Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,151	(A)6→5	intergenic (‑125/‑237)	coaA ← / → thrU	pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,146	0	A	.	100.0%	105.7 / NA	24	intergenic (‑120/‑242)	coaA/thrU	pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/11);  total (13/11)

TCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTG  >  NC_000913/4175023‑4175272                                                                                                                            |                                                                                                                               tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTa                                                                                                          >  6:64010/1‑145 (MQ=255) tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTa                                                                                                          <  5:64010/145‑1 (MQ=255)                                             tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                     >  1:14871/1‑106 (MQ=255)                                             tatTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGtt                                                                                                     <  2:14871/106‑1 (MQ=255)                                                             tCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAgcg                                           >  4:16275/1‑148 (MQ=255)                                                               acaGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAt                                        >  3:57442/1‑149 (MQ=255)                                                                     cTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCAttgttg                                   <  3:16275/148‑1 (MQ=255)                                                                       gTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                 >  3:123741/1‑132 (MQ=255)                                                                       gTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCa                                                 <  4:123741/132‑1 (MQ=255)                                                                       gTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                  >  3:113715/1‑147 (MQ=255)                                                                       gTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGa                                  <  4:113715/147‑1 (MQ=255)                                                                          gAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑GAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCGTTGTTGAGcac                              >  1:48429/1‑148 (MQ=255)                                                                           aaTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                         <  2:68404/120‑1 (MQ=255)                                                                           aaTGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                         >  1:68404/1‑120 (MQ=255)                                                                               ggTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatgat                        >  2:124864/1‑149 (MQ=255)                                                                                          aggaggGTAACACCAGATGGGGAGGAATAATAAG‑GAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCGTTGTTGAGCACAATGATGTTGAAAAAgt             <  2:48429/149‑1 (MQ=255)                                                                                           ggaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACaa                            >  4:10901/1‑133 (MQ=255)                                                                                           ggaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACaa                            <  3:10901/133‑1 (MQ=255)                                                                                            gaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                           >  7:28882/1‑85 (MQ=255)                                                                                            gaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCAt                                                                           <  8:28882/85‑1 (MQ=255)                                                                                            gaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgtgt           <  1:124864/149‑1 (MQ=255)                                                                                                     acCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                          >  5:74153/1‑77 (MQ=255)                                                                                                     acCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                          <  6:74153/77‑1 (MQ=255)                                                                                                     acCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTg  >  1:23122/1‑149 (MQ=255)                                                                                                                            |                                                                                                                               TCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTG  >  NC_000913/4175023‑4175272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑