Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	745,590	C→T	5.2%	S142S (AGC→AGT)	ybgL →	UPF0271 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	745,590	0	C	T	5.2%	247.2 / 2.6	76	S142S (AGC→AGT)	ybgL	UPF0271 family protein
Reads supporting (aligned to +/- strand):  ref base C (34/38);  new base T (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAG  >  NC_000913/745442‑745722                                                                                                                                                     |                                                                                                                                     cccGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGc                                                                                                                                      <  6:5737/149‑1 (MQ=255)        gcAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCt                                                                                                                                 >  7:107072/1‑147 (MQ=255)         cAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGAt                                                                                                                              <  3:150185/149‑1 (MQ=255)               ggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTg                                                                                                                         >  2:9554/1‑148 (MQ=255)               ggcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGc                                                                                                                        <  8:240991/149‑1 (MQ=255)                gcgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCa                                                                                                                       <  7:5961/149‑1 (MQ=255)                 cgTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGACGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAg                                                                                                                      <  1:91027/149‑1 (MQ=255)                     aTGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGgcaa                                                                                                                  <  8:107072/149‑1 (MQ=255)                          tCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGTGAGCTGATTCGTGc                                                                                                                        <  8:272237/138‑1 (MQ=255)                          tCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGTGAGCTGATTCGTGc                                                                                                                        >  7:272237/1‑138 (MQ=255)                          tCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGgcaagcaa                                                                                                              >  1:19100/1‑148 (MQ=255)                                 aaaCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCCAGCAATATGGTCt                                                                                                      >  4:319015/1‑149 (MQ=255)                                 aaaCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCt                                                                                                      <  6:327610/149‑1 (MQ=255)                                 aaaCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCt                                                                                                      >  5:327610/1‑149 (MQ=255)                                   aCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAg                                                                                                                                   >  7:222051/1‑118 (MQ=255)                                   aCCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAg                                                                                                                                   <  8:222051/118‑1 (MQ=255)                                       cACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAc                                                                                                >  3:166106/1‑149 (MQ=255)                                       cACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCAGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAc                                                                                                <  2:77780/149‑1 (MQ=255)                                         cGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAcgc                                                                                              <  5:126568/149‑1 (MQ=255)                                          ggCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAcgcg                                                                                             >  7:241823/1‑149 (MQ=255)                                              tgttgtACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCt                                                                                                                                 >  8:287666/1‑109 (MQ=255)                                              tgttgtACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCt                                                                                                                                 <  7:287666/109‑1 (MQ=255)                                                  gtACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGaa                                                                                      <  8:295832/148‑1 (MQ=255)                                                   tACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAgt                                                                                    <  1:231346/149‑1 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAcgcgc                                                                                            >  2:218760/1‑136 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAAcgcgc                                                                                            <  1:218760/136‑1 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTg                                                                               >  1:35654/1‑149 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTg                                                                               >  8:369922/1‑149 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTg                                                                               >  5:127759/1‑149 (MQ=255)                                                        ccAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTg                                                                               <  1:63715/149‑1 (MQ=255)                                                         cAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGc                                                                              <  1:9554/149‑1 (MQ=255)                                                                 gAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                      >  1:214730/1‑149 (MQ=255)                                                                 gAAAGAAGCACAACTGGCAGAAGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGAAAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                      >  5:238806/1‑149 (MQ=255)                                                                    agaagCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGa                                                                                          >  3:315615/1‑126 (MQ=255)                                                                    agaagCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGa                                                                                          <  4:315615/126‑1 (MQ=255)                                                                     gaagCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTGTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTa                                                                  <  2:372345/149‑1 (MQ=255)                                                                      aagCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGc                                                                                                                        >  6:23348/1‑94 (MQ=255)                                                                      aagCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGc                                                                                                                        <  5:23348/94‑1 (MQ=255)                                                                        gCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCa                                                               <  2:19100/149‑1 (MQ=255)                                                                         cacaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAg                                                              <  8:241823/149‑1 (MQ=255)                                                                          acaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGTGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                      >  1:195572/1‑140 (MQ=255)                                                                          acaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGTGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATcgcg                                                                      <  2:195572/140‑1 (MQ=255)                                                                          acaACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAgg                                                             >  5:314108/1‑149 (MQ=255)                                                                           caACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGc                                                            >  3:160964/1‑149 (MQ=255)                                                                              cTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGa                                                         <  4:166106/149‑1 (MQ=255)                                                                               tGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGAc                                                        >  1:122297/1‑149 (MQ=255)                                                                               tGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGAc                                                        >  1:350138/1‑149 (MQ=255)                                                                                 gCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACgg                                                      >  2:85302/1‑149 (MQ=255)                                                                                              ccAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcg                                         >  2:281868/1‑149 (MQ=255)                                                                                               cAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcgc                                        >  7:200428/1‑149 (MQ=255)                                                                                                  agCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGaa                                     <  2:350138/149‑1 (MQ=255)                                                                                                  agCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGaa                                     <  4:160964/149‑1 (MQ=255)                                                                                                   gCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAg                                    >  1:94544/1‑149 (MQ=255)                                                                                                       tataCGCTTGCGATCCATCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGTTCTGACAACGCGCGAGGAAGTGTATGCCGATCGCGGTTATCAGGCTGACGGCTCGATGGTGCAGCGCAGCCAg                                >  2:382572/1‑149 (MQ=255)                                                                                                         taCGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGt                               <  2:94544/148‑1 (MQ=255)                                                                                                         taCGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTc                              <  8:200428/149‑1 (MQ=255)                                                                                                           cgcgTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAg                            <  1:382572/149‑1 (MQ=255)                                                                                                              ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAg                                                              >  7:93375/1‑112 (MQ=255)                                                                                                              ttGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAg                                                              <  8:93375/112‑1 (MQ=255)                                                                                                                  gATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGtt                     <  6:314108/149‑1 (MQ=255)                                                                                                                      cAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGttgatt                 <  6:238806/149‑1 (MQ=255)                                                                                                                      cAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGttgat                  <  7:369922/148‑1 (MQ=255)                                                                                                                        gCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGc                                                 <  5:329629/115‑1 (MQ=255)                                                                                                                        gCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGc                                                 >  6:329629/1‑115 (MQ=255)                                                                                                                         cATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGttgattga               >  8:70508/1‑148 (MQ=255)                                                                                                                         cATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGGGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaa              <  6:206956/149‑1 (MQ=255)                                                                                                                           ttGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaaaa            <  2:35654/149‑1 (MQ=255)                                                                                                                           ttGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGAGCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGaaaa            <  2:122297/149‑1 (MQ=255)                                                                                                                             gATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAAc           >  3:330535/1‑148 (MQ=255)                                                                                                                              aTTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGttga                   >  1:258006/1‑139 (MQ=255)                                                                                                                              aTTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGttga                   <  2:258006/139‑1 (MQ=255)                                                                                                                               ttCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACgaa        <  6:127759/149‑1 (MQ=255)                                                                                                                                tctcGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACg          >  7:152128/1‑146 (MQ=255)                                                                                                                                    gtcgGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGAAGGCTCTCTGGTGACGAGAAGCCAGTCAGGCGCGTTGATTGAAAACgaagaa     >  3:71516/1‑147 (MQ=255)                                                                                                                                     tcgGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAg  <  1:85302/149‑1 (MQ=255)                                                                                                                                      cgGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcg                                         >  5:113501/1‑109 (MQ=255)                                                                                                                                      cgGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCcg                                         <  6:113501/109‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                     CCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCAGACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCGGGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTTGCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTGATTGAAAACGAAGAACAG  >  NC_000913/745442‑745722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑