Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,485,143 G→A 6.6% A218A (GCG→GCA prkB → putative phosphoribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,485,1430GA 6.6% 288.4 / 6.2 91A218A (GCG→GCAprkBputative phosphoribulokinase
Reads supporting (aligned to +/- strand):  ref base G (42/43);  new base A (3/3);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTACCGGGCGGCAAAATGG  >  NC_000913/3484996‑3485291
                                                                                                                                                   |                                                                                                                                                    
cAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCgg                                                                                                                                                     >  8:154997/1‑149 (MQ=255)
cAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCgg                                                                                                                                                     >  2:271360/1‑149 (MQ=255)
cAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCgg                                                                                                                                                     <  7:154997/149‑1 (MQ=255)
 agcgagcgCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCACGCACCCATCTTAACATCCAGCGCGTTCCCACCGTCGACACTTCAAAACCGATCGCgg                                                                                                                                                     >  2:354413/1‑148 (MQ=255)
     agcgCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCaaaa                                                                                                                                                <  4:270111/149‑1 (MQ=255)
     agcgCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCaaaa                                                                                                                                                <  5:192107/149‑1 (MQ=255)
      gcgcGGGCACTCACGAGAAGCAGTGATGGACACAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCAAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAg                                                                                                                                               >  1:368963/1‑149 (MQ=255)
          gggCACTCACGAGAAGCTGTGATGGAATCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCCGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTAt                                                                                                                                           <  6:232325/149‑1 (MQ=255)
          gggCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGCCACTTCAAACCCGTTCGCGGCAAAAGGTAt                                                                                                                                           >  6:57970/1‑149 (MQ=255)
          gggCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTAt                                                                                                                                           <  1:131921/149‑1 (MQ=255)
           ggCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATc                                                                                                                                          <  4:231358/149‑1 (MQ=255)
                tCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCaaaa                                                                                                                                                >  8:59972/1‑138 (MQ=255)
                tCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCaaaa                                                                                                                                                <  7:59972/138‑1 (MQ=255)
                  aCGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGc                                                                                                                                   <  1:354413/149‑1 (MQ=255)
                               aTGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCtt                                                                                                                      <  3:11895/149‑1 (MQ=255)
                                tGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCttt                                                                                                                     >  2:185332/1‑149 (MQ=255)
                                  gACTCAGTAGTGCGTTCAATGTAAGTCTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTtg                                                                                                                    <  5:307235/148‑1 (MQ=255)
                                   aCTCAGTAGTGCGTTCAATGGAAGACTATATGAAGTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTtgtg                                                                                                                  <  7:345654/149‑1 (MQ=255)
                                       agttgtGAGTTCATTGGAAGCATATTTCAACTACCTCACACCGCAGTTTTCCCGCAGCCATCTTACCTTCCGCCCCGTTCCCACCGTCGCCGCATCCCACCCGTTCGCGGCATCAGGTATGCCGTCGGTCGCTCAAAGCTTTGTGGTGa                                                                                                              >  2:46942/1‑149 (MQ=255)
                                       agtagtGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGACAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGa                                                                                                              >  6:323767/1‑149 (MQ=255)
                                           gtGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGa                                                                                                                               >  6:200082/1‑128 (MQ=255)
                                           gtGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGa                                                                                                                               <  5:200082/128‑1 (MQ=255)
                                           gtGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCAt                                                                                                         >  5:231129/1‑149 (MQ=255)
                                           gtGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCa                                                                                                          >  5:194053/1‑149 (MQ=255)
                                              cGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCAttt                                                                                                       <  4:75539/149‑1 (MQ=255)
                                                  cAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCg                                                                                                    <  6:194053/148‑1 (MQ=255)
                                                   aaTGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGt                                                                                                   >  3:23379/1‑148 (MQ=255)
                                                       gAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGACAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCt                                                                                              <  5:323767/149‑1 (MQ=255)
                                                          gACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTgg                                                                                            >  4:28309/1‑148 (MQ=255)
                                                            cTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAg                                                                                         <  1:271360/149‑1 (MQ=255)
                                                             tataTCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAgg                                                                                        >  1:305605/1‑149 (MQ=255)
                                                             tataTCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAgg                                                                                        >  1:285555/1‑149 (MQ=255)
                                                               tatCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGa                                                                                      <  2:285555/149‑1 (MQ=255)
                                                                atCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGa                                                                                      <  2:305605/148‑1 (MQ=255)
                                                                 tCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          <  5:88847/143‑1 (MQ=255)
                                                                 tCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGAt                                                                                     <  1:185332/148‑1 (MQ=255)
                                                                 tCAACTACATCACACAGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          >  6:88847/1‑143 (MQ=255)
                                                                   aaCTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGaaa                                                                                                                          <  7:336524/109‑1 (MQ=255)
                                                                   aaCTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGaaa                                                                                                                          >  8:336524/1‑109 (MQ=255)
                                                                   aaCTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGa                                                                                  >  3:24655/1‑149 (MQ=255)
                                                                     cTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGAttt                                                                               <  6:231129/149‑1 (MQ=255)
                                                                        cATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTcc                                                                             >  3:331542/1‑149 (MQ=255)
                                                                        cATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTc                                                                              <  3:28309/148‑1 (MQ=255)
                                                                          tCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          >  3:124113/1‑134 (MQ=255)
                                                                          tCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          <  4:124113/134‑1 (MQ=255)
                                                                          tCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGa                                                                                  <  4:148480/142‑1 (MQ=255)
                                                                          tCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGa                                                                                  >  3:148480/1‑142 (MQ=255)
                                                                              acCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTgg                                                                        >  1:286195/1‑148 (MQ=255)
                                                                              acCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTgg                                                                        <  2:286195/148‑1 (MQ=255)
                                                                              acCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgc                                                                       >  4:198933/1‑149 (MQ=255)
                                                                               ccGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTg                                                                                             >  3:341186/1‑126 (MQ=255)
                                                                               ccGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTg                                                                                             <  4:341186/126‑1 (MQ=255)
                                                                                cGCAGTTTTCCTGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTc                                                                                                                                     >  1:341279/1‑85 (MQ=255)
                                                                                cGCAGTTTTCCTGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTc                                                                                                                                     <  2:341279/85‑1 (MQ=255)
                                                                                cGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgctg                                                                     >  6:28622/1‑149 (MQ=255)
                                                                                 gCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgctgc                                                                    >  3:288492/1‑149 (MQ=255)
                                                                                 gCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgctgc                                                                    <  3:198933/149‑1 (MQ=255)
                                                                                   aGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGAt                                                                                                                              >  8:50031/1‑89 (MQ=255)
                                                                                   aGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGAt                                                                                                                              <  7:50031/89‑1 (MQ=255)
                                                                                    gTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTgg                                                                 <  4:24655/149‑1 (MQ=255)
                                                                                          ccGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCt                                                                          >  8:352/1‑134 (MQ=255)
                                                                                          ccGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCt                                                                          <  7:352/134‑1 (MQ=255)
                                                                                          ccGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGt                                                           <  4:288492/149‑1 (MQ=255)
                                                                                            gCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTg                                                         <  5:28622/149‑1 (MQ=255)
                                                                                              aCCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGc                                                        >  7:211610/1‑148 (MQ=255)
                                                                                                ccATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          >  3:123286/1‑112 (MQ=255)
                                                                                                ccATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGaa                                                                                          <  4:123286/112‑1 (MQ=255)
                                                                                                  aTCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGc                                                   >  8:208226/1‑149 (MQ=255)
                                                                                                       aaCTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCt                                                  <  7:208226/145‑1 (MQ=255)
                                                                                                          ttacAGCGCGTTCCCACCGTCGACACTTCAAAACCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGAGTTTGCAAGGAtcattcat                                           >  6:495/4‑149 (MQ=255)
                                                                                                          ttCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCtcattcat                                           >  8:219540/1‑149 (MQ=255)
                                                                                                             cAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGAt                                                                                     <  4:118470/104‑1 (MQ=255)
                                                                                                             cAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGAt                                                                                     >  3:118470/1‑104 (MQ=255)
                                                                                                              aGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCttt                                                                                                                     <  1:199882/71‑1 (MQ=255)
                                                                                                              aGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCttt                                                                                                                     >  2:199882/1‑71 (MQ=255)
                                                                                                               gcgcGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGAt                                                             <  8:292904/126‑1 (MQ=255)
                                                                                                               gcgcGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGAt                                                             >  7:292904/1‑126 (MQ=255)
                                                                                                                   gTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCa                                                       <  8:356444/128‑1 (MQ=255)
                                                                                                                   gTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCa                                                       >  7:356444/1‑128 (MQ=255)
                                                                                                                       ccACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCaa                               >  1:76851/1‑148 (MQ=255)
                                                                                                                        cACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgc                                                                       <  4:227673/107‑1 (MQ=255)
                                                                                                                        cACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgc                                                                       >  3:227673/1‑107 (MQ=255)
                                                                                                                           cGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGt                          <  8:211610/149‑1 (MQ=255)
                                                                                                                               gACTCTTCAAACCCGTTCGCGGCAAAATGTATCCCTTAGGTCGATGAAAGCGTTGTGGTGATCCATTTTCGTAATCTGGAATGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGTCTCATTCATTTCCCACATCAATACGTTAGt                      <  5:495/149‑1 (MQ=255)
                                                                                                                               gACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGt                          <  2:273153/145‑1 (MQ=255)
                                                                                                                               gACACTTCAAACCCGTTCGCAGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGt                          >  1:273153/1‑145 (MQ=255)
                                                                                                                                acacTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTg                     >  4:82851/1‑149 (MQ=255)
                                                                                                                                    ttCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGctgctg                                                                  >  7:228528/1‑100 (MQ=255)
                                                                                                                                    ttCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGctgctg                                                                  <  8:228528/100‑1 (MQ=255)
                                                                                                                                    ttCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTAc                 >  5:3380/1‑149 (MQ=255)
                                                                                                                                    ttCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTACGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTAc                 >  5:78218/1‑149 (MQ=255)
                                                                                                                                       aaaCCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGATTTGTGCTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAATGCTCATTCATTTCACACATCAATACGTTAGTGGTACCgg              >  1:298151/1‑149 (MQ=255)
                                                                                                                                        aaCCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgctgc                                                                    <  7:325547/94‑1 (MQ=255)
                                                                                                                                        aaCCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGgctgc                                                                    >  8:325547/1‑94 (MQ=255)
                                                                                                                                          cccGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTATCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCTACATCAATACGTTAGTGGTACCGggcg           <  6:316731/149‑1 (MQ=255)
                                                                                                                                                 gcgGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTACCGGGCGGCAAAAt    >  1:216422/1‑149 (MQ=255)
                                                                                                                                                   ggCAAAAGGTATCCCGTGGCTCCATGAAATCTTTGTGGTGCTCCATTTTCGTAATCTGGAAGTGATCGATTTCCCCTTGGTGCTGGCTATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTTACGGGCGGCAAAATgc  <  2:368963/149‑2 (MQ=255)
                                                                                                                                                   ggCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTACCGGGCGGCAAAATgg  <  3:82851/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCAGTAGTGCGTTCAATGGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTTCAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTCGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATCGATTTCCCCTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTACCGGGCGGCAAAATGG  >  NC_000913/3484996‑3485291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: