Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,806,430 C→A 9.0% R213L (CGC→CTC)  waaG ← UDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,806,4300CA 9.0% 309.1 / 7.4 101R213L (CGC→CTC) waaGUDP‑glucose:(heptosyl)lipopolysaccharide alpha‑1,3‑glucosyltransferase; lipopolysaccharide core biosynthesis protein; lipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base C (57/34);  new base A (2/8);  total (59/42)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ATTGCGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAAT  >  NC_000913/3806282‑3806578
                                                                                                                                                    |                                                                                                                                                    
atTGCGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAACGCGTATTGTGCCGTAATGCTTCCGGTACCGCTGCCAAAGCTTCAATTGAGc                                                                                                                                                      >  5:301871/1‑149 (MQ=255)
    cGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGGTTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATc                                                                                                                                                  <  8:238849/149‑1 (MQ=255)
    cGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGAt                                                                                                                                                   <  3:193555/148‑1 (MQ=255)
    cGACCGGAGAAGAAATGCACATGGCTCCGCACGCCGAGATTTTCTGCCAGCGCCTCAAATTTTCGCGGCTTATCCTGACCAACAACAACTAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGAt                                                                                                                                                   <  2:84121/148‑1 (MQ=255)
                    gCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATACTGACAAACAAAAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAAAGATGCCAAAGCTTCAATTGAGAGATCTACACCTTTACGGcc                                                                                                                                   >  5:372835/1‑148 (MQ=255)
                      acaTTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCaa                                                                                                                                 >  2:327822/1‑148 (MQ=255)
                        aatGCTCCGCACCCCGAGTGTTTCTGCCAGCTCCTCCAATTTTCGCGGCTTATCCTGACCCACAACAATTAAAAGCGTTTTGTGACGTATTGATTTTTTTTTTTTTTTGATAGGGTGAATTGAGAGATCTACACCTTTACGGCCAAAAt                                                                                                                              <  6:28372/147‑1 (MQ=255)
                        aTTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAAt                                                                                                                              >  8:195267/1‑149 (MQ=255)
                        aTTCCTCCGCAAGCCGAGATTTTCTGCCAGCGCTACAAATTTTCGCGGCTTATCCAGACAAACAACAAATAAAAGCGTATTATGACCTAATGAATCAGGTAACGATGCCAAAGATACAATTGAGCGATCTACACCTTTACGGCCaaaa                                                                                                                               >  6:85386/1‑148 (MQ=255)
                         ttGCTCCGCACGCCGAGTTATTCTGCCATCGCTTCAAATTTACCCGGCTTATCCTGACCAACAACAAATAAAACCGTAATGTGACGTAATGATTCCTGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATc                                                                                                                             >  8:100726/1‑149 (MQ=255)
                            cTCCGCACGCCGAGTTTTTGTGCCAGAGATTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTTACTTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGa                                                                                                                          <  1:253615/149‑1 (MQ=255)
                             tCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACATTTACGTCCAAAATCTGa                                                                                                                          >  3:221675/1‑148 (MQ=255)
                                    gCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCt                                                                                                                  >  8:8402/1‑149 (MQ=255)
                                    gCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCt                                                                                                                  >  8:315432/1‑149 (MQ=255)
                                    gCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCt                                                                                                                  >  8:153195/1‑149 (MQ=255)
                                       gAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATTAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGACCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCa                                                                                                               <  5:85386/149‑1 (MQ=255)
                                         gTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGTGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAg                                                                                                              >  8:15530/1‑148 (MQ=255)
                                               cTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTGCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGtaataa                                                                                                        <  3:111240/148‑1 (MQ=255)
                                                  ccAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGTGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGttt                                                                                                    <  7:15530/149‑1 (MQ=255)
                                                   cAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGtttt                                                                                                   >  2:195071/1‑149 (MQ=255)
                                                    aGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGtttgg                                                                                                  >  3:278738/1‑147 (MQ=255)
                                                     gcgcTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttgt                                                                                                 <  7:153195/149‑1 (MQ=255)
                                                     gagcTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttgt                                                                                                 <  1:327822/147‑1 (MQ=255)
                                                         ttCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTTACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGAGATCTCCACCTTTACGGCCAAAATCTGAAACAACCTGAAGTAATAAGTTttgtttc                                                                                              >  4:24265/1‑146 (MQ=255)
                                                          tCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGtaat                                                                                                          >  6:250856/1‑135 (MQ=255)
                                                          tCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGtaat                                                                                                          <  5:250856/135‑1 (MQ=255)
                                                          tCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTCCACTAATAAGTGTTGTTtctc                                                                                            >  6:19826/1‑149 (MQ=255)
                                                             aaTTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCttt                                                                                         <  7:98940/149‑1 (MQ=255)
                                                             aaTTTTCGCGGCATATCCTGACCAACAACAAATAAAAGCGTATTGAGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCttt                                                                                         >  6:220532/1‑149 (MQ=255)
                                                               ttttCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttg                                                                                                  <  7:375969/138‑1 (MQ=255)
                                                               ttttCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttg                                                                                                  >  8:375969/1‑138 (MQ=255)
                                                                        cTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAAc                                                                                                                    >  3:310976/1‑111 (MQ=255)
                                                                            tCCTGACCAACAACAAATAACAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                          <  7:369616/149‑1 (MQ=255)
                                                                            tCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                          >  4:141844/1‑149 (MQ=255)
                                                                            tCCTGACAAACAACAAATAAAATCGTATTGTGACGTAATGATTTTTTTTTTTTTTTGATAGGTTGAATTGAGAGATATACACCTTTACGGCCAAAATCTGATCAAACGTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCt                                                                           <  4:216042/148‑1 (MQ=255)
                                                                             ccTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTgg                                                                         >  7:333288/1‑149 (MQ=255)
                                                                              cTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTgg                                                                         <  7:315432/148‑1 (MQ=255)
                                                                              cTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGc                                                                        >  7:80512/1‑149 (MQ=255)
                                                                               tGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                          >  2:299554/1‑146 (MQ=255)
                                                                               tGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                          <  1:299554/146‑1 (MQ=255)
                                                                               tGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTATTTTATGCCATTTTTCTGGc                                                                        >  5:305973/1‑148 (MQ=255)
                                                                               tGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTATGTTGCTCTTTTATGCCATTTTTCTGGc                                                                        >  1:139876/1‑148 (MQ=255)
                                                                               tGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTACGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGc                                                                        >  7:180137/1‑148 (MQ=255)
                                                                               cctatcgcaacaaATAACAGCGTATTGTGACGTAATGATTTGTTTTTTTTTTTGATAGGTTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTg                                                                          <  2:261365/139‑1 (MQ=255)
                                                                                    aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAATGCTTCAATTGAGCGATCTac                                                                                                                                               <  6:29832/72‑1 (MQ=255)
                                                                                    aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAGGCTTCAATTGAGCGATCTac                                                                                                                                               >  5:29832/1‑72 (MQ=255)
                                                                                    aacaacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATaaa                                                                  <  7:8402/149‑1 (MQ=255)
                                                                                       aacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAAttt                                                               >  6:222042/1‑149 (MQ=255)
                                                                                       aacaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGAGATaggaag                                                               >  1:261365/1‑144 (MQ=255)
                                                                                        acaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAAttt                                                               >  8:187273/1‑148 (MQ=255)
                                                                                        acaaATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTc                                                              <  3:224150/149‑1 (MQ=255)
                                                                                            aTAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGACAAAATCTGATCCAACCTGCAGTAATATGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACgg                                                          >  6:319028/1‑149 (MQ=255)
                                                                                             tAAAAGCGTATTGTGACGTAATGATTCTTTTATAGATTTGAAAGCTTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTg                                                                                                                 <  2:118427/93‑1 (MQ=255)
                                                                                             tAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTg                                                                                                                 >  1:118427/1‑93 (MQ=255)
                                                                                              aaaaGCGTATTGTGACGTAATGCTTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTGACGGCt                                                        >  3:238621/1‑149 (MQ=255)
                                                                                              aaaaGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCt                                                        >  7:111600/1‑149 (MQ=255)
                                                                                               aaaGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                        <  3:66616/116‑1 (MQ=255)
                                                                                               aaaGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCtttt                                                                                        >  4:66616/1‑116 (MQ=255)
                                                                                                 aGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGtt                                                     >  6:245293/1‑149 (MQ=255)
                                                                                                       ttGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTGCTGGCGATAAATTTCACGGCTGTTTGGGa                                                <  1:195071/148‑1 (MQ=255)
                                                                                                          tgACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTg                                            <  5:245293/149‑1 (MQ=255)
                                                                                                           gccGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCAAAAATCTGATCCAACGTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGc                                           >  7:268448/3‑149 (MQ=255)
                                                                                                              gTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCa                                        >  3:97358/1‑149 (MQ=255)
                                                                                                              gTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCa                                        >  2:319073/1‑149 (MQ=255)
                                                                                                               tAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                                  <  5:97695/138‑1 (MQ=255)
                                                                                                               tAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                                  >  6:97695/1‑138 (MQ=255)
                                                                                                                aaTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                      >  6:154662/1‑149 (MQ=255)
                                                                                                                aaTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                      >  2:374632/1‑149 (MQ=255)
                                                                                                                aaTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                      >  8:3710/1‑149 (MQ=255)
                                                                                                                 aTGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCTAAATCTTATCCAACCTGCAGTAATAAGTTTTGTTGCGCTTTTCTGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACt                                      <  7:100726/148‑1 (MQ=255)
                                                                                                                    aTTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTAt                                  >  5:1198/1‑149 (MQ=255)
                                                                                                                        tggTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttgt                                                                                                 >  8:372888/2‑82 (MQ=255)
                                                                                                                        tggTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTttgt                                                                                                 <  7:372888/81‑1 (MQ=255)
                                                                                                                          gTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTg                            >  7:340769/1‑149 (MQ=255)
                                                                                                                          gTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTg                            >  3:196525/1‑149 (MQ=255)
                                                                                                                              ctattccAAAGGTTGAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTcc                         <  8:268448/143‑1 (MQ=255)
                                                                                                                               gATGCCAAAGCTTCAATTGAGCGATCTACTCCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCg                        >  1:182695/1‑148 (MQ=255)
                                                                                                                               gATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCGCTTGTATGCCAGTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCg                        >  5:327148/1‑148 (MQ=255)
                                                                                                                                    cAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATaaa                  >  7:161170/1‑149 (MQ=255)
                                                                                                                                    cAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATaaa                  >  6:249970/1‑149 (MQ=255)
                                                                                                                                    cAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCAGTTTGGGATTTGCTCACTGTAATTTCTGTCCGGATaaa                  >  8:326324/1‑149 (MQ=255)
                                                                                                                                     aaaGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATaaa                  >  6:256975/1‑148 (MQ=255)
                                                                                                                                      taGCTTGAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATa                <  4:278738/148‑1 (MQ=255)
                                                                                                                                       aGCTTCAATTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATAc               <  8:333288/149‑1 (MQ=255)
                                                                                                                                        gCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATAcc              <  5:154662/149‑1 (MQ=255)
                                                                                                                                         cTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGAAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCg             <  5:19826/149‑1 (MQ=255)
                                                                                                                                          ttgaaTTGAGAGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCg             <  6:372835/145‑1 (MQ=255)
                                                                                                                                          ttCAATTGAGCGATCTACACCTTTACGGACAAAATATGATCCAACCTGAAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGTCGATAAATTTAACGGATGTTTGGGATTTGATCACTGTATTTTCTGTCCGGATAAATACCgg            >  7:333498/1‑149 (MQ=255)
                                                                                                                                            cAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCggg           <  7:3710/148‑1 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTATGGCCAAAATCTGTTACAAACTGCAGTAATAAGTTTTGTTGGTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGCTAAATACCGgga          <  3:24265/148‑1 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                                  <  2:232953/108‑1 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTgg                                                  >  1:232953/1‑108 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGggag         <  5:220532/149‑1 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGggag         <  7:187273/149‑1 (MQ=255)
                                                                                                                                             aaTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGgga          <  6:1198/148‑1 (MQ=255)
                                                                                                                                              aTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGggag         >  2:249256/1‑148 (MQ=255)
                                                                                                                                              aTTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGggag         >  6:23272/1‑148 (MQ=255)
                                                                                                                                                  aGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTc                                                                            <  8:289816/77‑1 (MQ=255)
                                                                                                                                                  aGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTc                                                                            >  7:289816/1‑77 (MQ=255)
                                                                                                                                                    cGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAAt  <  5:222042/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTGCGACCGGAGAAGAAATGCACATTGCTCCGCACGCCGAGTTTTTCTGCCAGCGCTTCAAATTTTCGCGGCTTATCCTGACCAACAACAAATAAAAGCGTATTGTGACGTAATGATTCCGGTAACGATGCCAAAGCTTCAATTGAGCGATCTACACCTTTACGGCCAAAATCTGATCCAACCTGCAGTAATAAGTTTTGTTGCTCTTTTATGCCATTTTTCTGGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAAT  >  NC_000913/3806282‑3806578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: