Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,454,591 G→A 5.3% intergenic (+13/+20) yjfF → / ← fbp putative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,454,5910GA 5.3% 428.9 / 7.3 133intergenic (+13/+20)yjfF/fbpputative sugar ABC transporter permease/fructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base G (56/68);  new base A (2/5);  total (60/73)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 140 total aligned reads displayed.

GCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCT  >  NC_000913/4454443‑4454739
                                                                                                                                                    |                                                                                                                                                    
gCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCg                                                                                                                                                      >  5:161116/1‑149 (MQ=255)
  tGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGAAATAAAAACGCCTCTCCgtg                                                                                                                                                    >  6:113571/1‑149 (MQ=255)
    aGTTCCTGGTGGACGAATATCGCCATCGGTATGTTGTTGATTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCCGTGACAAGAGTCAACATTGCTCAGCAATAAAAACGCCTCTCCgtgt                                                                                                                                                   <  1:165250/148‑1 (MQ=255)
    aGTTCCTGGTGGACGAAAATCGCCATCGTTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCgtgt                                                                                                                                                   <  1:312157/148‑1 (MQ=255)
    aGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCgtgg                                                                                                                                                   <  5:259527/148‑2 (MQ=255)
    aGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGGGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                                  <  3:20388/149‑1 (MQ=255)
    aGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGAGTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCTAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCgtgt                                                                                                                                                   <  6:26047/148‑1 (MQ=255)
     gTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                                  <  5:362314/148‑1 (MQ=255)
     gTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTgg                                                                                                                                                 <  7:331592/149‑1 (MQ=255)
      atCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGga                                                                                                                                                <  2:39389/148‑1 (MQ=255)
             tggACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCACACGGAGAGgcgt                                                                                                                                         <  4:292721/149‑2 (MQ=255)
              ggACGAAAATCGCCCTCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCa                                                                                                                                        <  1:285772/149‑1 (MQ=255)
              ggACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCACACGGAGAGgcgtt                                                                                                                                        <  6:221092/149‑3 (MQ=255)
               gACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGga                                                                                                                                                <  8:301810/140‑1 (MQ=255)
                           cATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGt                                                                                                                           >  4:143923/1‑149 (MQ=255)
                           cATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGATGTGGGAGAATCGTCAGAGTACGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTATCCGTGTGGAGAGGCGCAGGAGATTACGCGt                                                                                                                           >  1:98455/1‑149 (MQ=255)
                             tCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                                  <  7:113360/124‑1 (MQ=255)
                             tCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGtgtg                                                                                                                                                  >  8:113360/1‑124 (MQ=255)
                                   ttttGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCTGGAAc                                                                                                                   <  8:167904/149‑1 (MQ=255)
                                    tttGTTGTTTATTTTTTTAGCATTACAGCGTGGAGTATCGGTGCTGTGGGAGAATCGTCAGAGTACGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAAc                                                                                                                   <  2:98455/148‑1 (MQ=255)
                                     ttgttgTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACt                                                                                                                  <  4:166132/148‑1 (MQ=255)
                                          gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGga                                                                                                                                                <  6:220449/113‑1 (MQ=255)
                                          gttTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGga                                                                                                                                                >  5:220449/1‑113 (MQ=255)
                                              aTTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATa                                                                                                          >  1:257822/1‑147 (MQ=255)
                                              aTTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCACACGGAGAGgc                                                                                                                                           >  3:210325/1‑114 (MQ=255)
                                              aTTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCACACGGAGAGgc                                                                                                                                           <  4:210325/114‑1 (MQ=255)
                                               tttttATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAg                                                                                                       <  3:143923/149‑1 (MQ=255)
                                               tttttATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAg                                                                                                       >  5:113817/1‑149 (MQ=255)
                                                    ataGAATTACAGCGTGTATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCGTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGtt                                                                                                   <  2:187440/148‑1 (MQ=255)
                                                       gCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGAc                                                                                               <  6:325172/149‑1 (MQ=255)
                                                          ttACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCTTATGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGAGAAAGCGGTCGACagc                                                                                            >  5:205625/1‑147 (MQ=255)
                                                              aGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTc                                                                                         >  3:116926/1‑148 (MQ=255)
                                                              aGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTc                                                                                         >  5:199374/1‑148 (MQ=255)
                                                                   ggATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCa                                                                                    >  4:342340/1‑148 (MQ=255)
                                                                     aTTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCatat                                                                                 <  6:132888/149‑1 (MQ=255)
                                                                      ttAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATTAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGTAACTCTCGGATAAAGCGTTCGACATCGTCAACCTTATg                                                                                >  2:261288/1‑149 (MQ=255)
                                                                      ttAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                                >  6:348912/1‑149 (MQ=255)
                                                                         aCGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCgg                                                                                                                       >  6:196913/1‑107 (MQ=255)
                                                                         aCGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCgg                                                                                                                       <  5:196913/107‑1 (MQ=255)
                                                                           ggTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGt                                                                           <  4:172017/149‑1 (MQ=255)
                                                                            gTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGtt                                                                          <  5:113571/149‑1 (MQ=255)
                                                                            gTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGtt                                                                          <  6:161116/149‑1 (MQ=255)
                                                                                 gtgGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGa                                                                     >  7:72435/1‑149 (MQ=255)
                                                                                       gaATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAg                                                                >  1:370666/1‑148 (MQ=255)
                                                                                        aaTCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGaa                                                              >  8:193338/1‑149 (MQ=255)
                                                                                            gTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGaa                                                              <  6:59733/145‑1 (MQ=255)
                                                                                            gTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGaa                                                          >  3:217406/1‑149 (MQ=255)
                                                                                             tCAGATTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGTAGAGGGGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAAc                                                         <  8:274140/149‑1 (MQ=255)
                                                                                             tCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAAc                                                         >  2:347836/1‑149 (MQ=255)
                                                                                             tCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAAc                                                         >  8:355871/1‑149 (MQ=255)
                                                                                                gagTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGgc                                                      >  1:117859/1‑149 (MQ=255)
                                                                                                  gTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGgcgc                                                    <  1:347836/149‑1 (MQ=255)
                                                                                                  gTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGgcgc                                                    <  7:355871/149‑1 (MQ=255)
                                                                                                       ccAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTg                                               >  1:99567/1‑149 (MQ=255)
                                                                                                          gTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGcc                                                                       <  1:129068/122‑1 (MQ=255)
                                                                                                          gTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGcc                                                                       >  2:129068/1‑122 (MQ=255)
                                                                                                           tGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAgg                                           <  6:199374/149‑1 (MQ=255)
                                                                                                           tGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAgg                                           <  1:189461/149‑1 (MQ=255)
                                                                                                           tGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAgg                                           <  1:69512/149‑1 (MQ=255)
                                                                                                           tGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAgg                                           <  5:70579/149‑1 (MQ=255)
                                                                                                             aCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACgg                                                       <  4:231579/135‑1 (MQ=255)
                                                                                                             aCAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGt                                         <  8:72435/149‑1 (MQ=255)
                                                                                                              cAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGtt                                        >  6:340620/1‑149 (MQ=255)
                                                                                                               aaGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACaaa                                                                 <  3:197395/123‑1 (MQ=255)
                                                                                                               aaGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACaaa                                                                 >  4:197395/1‑123 (MQ=255)
                                                                                                               aaGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGttt                                       >  2:385739/1‑149 (MQ=255)
                                                                                                                   gTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCgg                                   >  3:88890/1‑149 (MQ=255)
                                                                                                                    tCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTc                                      >  3:219773/1‑145 (MQ=255)
                                                                                                                    tCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTc                                      <  4:219773/145‑1 (MQ=255)
                                                                                                                      aaCATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                                >  2:351154/1‑101 (MQ=255)
                                                                                                                      aaCATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgat                                >  2:346945/1‑149 (MQ=255)
                                                                                                                      aaCATTGCGCAGCAATAAAAACGCCGCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgat                                <  7:75259/149‑1 (MQ=255)
                                                                                                                       aCATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAgg                                           >  3:332827/1‑137 (MQ=255)
                                                                                                                        cATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGgat                                <  5:264722/147‑1 (MQ=255)
                                                                                                                        cATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGACGACAAAGaa                                                              >  2:179218/1‑117 (MQ=255)
                                                                                                                             cgcAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATcc                         <  7:60021/149‑1 (MQ=255)
                                                                                                                             cgcAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATc                          <  4:88890/148‑1 (MQ=255)
                                                                                                                              gcagcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTgg                                                 >  5:174858/1‑124 (MQ=255)
                                                                                                                              gcagcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTgg                                                 <  6:174858/124‑1 (MQ=255)
                                                                                                                              gcagcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCa                        <  2:257822/149‑1 (MQ=255)
                                                                                                                                 gcaATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGaa                     <  3:342340/149‑1 (MQ=255)
                                                                                                                                     tAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATaaa                                                                                                        >  3:178658/1‑62 (MQ=255)
                                                                                                                                       aaaaCGCCTCTCCGTGTTGAGAGGCTGAGGAGATTACGCGTCCGTGAACTCAGGGATAAAGCGTTCGACATGTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGc                <  1:81466/148‑1 (MQ=255)
                                                                                                                                         tgcGCCTCTCCACACGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTc                                                                                                  >  5:77402/3‑64 (MQ=25)
                                                                                                                                           cGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCttt           <  7:193338/149‑1 (MQ=255)
                                                                                                                                           cGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCttt           <  1:346945/149‑1 (MQ=255)
                                                                                                                                           cGCCTCTCCACACGGAGAGGCGTAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCttt           <  4:217891/149‑1 (MQ=255)
                                                                                                                                              ctctCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTc                                                                             >  6:291929/1‑80 (MQ=255)
                                                                                                                                              ctctCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTc                                                                             <  8:7844/80‑1 (MQ=255)
                                                                                                                                               tctcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATg                                                                                >  3:287079/1‑76 (MQ=255)
                                                                                                                                               tctcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCa       >  5:40133/1‑149 (MQ=255)
                                                                                                                                               tctcCGTGTGGAGAGGCGCAGGAGATTACGCGACCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCa       >  6:16448/1‑149 (MQ=255)
                                                                                                                                                ctcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATATTCAACCATATGGTCGTTTCCGACAAAGAATGAACGGCGCTCGTGCAGGGTTTCCGCGAAGATATCCAGAAAACGCTCTTTGCCa       >  4:318249/1‑148 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGTGGCGCAGGAGATTACGCGTCCGGGATCTCACGGATTAAGCGTTCGACATCTTCAACCATTTGGTCGTTTCCGACTAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCttt           <  3:71804/143‑1 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCa       <  2:99567/147‑1 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtc     >  6:119136/1‑149 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtc     >  8:213818/1‑149 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtc     >  1:31011/1‑149 (MQ=255)
                                                                                                                                                 tcCGTGTGGAGAGGCGCAGGAGATTACGCGTACGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGAAATCCAGAATACGcagttt           >  4:71804/1‑138 (MQ=255)
                                                                                                                                                    gtgtGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCAtcgct  >  8:108315/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGAGTTCCTGGTGGACGAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAGCAATAAAAACGCCTCTCCGTGTGGAGAGGCGCAGGAGATTACGCGTCCGGGAACTCACGGATAAAGCGTTCGACATCTTCAACCATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCT  >  NC_000913/4454443‑4454739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: