Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,448,424 T→A 5.2% intergenic (+189/‑23) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,4240TA 5.2% 701.0 / 17.3 211intergenic (+189/‑23)ytfP/chpSGGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (97/103);  new base A (6/5);  total (103/108)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 222 total aligned reads displayed.

GAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAT  >  NC_000913/4448276‑4448571
                                                                                                                                                    |                                                                                                                                                   
gagaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                                                     >  6:101431/1‑149 (MQ=255)
gagaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACtt                                                                                                                                                     >  4:130328/1‑149 (MQ=255)
 agaCGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACttt                                                                                                                                                    <  5:220638/149‑1 (MQ=255)
    cgacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGta                                                                                                                                                 <  8:341600/149‑1 (MQ=255)
     gacTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGta                                                                                                                                                 >  8:322573/1‑148 (MQ=255)
        tCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAAc                                                                                                                                             >  8:346446/1‑149 (MQ=255)
          gCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAAc                                                                                                                                             <  2:43700/147‑1 (MQ=255)
                  ttttGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAaggag                                                                                                                                     <  6:371207/147‑1 (MQ=255)
                          ttCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGc                                                                                                                           <  5:13875/149‑1 (MQ=255)
                          ttCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGc                                                                                                                           >  1:350466/1‑149 (MQ=255)
                              cTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAt                                                                                                                       >  1:119678/1‑149 (MQ=255)
                              cTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAt                                                                                                                       >  8:382839/1‑149 (MQ=255)
                              cTCACCTTTTGCCTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTAt                                                                                                                       >  5:284293/1‑149 (MQ=255)
                                cACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGATGGCATTCACAAATGCGCAGGGGTAACACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTa                                                                                                                     >  2:197277/1‑149 (MQ=255)
                                 aCCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTAc                                                                                                                    <  4:20589/149‑1 (MQ=255)
                                   cTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCa                                                                                                                  >  5:362086/1‑149 (MQ=255)
                                        gCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATaaa                                                                                                              >  4:333434/1‑148 (MQ=255)
                                         cTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTa                                                                                                                        >  1:186584/1‑137 (MQ=255)
                                           tttCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACAATaaa                                                                                                              >  3:264001/1‑145 (MQ=255)
                                            ttCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGAt                                                                                                         >  5:29972/1‑149 (MQ=255)
                                             tctctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGAt                                                                                                         <  7:346446/148‑1 (MQ=255)
                                              ctctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGa                                                                                                          <  5:250883/146‑1 (MQ=255)
                                               tctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATggg                                                                                                      >  3:34295/1‑149 (MQ=255)
                                               tctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                                       >  7:216277/1‑148 (MQ=255)
                                                ctcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                                       <  6:241459/147‑1 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGTCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTTGGACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATggg                                                                                                      <  6:71092/147‑1 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     >  6:4356/1‑148 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATggg                                                                                                      <  7:262405/147‑1 (MQ=255)
                                                 tcCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                                       <  6:16939/146‑1 (MQ=255)
                                                   cGAGCCGCTTTCCATATCTATTAACGGATAAAAAACTCTGCTGGCATTCACAAATGCGCATGGGTAAAACGTTTCCTGTTGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGaa                                                                                                  <  6:181340/149‑1 (MQ=255)
                                                   cGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     <  8:72203/146‑1 (MQ=255)
                                                   cGAGCCGCTTTCCATATCTATTAACACATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     <  7:17634/146‑1 (MQ=255)
                                                    gAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgg                                                                                                       >  8:225466/1‑143 (MQ=255)
                                                      gCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAg                                                                                               >  2:340468/1‑149 (MQ=255)
                                                        cGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTg                                                                                             >  7:323283/1‑149 (MQ=255)
                                                           tttCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAaggag                                                                                                                                     <  2:364570/106‑1 (MQ=255)
                                                             tCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAgg                                                                                         >  4:73842/1‑148 (MQ=255)
                                                                 tatCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATgg                                                                                    >  8:10426/1‑149 (MQ=255)
                                                                 tatCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATgg                                                                                    <  6:29972/149‑1 (MQ=255)
                                                                   tCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     >  7:322218/1‑130 (MQ=255)
                                                                   tCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGt                                                                                   <  3:73842/148‑1 (MQ=255)
                                                                   tCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGt                                                                                   >  3:193967/1‑148 (MQ=255)
                                                                     tATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGt                                                                                        >  4:3911/1‑141 (MQ=255)
                                                                     tATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCAt                                                                                >  1:60526/1‑149 (MQ=255)
                                                                      aTTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGt                                                                                                                                                  >  2:225681/1‑82 (MQ=255)
                                                                      aTTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGt                                                                                                                                                  <  1:225681/82‑1 (MQ=255)
                                                                      aTTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     >  7:75791/1‑127 (MQ=255)
                                                                       ttAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTc                                                                              <  2:374687/149‑1 (MQ=255)
                                                                         aaCGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTcc                                                                             <  1:302243/148‑1 (MQ=255)
                                                                          aCGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTccc                                                                            >  2:68066/1‑148 (MQ=255)
                                                                            gCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATgggg                                                                                                     >  7:66587/1‑121 (MQ=255)
                                                                            gCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCaa                                                                          >  3:122145/1‑148 (MQ=255)
                                                                                 aaaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCg                                                                     <  1:371047/148‑1 (MQ=255)
                                                                                 aaaaaCTCTGCTGGCATTCACAAATGAGCAGGGGTAAAAAGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGCAACAGTGCATGTATGGTCATTc                                                                              >  3:172792/1‑139 (MQ=255)
                                                                                  aaaaCTCTGCTGTCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTTAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTGTGGTCATTCCCAATATCGt                                                                    >  6:186454/1‑148 (MQ=255)
                                                                                  aaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATc                                                                      <  1:65893/146‑1 (MQ=255)
                                                                                  aaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGt                                                                    >  4:177142/1‑148 (MQ=255)
                                                                                  aaaaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGt                                                                    >  3:291523/1‑148 (MQ=255)
                                                                                    aaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAAt                                                                 <  1:41601/149‑1 (MQ=255)
                                                                                    aaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAAt                                                                 <  6:284293/149‑1 (MQ=255)
                                                                                    aaCTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAAt                                                                 <  6:156540/149‑1 (MQ=255)
                                                                                      ctctGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGa                                                               <  7:10426/149‑1 (MQ=255)
                                                                                       tctGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATACCCAATATCGTAATGa                                                               >  7:336815/1‑148 (MQ=255)
                                                                                           ctgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGaaa                                                             <  3:333434/146‑1 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAg                                                                                          >  5:177957/1‑116 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATgg                                                                                    <  4:240853/122‑1 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATg                                                                                     <  2:155494/121‑1 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaac                                                         >  8:159022/1‑149 (MQ=255)
                                                                                            tgGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATAAAAGaac                                                         >  4:273916/1‑149 (MQ=255)
                                                                                                      aaaTGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACa                                               <  2:58729/149‑1 (MQ=255)
                                                                                                           cgcAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCgg                                          <  2:61171/149‑1 (MQ=255)
                                                                                                             cAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGGACTTAACTTACAGCCgggg                                        <  5:113526/149‑1 (MQ=255)
                                                                                                                  gTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTg                                                                                                                                  <  1:138271/54‑1 (MQ=255)
                                                                                                                   tAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGc                                  <  5:160136/149‑1 (MQ=255)
                                                                                                                     aaaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGCACTTAACTTACAGCCGGGGCAGAGCGt                                >  5:317191/1‑149 (MQ=255)
                                                                                                                     aaaCGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                                <  5:62530/149‑1 (MQ=255)
                                                                                                                          tttCCTGTAGCACCGTGAGTTTTACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                               <  1:296677/145‑1 (MQ=255)
                                                                                                                          tttCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTGATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGCGCGTGGAgg                           >  3:21130/1‑149 (MQ=255)
                                                                                                                          tttCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                               >  2:296677/1‑145 (MQ=255)
                                                                                                                            tCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTGTGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGgc                          <  5:186454/148‑1 (MQ=255)
                                                                                                                              cTGTAGCTCCGTGAGTTATACTTTTTATAACTTAAGGAGGTTCAGATGCGTATTACCATAAATAGATGGGGGAACAGTGCAGGTTTGGTCATTCCCAATATCGTAATGAATGAACTTAACTTAct                                               <  7:153101/125‑2 (MQ=255)
                                                                                                                              cTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGgcgc                        >  4:160424/1‑148 (MQ=255)
                                                                                                                                 tAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGt                    <  1:68066/149‑1 (MQ=255)
                                                                                                                                  aGCACCGTGAGTTATACTTAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAAAAGTGGAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAAATTACAGCCGCGGCAGATCGAGGAGGCGCAAGt                    >  8:110491/1‑148 (MQ=255)
                                                                                                                                   gCACCGTGAGGTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGa                  <  4:291523/149‑1 (MQ=255)
                                                                                                                                     aCCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAg                 >  7:13975/1‑148 (MQ=255)
                                                                                                                                       cGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaa              >  8:262897/1‑149 (MQ=255)
                                                                                                                                        ttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaac                                                    >  5:338712/4‑110 (MQ=255)
                                                                                                                                        ttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                                >  2:238496/4‑130 (MQ=255)
                                                                                                                                        ttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGt                                <  1:238496/127‑1 (MQ=255)
                                                                                                                                        ttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaa              >  8:205905/4‑148 (MQ=255)
                                                                                                                                        ttaaGTTATACAAAGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGca               >  6:32323/4‑147 (MQ=255)
                                                                                                                                         tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGcaac             >  3:36019/1‑148 (MQ=255)
                                                                                                                                           aGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCg                                                                     >  6:86945/1‑90 (MQ=255)
                                                                                                                                               ataCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCaa       <  6:355789/148‑1 (MQ=255)
                                                                                                                                                taCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACt     <  5:4350/149‑1 (MQ=255)
                                                                                                                                                 aCTTTGTAAAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACt     >  5:327061/1‑148 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGaacttaac                                                    >  3:304978/1‑99 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                               >  2:87335/1‑120 (MQ=255)
                                                                                                                                                   tttGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTg                               <  1:87335/120‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGACGACTCGCATTCTGTTTTGTAATTCCCTCACCTTTTGCTTTTCTCTCCGAGCCGCTTTCCATATCTATTAACGCATAAAAAACTCTGCTGGCATTCACAAATGCGCAGGGGTAAAACGTTTCCTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAT  >  NC_000913/4448276‑4448571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: