Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 696,952 T→C 5.2% intergenic (‑13/+11) glnU ← / ← leuW tRNA‑Gln/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,9520TC 5.2% 238.4 / 2.7 77intergenic (‑13/+11)glnU/leuWtRNA‑Gln/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (44/29);  new base C (2/2);  total (46/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696810‑697098
                                                                                                                                              |                                                                                                                                                  
ttACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGCTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTTGCGATACCCACTCCGTACAACGCTTTCTgg                                                                                                                                              >  7:51120/1‑149 (MQ=255)
ttACCGCTTGGCGATACCCCAAAAACAGGGCGGTTAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATACGAACCAGGCACAACCTCGATAAACAGGAGGTGCCTAACCGCTTGGCGATCCCCCATCAGAAGAACGATTTCTgg                                                                                                                                              >  4:107306/1‑149 (MQ=255)
 tACCGCTTGGCGATACCCCAATACCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGCACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCACATCCGTACAACGCTTTCTGGt                                                                                                                                             >  3:267917/1‑149 (MQ=255)
   ccGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCATGGCGAAACCCCATCCGTACACCGCTTTCTGGTg                                                                                                                                            >  1:244005/1‑148 (MQ=255)
                 cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGc                                                                                                                             >  1:110188/1‑149 (MQ=255)
                   cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTTTCAAACACCGGTGCCTTACCGCTTGGCGATACCCCCTCCGTACACCGCTGTCTGGTGAATGGTGCGGGAGGCgt                                                                                                                           >  8:139183/1‑148 (MQ=25)
                   cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                                                                                                                                             <  2:231949/131‑1 (MQ=255)
                   cAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt                                                                                                                                             >  1:231949/1‑131 (MQ=255)
                           gggCGTTGAACCGCTTACTGGAAGAAGATGGCTGGGGTACCTTGATTCGAACCATGGAATGCCGGTATGAAAAACCGTTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGaa                                                                                                                   <  8:51120/149‑1 (MQ=25)
                               ggTGAACCGCTTAATCGAAGAAGATGGCGGGGGTACATGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATAACCCTTGCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCg                                                                                                               <  2:110188/149‑1 (MQ=25)
                                     ccGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACAcc                                                                                                         >  6:324651/1‑149 (MQ=255)
                                             tCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggcg                                                                                                 >  4:149018/1‑149 (MQ=255)
                                               gaagaaGATGGGTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGc                                                                                                                                    >  5:260141/1‑112 (MQ=255)
                                               gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGc                                                                                                                                    <  6:260141/112‑1 (MQ=255)
                                               gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  8:308709/1‑148 (MQ=255)
                                               gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  1:348782/1‑148 (MQ=255)
                                               gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  8:69077/1‑148 (MQ=255)
                                               gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  3:335120/1‑148 (MQ=255)
                                                aagaagATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGgcgc                                                                                                >  7:328810/1‑147 (MQ=255)
                                                  gaagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAg                                                                                             >  5:240747/1‑148 (MQ=255)
                                                   acgaTGGCGTGGGTACCTGGATTCGAACCAGTGAATGCCTGCATCAAACACCGGTGACTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGaa                                                                                           <  2:244005/147‑1 (MQ=37)
                                                   aagaTGGCGTGGGTACCTGTATTCGACCCAGGTAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGaa                                                                                           <  4:267917/149‑1 (MQ=37)
                                                     gaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                                                                                                                                                <  5:95890/94‑1 (MQ=255)
                                                     gaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt                                                                                                                                                >  6:95890/1‑94 (MQ=255)
                                                                   cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGt                                                                           >  7:87741/1‑149 (MQ=255)
                                                                   cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTCGTGCGt                                                                           >  2:183926/1‑149 (MQ=255)
                                                                   cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCCCTTGCCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCTCGACTTGAACTCGCACACCATGCGGCGCCAGAACCTAAATCTGGTGctt                                                                           >  5:279033/1‑147 (MQ=37)
                                                                       aTTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGAGCGTCTAc                                                                       >  7:315128/1‑149 (MQ=255)
                                                                          cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgc                                                                                                     >  2:387548/1‑116 (MQ=255)
                                                                          cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgc                                                                                                     <  1:387548/116‑1 (MQ=255)
                                                                           gAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAAt                                                                   >  4:223461/1‑149 (MQ=255)
                                                                               cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt                                                                                                                                      <  1:364365/78‑1 (MQ=255)
                                                                               cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCg                                                               >  6:33620/1‑149 (MQ=255)
                                                                               cAGGGAATGCCGGTATCAAAAACCGGTGCATTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt                                                                                                                                      >  2:364365/1‑78 (MQ=255)
                                                                                aGGGATCGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGc                                                              <  3:181883/149‑1 (MQ=255)
                                                                                  ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggc                                                                                                  >  1:222986/1‑111 (MQ=255)
                                                                                  ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTgcggc                                                                                                  <  2:222986/111‑1 (MQ=255)
                                                                                    aaTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTTGTGCGTCTACCAATTTCGCAACt                                                          >  6:336864/1‑149 (MQ=255)
                                                                                    aaTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACt                                                          >  6:342297/1‑149 (MQ=255)
                                                                                     aTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTc                                                         <  5:342297/149‑1 (MQ=255)
                                                                                     aTGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCCTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATATCGCCACTc                                                         <  5:336864/149‑1 (MQ=255)
                                                                                         cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCAGGACAACGCTGTCTGGGGAATGGTGCGGGAGGCGAGGCGTGAACTCGCACCCCTAGCGGCGCCAGCACGTAAATCAGCGGCGTCGAGCAAGATAGCCAcatcgg                                                      >  4:181883/1‑143 (MQ=19)
                                                                                           gTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCa                                                    >  6:140294/1‑148 (MQ=255)
                                                                                              tCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaa                                                 >  4:70499/1‑148 (MQ=255)
                                                                                                aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGAc                                                                                                                        <  4:106352/75‑1 (MQ=255)
                                                                                                aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAACTCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaa                                                >  3:206059/1‑147 (MQ=255)
                                                                                                aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaaaa                                              <  8:328810/149‑1 (MQ=255)
                                                                                                aaaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaaaa                                              >  1:183026/1‑149 (MQ=255)
                                                                                                aaaaaCCGGTGCCTTACCGCTTGGAGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGAc                                                                                                                        >  3:106352/1‑75 (MQ=255)
                                                                                                aaaaaCCGGTGCCGTACCGCTTGGCGATACCCCATCCTTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACATGAACTCGCACACCTTGCGGCGCCAGAACCTAACTCTGGTGCGTCTACCAATTTCGCCACTCCCGCaaaaa                                                >  3:281166/1‑147 (MQ=255)
                                                                                                 aaaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAg                                             <  5:33620/149‑1 (MQ=255)
                                                                                                    aCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtg                                          <  2:348782/149‑1 (MQ=255)
                                                                                                     ccGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtgg                                         <  5:140294/149‑1 (MQ=255)
                                                                                                       ggTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtggtg                                       <  6:240747/149‑1 (MQ=255)
                                                                                                        gTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGAtggtgg                                      <  2:183026/149‑1 (MQ=255)
                                                                                                                  gCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACgg                             >  1:336531/1‑148 (MQ=255)
                                                                                                                         gATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGAGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGaa                     <  8:315128/149‑1 (MQ=255)
                                                                                                                          aTACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCACAAAAAAAGATGGTGGCTACGACTGGATTCGAAc                    >  1:338802/1‑149 (MQ=255)
                                                                                                                          aTACACCATCCGTACAACGCTTTCTGGTGAATGGTGCGTGAGGCTAGTCTTGAACTCTCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTGCTTCATTAAAAGATGGTGGCTACGACGGGATTCGAAc                    <  2:72288/149‑1 (MQ=255)
                                                                                                                             ccccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtg                 <  3:70499/149‑1 (MQ=255)
                                                                                                                              cccATCCGTACACCGCTTTCTGGTGAGTGGTGCGGGAGGCGAGCCTTGAACTCGCCCCCATTGCGGCGCCAGACCCTAAATCTGGTGCGTCTACCATTTTCGCCCATCCCGCAAAAAAAGATGGTGCCTCCGCCGGGATTCGACCCtgt                >  4:179677/1‑149 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             >  7:240972/1‑104 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             <  8:240972/104‑1 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             <  4:172784/104‑1 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGcc                                                             >  3:172784/1‑104 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  5:324651/149‑1 (MQ=255)
                                                                                                                              cccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  8:87741/149‑1 (MQ=255)
                                                                                                                               ccATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                >  3:221155/1‑148 (MQ=255)
                                                                                                                                cATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAtt                         <  6:116751/138‑1 (MQ=255)
                                                                                                                                cATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAtt                         >  5:116751/1‑138 (MQ=255)
                                                                                                                                 aTCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAc             <  3:107306/149‑1 (MQ=255)
                                                                                                                                  tCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAcc            <  7:69077/149‑1 (MQ=255)
                                                                                                                                    cGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGAccc           >  7:38363/1‑148 (MQ=255)
                                                                                                                                        cAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCcatca      >  8:306030/1‑149 (MQ=255)
                                                                                                                                         aaCGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCcatca      >  1:195701/1‑148 (MQ=255)
                                                                                                                                            gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTGGAACCTGTGACCCCATCATTAt  <  2:336531/149‑1 (MQ=255)
                                                                                                                                            gCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCGACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGAGTCGAACCTGTGACCCCATCATTAt  >  2:260715/1‑149 (MQ=255)
                                                                                                                                              tttCTGTTGAATTGTGCGGGAGGCGAGTCTTCTTGTCGCAGACCTTGCGGCGCAAGATCCTAAATCTGGTGCGTCTTCCTATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACTACGGGATTCGATCGTGTGTCGCCATCATTAt  <  6:101717/147‑1 (MQ=255)
                                                                                                                                              tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                >  8:240914/1‑133 (MQ=255)
                                                                                                                                              tttCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCtgt                <  7:240914/133‑1 (MQ=255)
                                                                                                                                              tttCTGGTGAATGGTGAGGGAGGCGAGACTTGAACTCGCACACCTTGCGTCGCCAGAACCTAAATCTGGTGCTTCTACCAATTTCGCCACTCCCGCTAAAAAAGATGCTGGCTACGACGGGATTCGAACCTGTGACCCAATCAatat  >  5:101717/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTAT  >  NC_000913/696810‑697098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: