Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,598:1 +C 6.0% coding (413/1269 nt) umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,5981.C 6.0% 336.6 / 19.3 83coding (413/1269 nt)umuCtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base . (37/41);  new base C (2/3);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

GAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGG  >  NC_000913/1231450‑1231742
                                                                                                                                                     |                                                                                                                                                
gAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTggg                                                                                                                                                   >  7:219964/1‑149 (MQ=255)
gAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTggg                                                                                                                                                   <  7:279736/149‑1 (MQ=255)
   gagCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGAt                                                                                                                                               >  2:46389/1‑149 (MQ=255)
   gagCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGAt                                                                                                                                               <  1:46389/149‑1 (MQ=255)
       tATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGcc                                                                                                                                           >  7:214804/1‑149 (MQ=255)
        aTCGCCACGCGACGAGATTTACACTATTGATGAGCCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTCGTGTGGGGATCGcc                                                                                                                                           >  2:403380/1‑148 (MQ=255)
         tCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGcc                                                                                                                                           >  3:80409/1‑147 (MQ=255)
         gcGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTTCGATCTGCCAGGGGGGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACACCGTACCCATCTTACTGTTGGTGTGGGGATCGCCCa                                                                                                                                         <  8:80841/148‑1 (MQ=255)
            ccccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAc                                                                                                                                      >  5:136041/1‑149 (MQ=255)
             cccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGAc                                                                                                                                      >  6:53141/1‑148 (MQ=255)
              ccGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGAcc                                                                                                                                     >  5:76340/1‑148 (MQ=255)
                 cgTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                 <  3:171118/149‑3 (MQ=255)
                   tCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACg                                                                                                                               >  3:87088/1‑149 (MQ=255)
                      agaTTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                            <  7:95693/149‑1 (MQ=255)
                       gaTTTACAGTATTGATGAGGCTTTCTGCGATCTGACAGGTGTGCGTTATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTg                                                                                                                            <  6:365762/148‑1 (MQ=255)
                           tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                 >  8:109343/1‑137 (MQ=255)
                           tACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaaca                                                                                                                                 <  7:109343/139‑3 (MQ=255)
                            aCAGTATTGATGCGGCATTCTGCGATCTGACAGGGGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTaa                                                                                                                       <  1:273691/148‑1 (MQ=255)
                               gTATTGATGAGGCATTCTGCGAGCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCtt                                                                                                                   <  8:212420/149‑1 (MQ=255)
                                        aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           <  6:76340/148‑1 (MQ=255)
                                        aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           <  2:250418/148‑1 (MQ=255)
                                        aGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           <  8:182337/148‑1 (MQ=255)
                                         ggCATTCTGCGATCTGACATGTGTGCGTAATTGTCGCGATCTGACTGATTTTGTCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATg                                                                                                         <  8:1206/149‑1 (MQ=255)
                                         ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                <  4:55894/126‑4 (MQ=255)
                                         ggCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTCTGGGCGATCCCCACACCaacag                                                                                                                                >  3:55894/1‑123 (MQ=255)
                                          gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACATCGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                        <  2:346421/149‑1 (MQ=255)
                                          gCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGc                                                                                                        >  3:21940/1‑149 (MQ=255)
                                           cATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACTGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCg                                                                                                       <  1:253696/149‑1 (MQ=255)
                                             ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                            <  3:59393/142‑1 (MQ=255)
                                             ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATc                                                                                                            >  4:59393/1‑142 (MQ=255)
                                             ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           <  3:81772/143‑1 (MQ=255)
                                             ttCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           >  4:81772/1‑143 (MQ=255)
                                               cTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCa                                                                                                    >  2:271203/1‑148 (MQ=255)
                                                tGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaa                                                                                                   >  6:207271/1‑148 (MQ=255)
                                                    aTCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                              >  2:300577/1‑149 (MQ=255)
                                                         aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                              <  3:266965/144‑1 (MQ=255)
                                                         aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCaaaaaaa                                                                                              <  8:339729/144‑1 (MQ=255)
                                                         aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAt                                                                                             <  6:323158/145‑1 (MQ=255)
                                                         aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATg                                                                                            <  5:207271/146‑1 (MQ=255)
                                                         aCAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCa                                                                                         <  1:300577/149‑1 (MQ=255)
                                                          cAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAg                                                                                        <  1:403380/149‑1 (MQ=255)
                                                           aGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGc                                                                                       <  8:219964/149‑1 (MQ=255)
                                                                tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           <  5:344899/124‑1 (MQ=255)
                                                                tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCa                                                                                                           >  6:344899/1‑124 (MQ=255)
                                                                tgCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAg                                                                                  >  5:271007/1‑149 (MQ=255)
                                                                  cGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGAc                                                                                >  8:318620/1‑149 (MQ=255)
                                                                    tAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgg                                                                              >  8:353210/1‑149 (MQ=255)
                                                                      aTTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggt                                                                            <  8:214804/149‑1 (MQ=255)
                                                                       ttGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtg                                                                           <  2:189970/149‑1 (MQ=255)
                                                                          tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                         <  4:21940/148‑1 (MQ=255)
                                                                          tCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACgggtggg                                                                         <  7:44717/148‑1 (MQ=255)
                                                                            gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                   <  8:352234/88‑1 (MQ=255)
                                                                            gcgATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCaa                                                                                                                                   >  7:352234/1‑88 (MQ=255)
                                                                                  tgactgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCACACAAAAACGCAGGCTAAGCTACCCAATCATGCGGCAAAAAAATGGAAGCGGCATACGGCTGGGGATGTGGAt                                                                >  3:44575/1‑149 (MQ=255)
                                                                                    actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAttt                                                              <  4:80409/149‑1 (MQ=255)
                                                                                    actgaTTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGGTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGa                                                                 <  6:134577/146‑1 (MQ=255)
                                                                                             ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGTATCGCCCAGACCAAAACGCTGGCTAAGGTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATc                                                      <  5:53141/148‑1 (MQ=255)
                                                                                             ggCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCAAAGCTTGCCAATAATGCGGCAAAAAAATGGCATCGGCAGACGGGTGGGTTGGTGGATTTATCAAAtat                                                     >  6:280627/1‑147 (MQ=255)
                                                                                              gCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCt                                                     <  6:136041/148‑1 (MQ=255)
                                                                                               cAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTg                                                    >  2:403057/1‑148 (MQ=255)
                                                                                                agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                    <  1:75700/131‑1 (MQ=255)
                                                                                                agagAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGggtggt                                                                    >  2:75700/1‑131 (MQ=255)
                                                                                                 gagaAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGaa                                                 >  3:10828/1‑149 (MQ=255)
                                                                                                      aTTCGCGCAACGGTGCTACATGGTACGCATCTTAATGTTTGTTTGGGGATCGCACAGACCAAATCGCGTGCTATGATTGCCATTCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTTGTGGATTTATGATATCTGGAACGCCa                                            <  4:44575/149‑1 (MQ=255)
                                                                                                      aTTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGCCCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGc                                              >  8:336709/1‑147 (MQ=255)
                                                                                                            gcAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTa                                       >  2:50471/1‑148 (MQ=255)
                                                                                                               aCGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGAAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAAt                                    >  1:294980/1‑148 (MQ=255)
                                                                                                                 ggTGCTACCACGTATCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTaa                                 <  5:183375/149‑1 (MQ=255)
                                                                                                                  gTGCTACAACGAACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAAt                                <  3:9531/149‑1 (MQ=255)
                                                                                                                   tGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATg                               >  6:403056/1‑149 (MQ=255)
                                                                                                                                  aTCTTACAGTTGGTCTGGGGATATACCACACCAAACCTCTGGCTAAGCTTGAAAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGCGAGGTGTATTTATCAAATCTCTAACGCCAGCGTAAATTAATTTATGCTCACCCAGTg                >  2:279478/1‑149 (MQ=255)
                                                                                                                                   tcataatGTTGGTGTGGCGATCGCCCAGACCAAAACGCAGGCTAAGCTTGCCAATCATTCGGCAAAAAAATGGCAGCGGCAGACGGGTGTGGTGGTGGATTTATCAAATCTGTAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTgg               <  2:294980/143‑1 (MQ=255)
                                                                                                                                   tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                >  7:71572/1‑148 (MQ=255)
                                                                                                                                   tCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTg                >  7:54113/1‑148 (MQ=255)
                                                                                                                                       aCTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGa              <  7:353210/146‑1 (MQ=255)
                                                                                                                                        cTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGa           <  8:71572/148‑1 (MQ=255)
                                                                                                                                           ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc       >  7:64604/1‑149 (MQ=255)
                                                                                                                                           ttGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTc       <  7:318620/149‑1 (MQ=255)
                                                                                                                                               tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                               >  1:40264/1‑89 (MQ=255)
                                                                                                                                               tgtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGAtt                                                               <  2:40264/89‑1 (MQ=255)
                                                                                                                                                gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAACATGGCAGCGGCAGCCGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCGCCGTGGAGGCCGTCTgggg  >  4:145798/1‑149 (MQ=255)
                                                                                                                                                gtgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg  >  1:401229/1‑149 (MQ=255)
                                                                                                                                                 tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCAGTGGATGACGTCTgggg  >  8:98410/1‑148 (MQ=255)
                                                                                                                                                 tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTgggg  <  3:140273/148‑1 (MQ=255)
                                                                                                                                                 tgGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAAGGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTgggg  >  1:110934/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGGGATCGCCCAGACCAAAACGCTGGCTAAGCTTGCCAATCATGCGGCAAAAAAATGGCAGCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGG  >  NC_000913/1231450‑1231742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: