Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,523 C→A L96M (CTG→ATG)  yjbQ → thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,5230CA100.0% 92.2 / NA 27L96M (CTG→ATG) yjbQthiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/13);  total (14/13)

GACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAC  >  NC_000913/4270384‑4270662
                                                                                                                                           |                                                                                                                                           
gACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCCTTTTTTCCTCAGAACCGTTCCCGAAAACGCAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATAAAATCCTCAATGATGGGaaaa                                                                                                                                     >  7:45797/1‑146 (MQ=255)
        aTGAGAACTTCGATCCAAACTTACGCCACTTCATGGAGCGTTTTTTCCTCCGCACAGGTCCCGACAACGGAAATTATTAGCATGACTATGAGGGAGAATACGATATGCCTTCTCATATCAAATACTCAATGATGGTAACATAGCTTGTa                                                                                                                            <  8:45797/149‑1 (MQ=255)
               cTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCgg                                                                                                                     <  7:7859/149‑1 (MQ=255)
                tGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCg                                                                                                                      <  8:114907/147‑1 (MQ=255)
                tGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCg                                                                                                                      >  7:114907/1‑147 (MQ=255)
                                 ccACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAtt                                                                                                    >  8:48807/1‑148 (MQ=255)
                                  cACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAtt                                                                                                    >  7:129211/1‑147 (MQ=255)
                                           gAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCa                                                                                          <  1:162868/148‑1 (MQ=255)
                                           gAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCa                                                                                          >  2:162868/1‑148 (MQ=255)
                                                        tccGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGt                                                                                                                    <  8:2580/109‑1 (MQ=255)
                                                        tccGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGt                                                                                                                    >  7:2580/1‑109 (MQ=255)
                                                           gCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTgg                                                                         <  8:129211/149‑1 (MQ=255)
                                                                gTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                    <  7:48807/149‑1 (MQ=255)
                                                                             gAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATc                                                       >  8:104360/1‑149 (MQ=255)
                                                                                       gCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCa                                                                                                  <  1:170215/96‑1 (MQ=255)
                                                                                       gCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCa                                                                                                  >  2:170215/1‑96 (MQ=255)
                                                                                                tGAGGGCGCATACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAGTCAGACCGGCACCTGGCAAGGCAGTTGGCTGGGGGCACATCGCCTCCACGGCGGATCGCGTCg                                      >  6:101128/1‑147 (MQ=255)
                                                                                                   gggAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGca                                    >  4:24926/1‑146 (MQ=255)
                                                                                                   gggAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGca                                    <  3:24926/146‑1 (MQ=255)
                                                                                                    ggAGCAGACGATATGCCTTCTCATATCAGATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTgg                                                                                     >  6:4342/1‑96 (MQ=255)
                                                                                                    ggAGCAGACGATATGCCTTCTCATATCACATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTgg                                                                                     <  5:4342/96‑1 (MQ=255)
                                                                                                         aGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                           <  7:104360/149‑1 (MQ=255)
                                                                                                               tatGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCgg                                                                                                                     <  6:67316/53‑1 (MQ=255)
                                                                                                               tatGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCgg                                                                                                                     >  5:67316/1‑53 (MQ=255)
                                                                                                                            atCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTaaa         >  3:107346/1‑148 (MQ=255)
                                                                                                                             tCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTaaaa        >  3:45793/1‑148 (MQ=255)
                                                                                                                                  tCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAc  <  5:101128/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGAC  >  NC_000913/4270384‑4270662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: