ=255)
                                                           cATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGCTGCAGCAGGTGGGCGTGGAGAACCGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCg                                                                               <  5:95958/149‑1 (MQ=255)
                                                            aTCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGt                                                                              >  8:235911/1‑149 (MQ=255)
                                                            aTCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTCATATTTTTAACTTCGt                                                                              >  5:288040/1‑149 (MQ=255)
                                                            aTCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGAGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATAttttt                                                                                      >  7:256801/1‑141 (MQ=255)
                                                            aTCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGGTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATAttttt                                                                                      <  8:256801/141‑1 (MQ=255)
                                                                      tGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATcc                                                                                                          <  4:321990/111‑1 (MQ=255)
                                                                      tGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATcc                                                                                                          >  3:321990/1‑111 (MQ=255)
                                                                       gATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACgg                                                                   >  7:312144/1‑149 (MQ=255)
                                                                         ttattTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGc                                                                  >  7:149752/1‑148 (MQ=255)
                                                                           attTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAt                                                               >  1:382166/1‑149 (MQ=255)
                                                                            ttttCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAtt                                                              >  7:251101/1‑149 (MQ=255)
                                                                             tttCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttc                                                             >  6:212237/1‑149 (MQ=255)
                                                                               tCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctt                                                           >  7:207906/1‑149 (MQ=255)
                                                                               tCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttct                                                            <  8:277541/148‑1 (MQ=255)
                                                                                 tttATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGa                                                                      <  7:27389/136‑1 (MQ=255)
                                                                                 tttATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGa                                                                      >  8:27389/1‑136 (MQ=255)
                                                                                   tATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttcttt                                                          <  8:312144/146‑1 (MQ=255)
                                                                                   tATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctttct                                                        <  4:320150/148‑1 (MQ=255)
                                                                                   tATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctttc                                                         <  3:254475/147‑1 (MQ=255)
                                                                                   tATCGGCACCGTGTTGGGGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGGTTGGGCTGGTATTTGAGAAAGTCGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctttc                                                         <  8:188685/147‑1 (MQ=255)
                                                                                      cGGCACCGGGTTTGGGCGGGCAGCGCGGATCCCGTTGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCa                                                     <  6:288040/148‑1 (MQ=255)
                                                                                       ggCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGc                                                   >  6:205968/1‑149 (MQ=255)
                                                                                        gCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGc                                                   >  3:138393/1‑148 (MQ=255)
                                                                                         cACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCg                                                  >  3:132669/1‑148 (MQ=255)
                                                                                          aCCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGaa                                                <  8:159494/149‑1 (MQ=255)
                                                                                                  tgtgCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCggg                                         <  5:205968/148‑1 (MQ=255)
                                                                                                    tgCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGtt                                       <  7:287462/148‑1 (MQ=255)
                                                                                                     gCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTta                                      >  3:72272/1‑148 (MQ=255)
                                                                                                       tGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGACAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTTCAGCGAACAGCGGGTAatat                                   >  3:62650/1‑149 (MQ=255)
                                                                                                         gCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGc                                 >  2:184425/1‑149 (MQ=255)
                                                                                                               cTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCgg                           <  4:138393/149‑1 (MQ=255)
                                                                                                                tGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCACTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGtt                                       <  7:209207/136‑1 (MQ=255)
                                                                                                                tGATCCCGATGCAGCAGGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCACTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGtt                                       >  8:209207/1‑136 (MQ=255)
                                                                                                                  aTCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctttct                                                        >  3:190981/1‑117 (MQ=255)
                                                                                                                  aTCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTAttctttct                                                        <  4:190981/117‑1 (MQ=255)
                                                                                                                   tCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGgcg                        >  3:219713/1‑148 (MQ=255)
                                                                                                                         ttcaggagGTGGGGGGGGAGAAAAGCCCGTTTGTGCTGGTATTTGTGAAAGTAGGGATCCCGTACGCCGCTTATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCtg                 <  8:207906/147‑1 (MQ=255)
                                                                                                                           cagcagGTGGGCGTGGAGAATAGCCCGTTTGTTCTGGTATTGGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCtgtg               <  4:62650/149‑1 (MQ=255)
                                                                                                                              cagGTGGGCGTTGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTc            <  8:251101/149‑1 (MQ=255)
                                                                                                                               agGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCg           <  4:219713/149‑1 (MQ=255)
                                                                                                                                 gTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGtt         <  8:149752/149‑1 (MQ=255)
                                                                                                                                    ggCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGt       <  7:235911/148‑1 (MQ=255)
                                                                                                                                    ggCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTc      <  4:132669/149‑1 (MQ=255)
                                                                                                                                       gTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTCGaa   <  1:184425/149‑1 (MQ=255)
                                                                                                                                        tGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTCGAAt  <  5:212237/149‑1 (MQ=255)
                                                                                                                                        tGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTCGAAt  >  4:103043/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
ATTGCCGCCGGTGAAACGGAAAACCCGCGCAAAGTTATCCCGGTAGCGATTCGTACTACCATCGCGCGACTGATTATTTTCTTTATCGGCACCGTGTTTGTGCTGGCAGCGCTGATCCCGATGCAGCAGGTGGGCGTGGAGAAAAGCCCGTTTGTGCTGGTATTTGAGAAAGTAGGGATCCCGTACGCCGCTGATATTTTTAACTTCGTGATCCTGACGGCTATTCTTTCTGCAGCGAACTCCGGGTTATATGCCTCCGGGCGCATGCTGTGGTCGTTGTCGAAT  >  NC_000913/275997‑276281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑