| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 909,353 | G→A | 74.0% | A566V (GCG→GTG) | poxB ← | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 909,353 | 0 | G | A | 74.0% | 86.3 / 34.1 | 56 | A566V (GCG→GTG) | poxB | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (19/22); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
GTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCAC > NC_000913/909205‑909483 | gTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCg < 3:221410/149‑1 (MQ=255) cAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACt > 8:153248/1‑149 (MQ=255) aaCCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCAGCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACtt > 1:330640/1‑149 (MQ=255) aCCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACtt > 6:182070/1‑148 (MQ=255) aCCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTtc < 6:298615/149‑1 (MQ=255) atcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGc < 2:194768/148‑1 (MQ=255) gCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgat < 5:182070/149‑1 (MQ=255) ttCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGT‑CGCTgatgat > 5:336176/1‑149 (MQ=255) gAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGT‑CGCTGATGATTgc < 6:336176/149‑1 (MQ=255) gcgcGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCATTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTAATCACCGCGTCCGCTGATGATTCCGCgcag > 6:217236/1‑149 (MQ=255) cgGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCat < 8:364369/149‑1 (MQ=255) cTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCAtata > 1:289126/1‑149 (MQ=255) gTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAg < 1:12095/148‑1 (MQ=255) ggTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGa < 2:289126/149‑1 (MQ=255) ggTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGAAGATTGCGCGCAGCATATACAGGCTg > 7:356859/1‑148 (MQ=255) aaCGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgatg < 3:273775/121‑1 (MQ=255) aaCGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgatg > 4:273775/1‑121 (MQ=255) tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCTCCCTTTTTACCTTAGCCATTTTGTTTTCAACAGTTCGATCACTTCATCACCGCGTCCGCTTATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg < 8:138936/149‑1 (MQ=255) tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg < 7:153248/149‑1 (MQ=255) tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg < 5:131408/149‑1 (MQ=255) aCTGCGTAAATCAATCATGGCATGTCCTTATTATTACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATAACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCt > 6:198267/1‑149 (MQ=255) cTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCatat < 1:219049/127‑1 (MQ=255) cTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCatat > 2:219049/1‑127 (MQ=255) aaaTCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATc < 7:24067/81‑1 (MQ=255) aaaTCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATc > 8:24067/1‑81 (MQ=255) aaaTCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCAAGTTTGTTTTCTCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAg > 7:295285/1‑149 (MQ=255) atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACt < 1:313468/82‑1 (MQ=255) atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACt > 2:313468/1‑82 (MQ=255) atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt > 3:81980/1‑149 (MQ=255) caatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGttt > 7:338907/1‑148 (MQ=255) tcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATc > 4:242938/1‑149 (MQ=255) tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGttt < 8:148699/142‑1 (MQ=255) tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGttt > 7:148699/1‑142 (MQ=255) tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGAtt < 4:179752/98‑1 (MQ=255) tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGAtt > 3:179752/1‑98 (MQ=255) aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg > 7:313210/1‑149 (MQ=255) aTGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg < 3:242938/149‑1 (MQ=255) tCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCGGATGATTGCGCGCAGCATATACAGGCTTAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGaa < 5:185892/149‑1 (MQ=255) cTTATTATGACGGGAAATGCCACCCTTTTTACATTAGCCAGTTTGTTTTCACCAGTTCGAACACTTAAACACCGCGACCGCTGATGATTCCGCGCAGCATAAACACGCTGACACCTTTGGCCTGTTCGAGTTTGATCTGCGTTGGAATg > 4:310074/1‑149 (MQ=255) ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATgg > 4:343557/1‑149 (MQ=255) attatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgca > 4:294781/1‑93 (MQ=255) attatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgca < 3:294781/93‑1 (MQ=255) attatGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc < 8:356859/148‑1 (MQ=255) aCGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAACCCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAActc < 4:7723/148‑1 (MQ=255) cGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg < 2:336336/134‑1 (MQ=255) aaaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCttt < 8:313210/149‑1 (MQ=255) aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg < 4:81980/149‑1 (MQ=255) ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt > 7:49715/1‑129 (MQ=255) ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt > 7:55680/1‑129 (MQ=255) ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt < 8:49715/129‑1 (MQ=255) ccACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAAt < 8:55680/129‑1 (MQ=255) cACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac > 5:279790/1‑149 (MQ=255) cACCCTTATTACCTTAGACAGTTTCTTTTCACCAGTTCGATCACTTCATCACCGCGTCAGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGac > 6:358898/1‑149 (MQ=255) ccTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACa < 2:386823/82‑1 (MQ=255) cTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGACCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccac < 3:343557/149‑1 (MQ=255) aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttct < 2:86379/126‑1 (MQ=255) aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttct > 1:86379/1‑126 (MQ=255) | GTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCAC > NC_000913/909205‑909483 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |