Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 839,232 T→C 6.0% intergenic (‑25/+17) ybiX ← / ← fiu Fe(II)‑dependent oxygenase superfamily protein/catecholate siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913839,2320TC 6.0% 204.9 / 3.0 67intergenic (‑25/+17)ybiX/fiuFe(II)‑dependent oxygenase superfamily protein/catecholate siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base C (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

TCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGA  >  NC_000913/839084‑839378
                                                                                                                                                    |                                                                                                                                                  
tCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCt                                                                                                                                                    >  6:246269/1‑149 (MQ=255)
 cTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCt                                                                                                                                                    <  8:178233/148‑1 (MQ=255)
  ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgc                                                                                                                                                  >  2:367545/1‑149 (MQ=255)
    aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACGCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGCCGTCCTCTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgcgc                                                                                                                                                >  5:276741/1‑149 (MQ=255)
       ttGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGGATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGccc                                                                                                                                              >  5:134764/1‑148 (MQ=255)
           gcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACAt                                                                                                                                         >  4:309922/1‑149 (MQ=255)
            cACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGGGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATc                                                                                                                                        >  6:105545/1‑149 (MQ=255)
             aCCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCt                                                                                                                                       >  7:31836/1‑149 (MQ=255)
             aCCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCt                                                                                                                                       >  3:293571/1‑149 (MQ=255)
              ccTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCt                                                                                                                                       >  1:5747/1‑148 (MQ=255)
                 ggtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAAt                                                                                                                                   <  8:74968/148‑1 (MQ=255)
                   tgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCa                                                                                                                                 <  6:207200/149‑1 (MQ=255)
                     cgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGa                                                                                                                               <  5:105545/149‑1 (MQ=255)
                     cgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGa                                                                                                                               <  7:309652/149‑1 (MQ=255)
                     cgcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGa                                                                                                                               <  2:303293/149‑1 (MQ=255)
                          gtggtgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGc                                                                                                                          <  3:115375/149‑1 (MQ=255)
                             gtgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCata                                                                                                                       >  1:217213/1‑149 (MQ=255)
                              tgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCatat                                                                                                                      >  8:332843/1‑149 (MQ=255)
                               gACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATAtt                                                                                                                     >  7:188547/1‑149 (MQ=255)
                                aCGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTg                                                                                                                    >  2:134497/1‑149 (MQ=255)
                                 cgcgTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTgg                                                                                                                   <  1:367545/149‑1 (MQ=255)
                                   cgTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCt                                                                                                                 <  3:309922/149‑1 (MQ=255)
                                       catccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGa                                                                                                             <  8:31836/149‑1 (MQ=255)
                                           caCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCaa                                                                                                         <  4:293571/149‑1 (MQ=255)
                                           caCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAACGCATATTGGCTGTGAGCaa                                                                                                         <  6:283330/149‑1 (MQ=255)
                                             cccATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGTTGTGAGCaag                                                                                                        >  1:51223/1‑148 (MQ=255)
                                             cccATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCaag                                                                                                        >  7:196188/1‑148 (MQ=255)
                                              ccATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCaag                                                                                                        >  2:25074/1‑147 (MQ=255)
                                                     gCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCtt                                                                                               <  2:5747/149‑1 (MQ=255)
                                                             cAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGcc                                                                                       >  1:137227/1‑149 (MQ=255)
                                                                   ttCGCGAAAACGAGCTACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGt                                                                                  >  4:235281/1‑148 (MQ=255)
                                                                   ttCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATGGGAAACAGAATTGCATTTTGGGTTTCAGCAAGAAGTTAATTGGCTCGCCCgggg                                                                                  >  1:262327/1‑147 (MQ=255)
                                                                      gcgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGAt                                                                               >  4:123687/1‑148 (MQ=255)
                                                                       cgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATaa                                                                             <  5:246269/149‑1 (MQ=255)
                                                                            aagAGCGACGTCCTGTGGCGATAAGAGGCGGGGAATGTTGTAGATCTTAGATTTTCTCCAAAAAGTTGGGGCTGCGGATCACTTCTGAATCTGTATTGCATATTGGCTGTGAGCTAGAAGGTTCTTGGCTCGCCAGGGTGATACCGGTa                                                                        <  6:276741/147‑1 (MQ=255)
                                                                            aCGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTa                                                                        <  2:137227/149‑1 (MQ=255)
                                                                                   aCGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGTGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGctc                                                                 <  4:254450/149‑1 (MQ=255)
                                                                                    cGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGctc                                                                 <  3:235281/148‑1 (MQ=255)
                                                                                      tCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCttg                                                              <  2:230515/149‑1 (MQ=255)
                                                                                        cTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCttgt                                                             <  1:25074/148‑1 (MQ=255)
                                                                                             gCGATAACACGCCGGGAATGTGGTACATCATACATTTTCTCCAAAATGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCCTATTGTCTGTGAGCAAGACGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGttgatt                                                        <  6:134764/148‑1 (MQ=255)
                                                                                               gATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAg                                                     >  5:95319/1‑149 (MQ=255)
                                                                                               gATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAg                                                     <  7:332843/149‑1 (MQ=255)
                                                                                               gATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAg                                                     <  1:134497/149‑1 (MQ=255)
                                                                                                    cacGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGAGAc                                                >  8:28035/1‑149 (MQ=255)
                                                                                                     acGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGt                                                                                  >  5:48031/1‑114 (MQ=255)
                                                                                                     acGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGt                                                                                  <  6:48031/114‑1 (MQ=255)
                                                                                                     acGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCCGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACg                                               >  5:8328/1‑149 (MQ=255)
                                                                                                         cGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGTTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                           <  2:51223/149‑1 (MQ=255)
                                                                                                           ggAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCg                                         >  8:219052/1‑149 (MQ=255)
                                                                                                              aTGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTa                                      <  8:196188/149‑1 (MQ=255)
                                                                                                                   gTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaaa                                 <  2:217213/149‑1 (MQ=255)
                                                                                                                     aCATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGtt                                                                                                  <  2:351829/82‑1 (MQ=255)
                                                                                                                     aCATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGtt                                                                                                  >  1:351829/1‑82 (MQ=255)
                                                                                                                          atACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGtt                           <  8:188547/148‑1 (MQ=255)
                                                                                                                          atACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTg                          >  1:163305/1‑149 (MQ=255)
                                                                                                                           tACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTg                          >  1:320617/1‑148 (MQ=255)
                                                                                                                              aaTTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTaaa                      >  6:86699/1‑149 (MQ=255)
                                                                                                                               aTTTCTCCAAAAAGTGGGGCCCGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAAc                     <  6:8328/149‑1 (MQ=255)
                                                                                                                                   ctcCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGtt                  <  5:86699/148‑1 (MQ=255)
                                                                                                                                   ctcCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGtt                  >  5:227456/1‑148 (MQ=255)
                                                                                                                                     ccAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAg               >  1:78/1‑149 (MQ=255)
                                                                                                                                      cAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGttt                 <  3:123687/146‑1 (MQ=255)
                                                                                                                                      cAAAAAGTGGGGCCCGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGc              >  6:232409/1‑149 (MQ=255)
                                                                                                                                       aaaaaGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCt             >  7:78612/1‑149 (MQ=255)
                                                                                                                                           aGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGaa         <  2:320617/149‑1 (MQ=255)
                                                                                                                                           aGTGGGGCCCGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGaa         <  5:232409/149‑1 (MQ=255)
                                                                                                                                                  ccTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGTGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCgaga  <  2:163305/149‑1 (MQ=255)
                                                                                                                                                  ccTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGGGAGCAAGAAGGGTCTTGGCTCGCCCGGGTTATAACGGTAGCCGCGCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAACCGTTTAGCTGGAAGTCgaga  <  7:28035/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGA  >  NC_000913/839084‑839378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: