Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,383,300 T→G 5.4% intergenic (+33/+30) degS → / ← mdh serine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,383,3000TG 5.4% 224.6 / 4.0 74intergenic (+33/+30)degS/mdhserine endoprotease, periplasmic/malate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base G (2/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACG  >  NC_000913/3383157‑3383447
                                                                                                                                                |                                                                                                                                                   
gATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTcc                                                                                                                                                >  6:22100/1‑149 (MQ=255)
         gCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTtta                                                                                                                                       <  4:209868/149‑1 (MQ=255)
         gCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTtta                                                                                                                                       <  3:165341/149‑1 (MQ=255)
           ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTttat                                                                                                                                      >  2:367297/1‑148 (MQ=255)
           ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTttat                                                                                                                                      >  4:229280/1‑148 (MQ=255)
           ggAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCAACATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTatat                                                                                                                                      >  1:11701/1‑148 (MQ=255)
                     ccTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAAt                                                                                                                           >  5:120793/1‑149 (MQ=255)
                         gTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCaa                                                                                                                        <  8:261342/148‑1 (MQ=255)
                               tGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACtta                                                                                                                 <  2:380901/149‑1 (MQ=255)
                                  tCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                              <  4:79618/149‑1 (MQ=255)
                                   cccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTaa                                                                                                             >  7:364083/1‑149 (MQ=255)
                                    ccTGTAGTAGTGATGCGTGATGATAAGCATTTAACGCTGCAGGTCACGATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                                            <  3:206754/149‑1 (MQ=255)
                                    ccTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAAc                                                                                                            <  4:354060/149‑1 (MQ=255)
                                     cTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGtttttt                                                                                                                                        >  1:304470/1‑120 (MQ=38)
                                     cTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGtttttt                                                                                                                                        <  2:304470/120‑1 (MQ=38)
                                        tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                         >  2:42984/1‑116 (MQ=255)
                                        tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                         <  1:42984/116‑1 (MQ=255)
                                        tagtagTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGaa                                                                                                         <  8:136052/148‑1 (MQ=255)
                                          gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAActc                                                                                                      >  8:280873/1‑149 (MQ=255)
                                          gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGTTTTttgttttga                                                                                                                                 >  8:122311/1‑118 (MQ=37)
                                          gtagtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGCACAGACTCCGGTTTTttgttttga                                                                                                                                 <  7:122311/122‑5 (MQ=37)
                                             gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                         <  2:146487/111‑1 (MQ=255)
                                             gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGttttt                                                                                                                                         >  1:146487/1‑111 (MQ=255)
                                             gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                              <  2:305298/138‑1 (MQ=255)
                                             gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACttatta                                                                                                              >  1:305298/1‑138 (MQ=255)
                                             gtGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGCGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTc                                                                                                   <  1:299163/149‑1 (MQ=255)
                                                  gCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGcc                                                                                                >  5:52543/1‑147 (MQ=255)
                                                    gTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAgg                                                                                            >  1:288436/1‑149 (MQ=255)
                                                         gatAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGa                                                                                        >  5:165378/1‑148 (MQ=255)
                                                             aGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtct                                                                                   >  7:30589/1‑149 (MQ=255)
                                                              gCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctt                                                                                  >  3:21935/1‑149 (MQ=255)
                                                              gCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctt                                                                                  >  7:18044/1‑149 (MQ=255)
                                                                aGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttc                                                                                <  8:18044/149‑1 (MQ=255)
                                                                     aCGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCa                                                                            <  6:120793/148‑1 (MQ=255)
                                                                     aCGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTAATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAg                                                                           >  1:132918/1‑149 (MQ=255)
                                                                       gCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCg                                                                         >  8:99758/1‑149 (MQ=255)
                                                                         tGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTAATATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTa                                                                       >  3:173870/1‑149 (MQ=255)
                                                                            aGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtcttt                                                                                 <  4:31067/136‑1 (MQ=255)
                                                                            aGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtcttt                                                                                 >  3:31067/1‑136 (MQ=255)
                                                                              gTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAg                                                                  <  2:11701/149‑1 (MQ=255)
                                                                                 aCCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAAGTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTCTCCAGCa                                                                >  5:5409/1‑148 (MQ=255)
                                                                                   cATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttctt                                                                              <  7:333238/132‑1 (MQ=255)
                                                                                   cATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATAtctttctt                                                                              >  8:333238/1‑132 (MQ=255)
                                                                                    aTTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATAcc                                                            <  5:22100/149‑1 (MQ=255)
                                                                                     ttCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCt                                                           >  5:54783/1‑149 (MQ=255)
                                                                                        aGGAATATCCGGCAACCAATTAAGTCTTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGTTTTTTTATTATCCGATAATAAATTACTTATTAAAGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCATCATaaatta                                                         >  8:192865/1‑143 (MQ=255)
                                                                                        aGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCg                                                                                                                                <  8:267582/77‑1 (MQ=255)
                                                                                        aGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCg                                                                                                                                >  7:267582/1‑77 (MQ=255)
                                                                                          gAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAAACCGGAGGGTGTGCGCCGGTTTTTTTTTATCCGCTATTCAATTCGTTATTAACGAAGTCTTCGCCCAGGGCGATAGCTTTCTTCAGCGTATCCAGCATACCTTc                                                         >  1:247820/1‑147 (MQ=255)
                                                                                             tatCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgcgc                                                   >  3:102858/1‑149 (MQ=255)
                                                                                               tCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGt                                                 >  7:248561/1‑149 (MQ=255)
                                                                                                 cGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTc                                               <  3:229280/149‑1 (MQ=255)
                                                                                                  ggCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCt                                              <  8:30589/149‑1 (MQ=255)
                                                                                                     aaCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGtt                                           <  4:21935/149‑1 (MQ=255)
                                                                                                     aaCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTAATATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGtt                                           <  4:173870/149‑1 (MQ=255)
                                                                                                      aCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTc                                          <  6:52543/149‑1 (MQ=255)
                                                                                                      aCCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTc                                          <  7:280873/149‑1 (MQ=255)
                                                                                                       ccAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCa                                         <  1:367297/149‑1 (MQ=255)
                                                                                                           ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgc                                                     >  3:162586/1‑133 (MQ=255)
                                                                                                           ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAgc                                                     <  4:162586/133‑1 (MQ=255)
                                                                                                           ttAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATg                                     >  3:342674/1‑149 (MQ=255)
                                                                                                                     cgcTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAggg                            <  8:364083/148‑1 (MQ=255)
                                                                                                                        tCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTAcc                        >  8:384508/1‑149 (MQ=255)
                                                                                                                        tCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTAcc                        >  2:144345/1‑149 (MQ=255)
                                                                                                                            aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGat                                                                                       >  6:95501/1‑82 (MQ=255)
                                                                                                                            aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGat                                                                                       <  5:95501/82‑1 (MQ=255)
                                                                                                                            aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGa                      >  3:174037/1‑147 (MQ=255)
                                                                                                                            aacaaaaAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATa                    <  7:99758/149‑1 (MQ=255)
                                                                                                                                aaaaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt                 >  2:170401/1‑149 (MQ=255)
                                                                                                                                aaaaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt                 >  3:7262/1‑149 (MQ=255)
                                                                                                                                  aaaaCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAttt               <  6:54783/149‑1 (MQ=255)
                                                                                                                                    aaCCGGAGGCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTAc             <  2:247820/149‑1 (MQ=255)
                                                                                                                                     aCCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACg            >  3:167451/1‑149 (MQ=255)
                                                                                                                                      caggAGTCTGTGCGCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCCGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGc           <  6:5409/147‑1 (MQ=255)
                                                                                                                                               gtgCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACg  >  7:218990/1‑149 (MQ=255)
                                                                                                                                               gtgCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCCTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt                 <  4:210275/134‑1 (MQ=255)
                                                                                                                                               gtgCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCATCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGAt                 >  3:210275/1‑134 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GATCAGGTGGCGGAAATTCGCCCTGGTTCGGTGATCCCTGTAGTAGTGATGCGTGATGATAAGCAGTTAACGCTGCAGGTCACCATTCAGGAATATCCGGCAACCAATTAAGTCGTGCGCTCAAAACAAAAAACCGGAGTCTGTGCTCCGGTTTTTTATTATCCGCTAATCAATTACTTATTAACGAACTCTTCGCCCAGGGCGATATCTTTCTTCAGCGTATCCAGCATACCTTCCAGCGCGTTCTGTTCAAATGCGCTCAGGGTACCGATAGATTTACGCTCTTCCACG  >  NC_000913/3383157‑3383447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: