Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,222,778 | A→G | 5.3% | N49S (AAT→AGT) | ebgA → | evolved beta‑D‑galactosidase, alpha subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,222,778 | 0 | A | G | 5.3% | 239.2 / 3.2 | 76 | N49S (AAT→AGT) | ebgA | evolved beta‑D‑galactosidase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base A (36/36); new base G (2/2); total (38/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01 |
CCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTC > NC_000913/3222630‑3222923 | ccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa > 5:186917/1‑149 (MQ=255) ccTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGaa > 2:377/1‑149 (MQ=255) cTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAAt > 6:52989/1‑149 (MQ=255) aaTCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCa < 5:88484/149‑1 (MQ=255) gCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtt < 5:52989/148‑1 (MQ=255) gggAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACtttttt > 8:279523/1‑149 (MQ=255) ggAAAACATTCATCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCTCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGTAATTTCCACtttttt < 1:348929/148‑1 (MQ=255) ggAAAACATTCAGCGCACCCCCGTAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGCTGCGCAAGCGCGTTCCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTACGCGGGCAGTGGAATTTCCACttttt < 1:373506/147‑1 (MQ=255) aaaaCATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAc > 8:36228/1‑149 (MQ=255) aaaaCATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGAc < 1:377/149‑1 (MQ=255) cATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGATGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATc < 1:118195/149‑1 (MQ=255) ttCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAGTTTCCACTTTTTTGACCATCCg > 2:152996/1‑149 (MQ=255) aGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTg > 1:172826/1‑149 (MQ=255) gCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAGTTTCCACTTTTTTGACCATCCGCTGc < 1:152996/149‑1 (MQ=255) aCCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAg > 5:158461/1‑148 (MQ=255) ccACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTAc > 6:277020/1‑149 (MQ=255) gAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGa > 6:101999/1‑128 (MQ=255) gAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGa < 5:101999/128‑1 (MQ=255) gAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAg > 7:23659/1‑148 (MQ=255) aCTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCa < 2:172826/149‑1 (MQ=255) cgccgcGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACctc < 7:279523/148‑1 (MQ=255) gccgcGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACctct > 1:68902/1‑148 (MQ=255) gcgtgcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGt < 7:337125/149‑1 (MQ=255) gcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACctct > 2:263382/1‑141 (MQ=255) gcgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACctct < 1:263382/141‑1 (MQ=255) cgtACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATg < 2:11333/149‑1 (MQ=255) aCTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGCCCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCt > 8:349507/1‑149 (MQ=255) aCTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa > 5:30736/1‑139 (MQ=255) aCTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa < 6:30736/139‑1 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 8:165328/1‑149 (MQ=255) tttttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa > 8:323184/1‑149 (MQ=255) tttttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGa < 7:36228/147‑1 (MQ=255) tttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTg < 2:68902/148‑1 (MQ=255) ttCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggg > 3:266588/1‑149 (MQ=255) tCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggg < 7:165328/148‑1 (MQ=255) atGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCaa < 2:282608/104‑1 (MQ=255) atGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCaa > 1:282608/1‑104 (MQ=255) gATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCat < 1:308426/147‑1 (MQ=255) gATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCat > 2:308426/1‑147 (MQ=255) tGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCg > 2:241764/1‑149 (MQ=255) ttGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGt < 6:186917/148‑1 (MQ=255) ttGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTc > 1:80085/1‑149 (MQ=255) gcgTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAGTTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa > 4:228636/1‑108 (MQ=255) gcgTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAGTTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGa < 3:228636/108‑1 (MQ=255) gTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATg > 3:288536/1‑113 (MQ=255) gTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATg < 4:288536/113‑1 (MQ=255) cTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACTATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCaaa > 2:105819/1‑149 (MQ=255) cTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggg < 7:53170/119‑1 (MQ=255) cTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggg > 8:53170/1‑119 (MQ=255) tGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATg < 2:80085/148‑1 (MQ=255) cccGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTTACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGaa < 6:158461/149‑1 (MQ=255) cccGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATgg > 1:299908/1‑147 (MQ=255) cccGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGaa < 8:23659/149‑1 (MQ=255) cccGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGaa < 8:16058/149‑1 (MQ=255) ccGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAg > 4:149579/1‑149 (MQ=255) gcgAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGt > 4:13410/1‑149 (MQ=255) aaaCCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACTATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCAc < 1:105819/149‑1 (MQ=255) aCCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACgg < 7:323184/149‑1 (MQ=255) ccTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATgg > 4:39274/1‑89 (MQ=255) ccTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATgg < 3:39274/89‑1 (MQ=255) cTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCAGCCATGTGGCAAATGGAAGGTCACGGCAAACTGc > 3:25555/1‑148 (MQ=255) tttCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggggg < 8:273016/96‑1 (MQ=255) tttCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTggggg > 7:273016/1‑96 (MQ=255) ttCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAata > 2:89707/1‑148 (MQ=255) cTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAAtata < 1:241764/148‑1 (MQ=255) cccTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACcga > 8:74804/1‑149 (MQ=255) ccTTAAGCTGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACcgac > 2:194687/1‑149 (MQ=255) cTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGaa > 7:349107/1‑121 (MQ=255) cTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGaa < 8:349107/121‑1 (MQ=255) gCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCTTCCCCGCCATGTGGAAAATGGAAGGACACGGCAAACTGCGAAAAACCGACGATGGt > 3:53485/1‑149 (MQ=255) tCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTc < 3:13410/149‑1 (MQ=255) gTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCaaa < 2:39548/121‑1 (MQ=255) gTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCaaa > 1:39548/1‑121 (MQ=255) tGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAat < 2:231432/127‑1 (MQ=255) tGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAat > 1:231432/1‑127 (MQ=255) tGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACcg < 8:160196/133‑1 (MQ=255) tGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACcg > 7:160196/1‑133 (MQ=255) gAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAATTGCAATATACCGACGAAGGTTTTCCGTTc < 3:149579/148‑1 (MQ=255) aTTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCaa < 6:74162/122‑1 (MQ=255) aTTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCaa > 5:74162/1‑122 (MQ=255) | CCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTC > NC_000913/3222630‑3222923 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |