Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,473,626 T→C 5.0% A152A (GCA→GCG rpsG ← 30S ribosomal subunit protein S7

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,473,6260TC 5.0% 250.9 / 2.6 79A152A (GCA→GCGrpsG30S ribosomal subunit protein S7
Reads supporting (aligned to +/- strand):  ref base T (35/40);  new base C (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATC  >  NC_000913/3473478‑3473773
                                                                                                                                                    |                                                                                                                                                   
tGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAgtgt                                                                                                                                                     <  1:114315/149‑1 (MQ=255)
  ttACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTGCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGtgtg                                                                                                                                                    <  2:339262/148‑1 (MQ=255)
          gCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAgtgt                                                                                                                                                     <  3:310335/139‑1 (MQ=255)
          gCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAgtgt                                                                                                                                                     >  4:310335/1‑139 (MQ=255)
          gCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCt                                                                                                                                           <  1:148255/149‑1 (MQ=255)
               cGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCttgttg                                                                                                                                      >  6:96052/1‑149 (MQ=255)
               cGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCttgtt                                                                                                                                       <  1:158262/148‑1 (MQ=255)
                  tGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGcc                                                                                                                                            <  3:126307/140‑1 (MQ=255)
                  tGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGcc                                                                                                                                            >  4:126307/1‑140 (MQ=255)
                           tACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATa                                                                                                                          >  5:48804/1‑149 (MQ=255)
                              gAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGc                                                                                                                              <  6:87557/142‑1 (MQ=255)
                              gAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGc                                                                                                                              >  5:87557/1‑142 (MQ=255)
                               aGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                                                                                      <  7:272546/149‑1 (MQ=255)
                               aGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                                                                                      <  7:168707/149‑1 (MQ=255)
                                 ccATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGCGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                                                                                     >  8:236391/1‑148 (MQ=255)
                                 ccATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGCGCGAACGCCTTGTTGGCTTCGGCCATACGGTg                                                                                                                     >  8:230604/1‑148 (MQ=255)
                                  cATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                                                                                        <  2:181889/144‑1 (MQ=255)
                                  cATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACg                                                                                                                        >  1:181889/1‑144 (MQ=255)
                                   aTTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                                                                                  <  2:297624/149‑1 (MQ=255)
                                   aTTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                                                                                  <  5:96052/149‑1 (MQ=255)
                                    tttGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACg                                                                                                                 >  2:78737/1‑149 (MQ=255)
                                       gTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTc                                                                                                               <  1:78737/148‑1 (MQ=255)
                                        tttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCtt                                                                                                             >  4:965/1‑149 (MQ=255)
                                        tttCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCt                                                                                                              <  1:201024/148‑1 (MQ=255)
                                            cTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTc                                                                                                                                 <  3:271480/125‑1 (MQ=255)
                                            cTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTc                                                                                                                                 >  4:271480/1‑125 (MQ=255)
                                            cTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTAACg                                                                                                         >  7:294961/1‑149 (MQ=255)
                                                   aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                                                                                      <  5:82210/129‑1 (MQ=255)
                                                   aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGt                                                                                                                      >  6:82210/1‑129 (MQ=255)
                                                   aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                                                                                   <  7:161247/148‑1 (MQ=255)
                                                   aTCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTACGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCtt                                                                                                   >  7:180185/1‑148 (MQ=255)
                                                      ttttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCttgtt                                                                                                                                       >  3:296328/1‑109 (MQ=255)
                                                      ttttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCttgtt                                                                                                                                       <  4:296328/109‑1 (MQ=255)
                                                      ttttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACt                                                                                               >  1:308921/1‑149 (MQ=255)
                                                        ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTaa                                                                                                 <  7:65908/145‑1 (MQ=255)
                                                        ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTg                                                                                              <  2:308921/148‑1 (MQ=255)
                                                        ttAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTg                                                                                              <  6:48804/148‑1 (MQ=255)
                                                           ggCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAg                                                                                           >  5:362007/1‑148 (MQ=255)
                                                              gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                                                                                       <  3:120603/133‑1 (MQ=255)
                                                              gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGtt                                                                                                       >  4:120603/1‑133 (MQ=255)
                                                              gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAcc                                                                                       >  7:146180/1‑149 (MQ=255)
                                                              gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAcc                                                                                       >  2:55760/1‑149 (MQ=255)
                                                              gTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTAcc                                                                                       >  4:4521/1‑149 (MQ=255)
                                                                        aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt                                                                             <  3:163878/149‑1 (MQ=255)
                                                                        aGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCt                                                                             <  5:287011/149‑1 (MQ=255)
                                                                          tAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGc                                                                                                                                                   <  1:369693/77‑1 (MQ=255)
                                                                          tAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGc                                                                                                                                                   >  2:369693/1‑77 (MQ=255)
                                                                             cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagc                                                                        <  3:4521/149‑1 (MQ=255)
                                                                             cccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcag                                                                         >  8:9635/1‑148 (MQ=255)
                                                                              ccAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTgcagca                                                                       >  8:130987/1‑149 (MQ=255)
                                                                                aaaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCAt                                                                      >  3:177038/1‑148 (MQ=255)
                                                                                aaaGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATc                                                                     <  1:295883/149‑1 (MQ=255)
                                                                                     gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTTCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa                                                                <  7:204023/149‑1 (MQ=255)
                                                                                     gggCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGaaa                                                                <  3:965/149‑1 (MQ=255)
                                                                                           ctTACTGGAAGCGCCCGCCTGGTGACTTAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCAATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttc                                                            <  7:249310/147‑1 (MQ=255)
                                                                                           ctTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttc                                                            <  8:146180/147‑1 (MQ=255)
                                                                                            ttACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                                                                                  >  5:37046/1‑92 (MQ=255)
                                                                                            ttACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAAc                                                                                                                  <  6:37046/92‑1 (MQ=255)
                                                                                              aCTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAgttcgtt                                                         <  7:9635/147‑1 (MQ=255)
                                                                                                  gAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCa                                                    <  1:55760/148‑1 (MQ=255)
                                                                                                       gcccgccTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGCGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCa                                              <  7:230604/149‑1 (MQ=255)
                                                                                                       gcccgccTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGCGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCa                                              <  7:236391/149‑1 (MQ=255)
                                                                                                                gTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATgg                                     <  8:294961/149‑1 (MQ=255)
                                                                                                                gTGACTAAAACTCCGAAGCGAAAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTACGCCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTCTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCACAGCCAtc                                      >  8:49706/1‑147 (MQ=255)
                                                                                                                 tGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGa                                    <  7:130987/149‑1 (MQ=255)
                                                                                                                 tGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGa                                    <  6:362007/149‑1 (MQ=255)
                                                                                                                     tAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTa                                >  5:175238/1‑149 (MQ=255)
                                                                                                                     tAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTa                                >  3:39452/1‑149 (MQ=255)
                                                                                                                     tAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTGTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTa                                >  8:329337/1‑149 (MQ=255)
                                                                                                                       aaaCTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTAt                               >  6:5694/1‑148 (MQ=255)
                                                                                                                        aaCTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCa                             >  7:29694/1‑149 (MQ=255)
                                                                                                                        aaCTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCa                             >  4:331063/1‑149 (MQ=255)
                                                                                                                              gAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGt                       <  6:175238/149‑1 (MQ=255)
                                                                                                                                    aTAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGa                 <  4:177038/149‑1 (MQ=255)
                                                                                                                                       aCCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGagc               <  5:5694/148‑1 (MQ=255)
                                                                                                                                        ccAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGagcag             >  4:11772/1‑149 (MQ=255)
                                                                                                                                        ccAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGa                 >  2:224964/1‑145 (MQ=255)
                                                                                                                                        ccAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGa                 <  1:224964/145‑1 (MQ=255)
                                                                                                                                         cAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGagca              >  4:198144/1‑147 (MQ=255)
                                                                                                                                         cAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGagca              <  3:198144/147‑1 (MQ=255)
                                                                                                                                         cAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAACTTCGTTCGCCAGGCGCAGAGCCATGGATTTATAACCGCGTTTACGagcagc            >  7:182199/1‑149 (MQ=255)
                                                                                                                                              gTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCaa       >  5:173075/1‑149 (MQ=255)
                                                                                                                                               tAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAAc      <  7:49706/149‑1 (MQ=255)
                                                                                                                                                   gtgCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATc  >  4:331409/1‑149 (MQ=255)
                                                                                                                                                    tgCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATc  >  2:320574/1‑148 (MQ=255)
                                                                                                                                                    tgCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATc  >  4:143903/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGTTACGGTAGCGTGCGATGGGTGTTGTACGAGCCATTTGTTTCCTCGTTTATCTTTTAGGCGTTCAATTTAAGTAGCCCAAAGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATC  >  NC_000913/3473478‑3473773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: