Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,795 A→G 5.5% intergenic (‑51/+32) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,7950AG 5.5% 218.4 / 2.7 74intergenic (‑51/+32)ygjV/uxaAImp‑YgjV family inner membrane protein/altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

GCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTC  >  NC_000913/3241648‑3241934
                                                                                                                                                   |                                                                                                                                           
gctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAc                                                                                                                                            >  4:275520/1‑149 (MQ=255)
  tttttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCATAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                                          >  2:114168/1‑149 (MQ=255)
  tttttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                                          >  3:261787/1‑149 (MQ=255)
        aaGCGTCGTTCGTCACGTTTGAAAATTGTTGTGATACCAATCAGAAATGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGttt                                                                                                                                     <  6:364712/148‑1 (MQ=255)
        aaGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGtttt                                                                                                                                    >  8:2297/1‑149 (MQ=255)
           cGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                                 <  4:233030/149‑1 (MQ=255)
               gTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaa                                                                                                                             <  3:176420/149‑1 (MQ=255)
               gTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaa                                                                                                                             <  4:261787/149‑1 (MQ=255)
                ttCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                            <  7:2297/149‑1 (MQ=255)
                ttCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                            <  3:275520/149‑1 (MQ=255)
                    tCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACt                                                                                                                        >  6:340226/1‑149 (MQ=255)
                             aaaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                               >  3:283594/1‑149 (MQ=255)
                             aaaaaTGTTGTGATACCAATAAGAAAGGCGATGCCACCCACGCACTGGGCCAGACAATACGCGGTAATGAGAAAACCGTCGCAGGAATGGAAAAGCAAACGGAGCTTCACATTATGAAACGAAGGTTGTTATTAAAAACTCATTTCGAc                                                                                                               >  1:103822/1‑149 (MQ=255)
                              aaacTGTTGTGATACCAATCAGAAAGGCGATGACACACACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAACCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACt                                                                                                              <  4:5642/149‑1 (MQ=255)
                               aaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                             >  1:84906/1‑149 (MQ=255)
                                aaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGACAATACGCGGTAATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                             >  3:67938/1‑148 (MQ=255)
                                      gtgAAACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGc                                                                                                       <  3:182485/148‑1 (MQ=255)
                                                  aGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                            <  5:325835/115‑1 (MQ=255)
                                                  aGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                            >  6:325835/1‑115 (MQ=255)
                                                      aGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGaa                                                                                      <  7:380279/149‑1 (MQ=255)
                                                          gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTg                                                                                        >  7:168456/1‑143 (MQ=255)
                                                          gATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATc                                                                                  <  2:84906/149‑1 (MQ=255)
                                                            tGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATTATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                <  1:114168/149‑1 (MQ=255)
                                                             gACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                >  4:201347/1‑148 (MQ=255)
                                                              acacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCa                                                                              <  8:168456/149‑1 (MQ=255)
                                                                acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                >  1:162310/1‑145 (MQ=255)
                                                                acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                <  2:162310/145‑1 (MQ=255)
                                                                acCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCATTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGt                                                                            >  7:145830/1‑149 (MQ=255)
                                                                  ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCTCATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTc                                                                          >  6:360128/1‑149 (MQ=255)
                                                                  ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTCTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTc                                                                          >  6:61839/1‑149 (MQ=255)
                                                                  ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTCCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                <  2:155185/143‑1 (MQ=255)
                                                                  ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTCCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGc                                                                                >  1:155185/1‑143 (MQ=255)
                                                                  ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCGTAGCAGGTATGGAAAAGCAAACGCCGCTTCACATTATGAACCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGt                                                                            >  2:288034/1‑147 (MQ=255)
                                                                        gcTGGGCCAGCCAATCCTCTGTCATGCGACATCATTAGCAGGGATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACTACGCTTTTGAATATCGACATTTATCGGaa                                                                    <  2:103822/148‑1 (MQ=255)
                                                                        ccTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGaa                                                                    <  3:201347/149‑1 (MQ=255)
                                                                                           ggTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                                 <  5:340226/149‑1 (MQ=255)
                                                                                               aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                                 <  3:9183/65‑1 (MQ=255)
                                                                                               aTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                                 >  4:9183/1‑65 (MQ=255)
                                                                                                  aTAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                                          >  5:90136/1‑149 (MQ=255)
                                                                                                   tAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                                 <  6:186569/61‑1 (MQ=255)
                                                                                                     aaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                                        <  6:90136/148‑1 (MQ=255)
                                                                                                      aTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                               <  2:17596/140‑1 (MQ=255)
                                                                                                      aTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                               >  1:17596/1‑140 (MQ=255)
                                                                                                       tacTTAGCCTGTCTGGAAAAGCAAACGGCGCTGCACATTAGGAAAAGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACCCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAc                                       <  1:213764/145‑1 (MQ=255)
                                                                                                       tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                                       >  2:261554/1‑99 (MQ=255)
                                                                                                       tCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGa                                                                                       <  1:261554/99‑1 (MQ=255)
                                                                                                       tCCTTAGCAGGTATGGAAAAGCAAACGGCGCGTCACAGTATGATACGCCGTTGTTTATGAACAACTCATTTAGACTTTATAGCGTTACGCCGCGTTTGAAGATCGCAAGATCGCGGAAGTCGTTACGCTCTTTACATGTTTGCTTACCg                                     >  1:68009/1‑149 (MQ=255)
                                                                                                       accTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACATTATAGCGTTACGCCGCTTTAGAAGATCGACAGTTCGCGGAAGTCTTTACGCTCGTTACAGGTTTGCttcc                                       >  2:213764/2‑145 (MQ=255)
                                                                                                            aGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTgg                                 >  6:370716/1‑148 (MQ=255)
                                                                                                            aGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                                  <  5:61839/147‑1 (MQ=255)
                                                                                                             gCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGc                                <  4:67938/148‑1 (MQ=255)
                                                                                                                 gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                                        >  2:83935/1‑120 (MQ=255)
                                                                                                                 gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                                        <  1:83935/120‑1 (MQ=255)
                                                                                                                 gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                                 >  5:258018/1‑127 (MQ=255)
                                                                                                                 gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAg                                                 <  6:258018/127‑1 (MQ=255)
                                                                                                                  tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                                        <  5:192018/135‑1 (MQ=255)
                                                                                                                  tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTa                                        >  6:192018/1‑135 (MQ=255)
                                                                                                                    tGGAAAAGCAAACGGCGCTTCTCATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa                        <  5:360128/149‑1 (MQ=255)
                                                                                                                      gAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAAc                       <  4:283594/148‑1 (MQ=255)
                                                                                                                      gAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAAc                       >  2:98615/1‑148 (MQ=255)
                                                                                                                         taaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                   >  8:174388/5‑149 (MQ=39)
                                                                                                                           gCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgt                 >  8:378610/1‑149 (MQ=255)
                                                                                                                             aaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgtgt               <  7:174388/149‑1 (MQ=255)
                                                                                                                              aaCGGCGTGTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTATGAATATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTAGGCAAACTCAAAGATGGTGTc              >  1:108560/1‑149 (MQ=255)
                                                                                                                                       tCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttc     >  6:309100/1‑149 (MQ=255)
                                                                                                                                         acaTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttctt   >  7:112035/1‑149 (MQ=255)
                                                                                                                                          caTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc  <  5:309100/149‑1 (MQ=255)
                                                                                                                                           aTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGtt                                              >  8:52586/1‑104 (MQ=255)
                                                                                                                                           aTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGtt                                              <  7:52586/104‑1 (MQ=255)
                                                                                                                                           aTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc  >  7:297985/1‑148 (MQ=255)
                                                                                                                                           aTTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCACCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAAttcttc  >  8:156089/1‑148 (MQ=255)
                                                                                                                                            ttATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCaa                        <  8:103385/125‑1 (MQ=255)
                                                                                                                                              aTGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                               >  2:108429/1‑100 (MQ=255)
                                                                                                                                              aTGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGt                                               <  1:108429/100‑1 (MQ=255)
                                                                                                                                                   aCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                   >  2:164896/1‑123 (MQ=255)
                                                                                                                                                   aCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATg                   <  1:164896/123‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATAAATTCTTC  >  NC_000913/3241648‑3241934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: