Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,270,523 C→A 100% L96M (CTG→ATG)  yjbQ → thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,5230CA98.2% 384.5 / ‑2.2 111L96M (CTG→ATG) yjbQthiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (61/48);  minor base T (1/1);  total (62/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 115 total aligned reads displayed.

CCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTC  >  NC_000913/4270377‑4270668
                                                                                                                                                  |                                                                                                                                                 
ccTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAatgatg                                                                                                                                                 <  3:379150/149‑1 (MQ=255)
ccTCTCTGACACTTAATGAGAACGGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAatgatg                                                                                                                                                 <  3:268089/149‑1 (MQ=255)
ccTCTCTAACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAatgatg                                                                                                                                                 >  5:160362/1‑149 (MQ=255)
 ctctctAACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATgg                                                                                                                                                <  6:160362/149‑1 (MQ=255)
  tctctGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATgg                                                                                                                                                >  5:173775/1‑148 (MQ=255)
    tctGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATggg                                                                                                                                               <  7:212694/147‑1 (MQ=255)
    tctGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGaa                                                                                                                                             >  6:42789/1‑149 (MQ=255)
      tGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACa                                                                                                                                           >  1:127767/1‑149 (MQ=255)
      tGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACa                                                                                                                                           >  3:314926/1‑149 (MQ=255)
        acacTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACa                                                                                                                                           <  8:46661/147‑1 (MQ=255)
          acTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGc                                                                                                                                       >  1:24201/1‑149 (MQ=255)
            ttAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCtt                                                                                                                                     >  2:263729/1‑149 (MQ=255)
              aaTGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCtt                                                                                                                                     <  2:127767/147‑1 (MQ=255)
                     aCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCg                                                                                                                            <  2:24201/149‑1 (MQ=255)
                       tGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGt                                                                                                                          >  8:361945/1‑149 (MQ=255)
                         cGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATTTGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGt                                                                                                                          <  2:360843/147‑1 (MQ=255)
                         cGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGc                                                                                                                        <  7:78175/149‑1 (MQ=255)
                           aTCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCa                                                                                                                       >  5:125991/1‑148 (MQ=255)
                                aCCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAgg                                                                                                                 <  7:235711/149‑1 (MQ=255)
                                  cGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGc                                                                                                               <  4:363883/149‑1 (MQ=255)
                                       gCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAt                                                                                                           >  8:228018/1‑148 (MQ=255)
                                       gCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGAGCAtt                                                                                                          >  6:106815/1‑149 (MQ=255)
                                         cACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAtt                                                                                                          >  4:67686/1‑147 (MQ=255)
                                         cACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCa                                                                                                        <  3:67686/149‑1 (MQ=255)
                                            gACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTTTGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATaaa                                                                                                                   >  7:300540/1‑135 (MQ=255)
                                            gACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATaaa                                                                                                                   <  8:300540/135‑1 (MQ=255)
                                            gACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGAc                                                                                                     <  1:263729/149‑1 (MQ=255)
                                               aTGGAGCGTTTTTTCCTCCGCACCGTTCCAGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGAACgg                                                                                                  >  1:198324/1‑149 (MQ=255)
                                                  gAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGACCATGACTATGAGGTAGCAGACGATATGCCTTCTCATATCAAAACCACAATGATGGTAACCTCGCATGAATTGCACGTCAATAAAGGTCTTATTCAGACCGCCa                                                                                                >  4:224289/1‑148 (MQ=255)
                                                   aGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCAcc                                                                                              >  8:366002/1‑149 (MQ=255)
                                                    gCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCt                                                                                             <  4:196001/149‑1 (MQ=255)
                                                     cGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGTTGGGAACATCGCTTGTATTGCCGGTGCATaaa                                                                                                                   >  6:28616/1‑126 (MQ=255)
                                                     cGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGTTGGGAACATCGCTTGTATTGCCGGTGCATaaa                                                                                                                   <  5:28616/126‑1 (MQ=255)
                                                       ttttttCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTgg                                                                                           >  1:143345/1‑148 (MQ=255)
                                                          tttCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCaa                                                                                        <  7:366002/148‑1 (MQ=255)
                                                              ctccGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCAt                                                                                   >  7:129016/1‑149 (MQ=255)
                                                               tccGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCAtt                                                                                  >  7:418144/1‑149 (MQ=255)
                                                                   cACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGc                                                                              <  6:173775/149‑1 (MQ=255)
                                                                    aCCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGc                                                                                                                              <  8:221826/100‑1 (MQ=255)
                                                                    aCCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCt                                                                             <  7:163020/149‑1 (MQ=255)
                                                                      cGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTgg                                                                           <  7:228018/149‑1 (MQ=255)
                                                                       gTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                          <  7:109704/149‑1 (MQ=255)
                                                                       gTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                          <  1:140443/149‑1 (MQ=255)
                                                                       gTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                          <  3:104026/149‑1 (MQ=255)
                                                                            cGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGaa                                                                      >  5:129990/1‑148 (MQ=255)
                                                                            cGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAAc                                                                     <  5:42789/149‑1 (MQ=255)
                                                                             gACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACa                                                                    <  4:141510/149‑1 (MQ=255)
                                                                                  cGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa                                                               >  8:231909/1‑149 (MQ=255)
                                                                                  cGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCa                                                               >  2:60570/1‑149 (MQ=255)
                                                                                      aaTTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCa                                                           <  6:125991/149‑1 (MQ=255)
                                                                                       aTTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCAc                                                          >  5:221174/1‑149 (MQ=255)
                                                                                           tGAGCATGACTATGATGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAAAATCGCTTGTATTGCCGGTGCATAAAGGGCGGAGTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCAcggc                                                       <  3:224289/148‑1 (MQ=255)
                                                                                           tGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCAcggcg                                                      >  4:285622/1‑149 (MQ=255)
                                                                                            gAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCAcggcg                                                      >  3:351877/1‑148 (MQ=255)
                                                                                             aGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGa                                                    <  5:186224/149‑1 (MQ=255)
                                                                                             aGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGa                                                    <  2:143345/149‑1 (MQ=255)
                                                                                             aGCAAGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCAATTGGATAGGGGAACATCGCATCCAcggcgg                                                     >  5:348398/1‑148 (MQ=255)
                                                                                              gCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGAt                                                   <  3:285622/149‑1 (MQ=255)
                                                                                                aTGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcg                                                 >  7:37982/1‑149 (MQ=255)
                                                                                                 tGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGCCCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATcgc                                                >  8:59085/1‑149 (MQ=255)
                                                                                                 tGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                          >  3:274594/1‑123 (MQ=255)
                                                                                                 tGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTggg                                                                          <  4:274594/123‑1 (MQ=255)
                                                                                                 tGACTATGAGGGAGCAGACGATATGCCTTCTCATATAAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTAATCAGACCGGCACCTGGAAATGCATTTGGCTGGGGTAACATCGCATCCACGGCGGATcgc                                                >  6:362986/1‑149 (MQ=255)
                                                                                                     tATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCg                                            >  2:80034/1‑149 (MQ=255)
                                                                                                     tATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCg                                            >  3:355787/1‑149 (MQ=255)
                                                                                                     tATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGAGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCg                                            >  6:113030/1‑149 (MQ=255)
                                                                                                        gAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGcat                                         >  3:311379/1‑149 (MQ=255)
                                                                                                          gggAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGcatca                                       >  1:297884/1‑149 (MQ=255)
                                                                                                           ggAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAAAATCGCTTGTATTGCCGGTGCATAAAGGGCGTTTTCAGACCGGCTCCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGcatcat                                      <  5:362986/149‑1 (MQ=255)
                                                                                                                aGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                 >  7:151324/1‑149 (MQ=255)
                                                                                                                aGACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                 >  8:251850/1‑149 (MQ=255)
                                                                                                                 gACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGTCTGGGGGAACATCGCATCAACGGCGGATCGCGTCGCATCATCGCGa                                 >  5:320818/1‑148 (MQ=255)
                                                                                                                 gACGATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGa                                 >  1:239454/1‑148 (MQ=255)
                                                                                                                    gATATGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGAGCGCGTCGCATCATCGCGACACt                             <  6:203759/149‑1 (MQ=255)
                                                                                                                      tatGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCtt                                                                                                                                     <  7:223539/43‑1 (MQ=255)
                                                                                                                      tatGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCtt                                                                                                                                     >  8:223539/1‑43 (MQ=255)
                                                                                                                      tatGCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTAc                           <  6:129990/149‑1 (MQ=255)
                                                                                                                         gCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCatcatc                                     >  8:414194/1‑136 (MQ=255)
                                                                                                                         gCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCatcatc                                     <  7:414194/136‑1 (MQ=255)
                                                                                                                         gCCTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAg                        >  2:231907/1‑149 (MQ=255)
                                                                                                                          ccTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAgg                       <  7:33544/149‑1 (MQ=255)
                                                                                                                           cTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAggg                      >  8:58023/1‑149 (MQ=255)
                                                                                                                           cTTCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAggg                      <  2:297884/149‑1 (MQ=255)
                                                                                                                            ttCTCATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAggg                      >  5:122398/1‑148 (MQ=255)
                                                                                                                              ctcATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAgggg                     <  6:221174/147‑1 (MQ=255)
                                                                                                                               tcATATCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAg                   >  6:283007/1‑148 (MQ=255)
                                                                                                                                 atatCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAAGATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAg                   <  3:161534/146‑1 (MQ=255)
                                                                                                                                 atatCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTa                 >  4:109452/1‑148 (MQ=255)
                                                                                                                                 atatCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTa                 >  6:225061/1‑148 (MQ=255)
                                                                                                                                    tCAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGt                                              <  1:193269/116‑1 (MQ=255)
                                                                                                                                     cAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTaaaaaa            >  8:114386/1‑149 (MQ=255)
                                                                                                                                     cAAATCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGAGACACTACAAGGGGAGTaaaaaa            >  1:19047/1‑149 (MQ=255)
                                                                                                                                      aaaTCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTgg                                                                           <  2:351128/85‑1 (MQ=255)
                                                                                                                                      aaaTCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTgg                                                                           >  1:351128/1‑85 (MQ=255)
                                                                                                                                      aaaTCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTaaaaaa            <  8:151324/148‑1 (MQ=255)
                                                                                                                                      aaaTCCTCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTaaaaaa            <  7:351240/148‑1 (MQ=255)
                                                                                                                                            tCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCAt     >  7:166010/1‑149 (MQ=255)
                                                                                                                                            tCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCAt     >  6:69883/1‑149 (MQ=255)
                                                                                                                                            tCAATGATGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCAt     >  1:212976/1‑149 (MQ=255)
                                                                                                                                               atgatgGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTc  >  7:79807/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTC  >  NC_000913/4270377‑4270668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: