Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,216,188 C→T 5.2% D102N (GAT→AAT)  yqjH ← putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,216,1880CT 5.2% 251.7 / 2.4 77D102N (GAT→AAT) yqjHputative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base T (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGT  >  NC_000913/3216041‑3216334
                                                                                                                                                   |                                                                                                                                                  
ttCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatc                                                                                                                                                    >  8:361136/1‑148 (MQ=255)
ttCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatc                                                                                                                                                    >  8:345204/1‑148 (MQ=255)
   cGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatcaatc                                                                                                                                                <  7:345204/149‑1 (MQ=255)
   cGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatcaatc                                                                                                                                                >  8:178534/1‑149 (MQ=255)
   cGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAaatcaatc                                                                                                                                                <  8:240255/149‑1 (MQ=255)
        tCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAg                                                                                                                                           >  1:75937/1‑149 (MQ=255)
          aTCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATTAATCGCCAGt                                                                                                                                          >  4:316739/1‑148 (MQ=255)
          aTCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGtt                                                                                                                                         >  6:213217/1‑149 (MQ=255)
           tCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATTAATCGCCa                                                                                                                                            <  4:43/145‑1 (MQ=255)
           tCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATTAATCGCCa                                                                                                                                            >  3:43/1‑145 (MQ=255)
             gCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCa                                                                                                                                       <  4:355961/148‑1 (MQ=255)
             gCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCAt                                                                                                                                      <  5:157608/149‑1 (MQ=255)
                  cATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGt                                                                                                                                 <  7:178534/149‑1 (MQ=255)
                     aCAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGt                                                                                                                              >  3:306053/1‑149 (MQ=255)
                      cAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATTAATCGCCAGTTCATGGCGTAGtt                                                                                                                             <  3:316739/149‑1 (MQ=255)
                      cAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGtt                                                                                                                             <  7:361136/149‑1 (MQ=255)
                         cTGATACGCGTAATCTTCCGGCACCACCAGCGAACCTCGCGGACCTGCCACCGTAATTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGGTCg                                                                                                                           >  7:87536/1‑148 (MQ=255)
                            aTACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTc                                                                                                                         <  4:89241/147‑1 (MQ=255)
                            aTACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCa                                                                                                                        <  8:155976/148‑1 (MQ=255)
                             tACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATa                                                                                                                      <  6:173284/149‑1 (MQ=255)
                             tACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATa                                                                                                                      >  4:363865/1‑149 (MQ=255)
                                    aaTCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAgcggc                                                                                                               >  1:308582/1‑149 (MQ=255)
                                        ttCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGta                                                                                                            >  3:261727/1‑148 (MQ=255)
                                        ttCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCCGCGta                                                                                                            >  1:198572/1‑148 (MQ=255)
                                          ccGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGta                                                                                                            <  1:174420/146‑1 (MQ=255)
                                             gCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCa                                                                                                      <  6:149138/149‑1 (MQ=255)
                                                ccaccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAcgc                                                                                                   >  4:330735/1‑149 (MQ=255)
                                                 caccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAgc                                                                                                                  <  8:237119/133‑1 (MQ=255)
                                                 caccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAgc                                                                                                                  >  7:237119/1‑133 (MQ=255)
                                                 caccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAcgcg                                                                                                  >  1:64960/1‑149 (MQ=255)
                                                 caccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAcgc                                                                                                   >  6:71691/1‑148 (MQ=255)
                                                 caccaGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCAcgc                                                                                                   >  6:99718/1‑148 (MQ=255)
                                                    caGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACg                                                                                               >  3:149100/1‑149 (MQ=255)
                                                     aGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACgg                                                                                              >  8:222311/1‑149 (MQ=255)
                                                      gCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGt                                                                                             >  5:47127/1‑149 (MQ=255)
                                                       cGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTa                                                                                                                                >  3:149676/1‑113 (MQ=255)
                                                       cGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTa                                                                                                                                <  4:149676/113‑1 (MQ=255)
                                                       cGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTc                                                                                                                         >  4:47794/1‑120 (MQ=255)
                                                       cGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTc                                                                                                                         <  3:47794/120‑1 (MQ=255)
                                                          aCCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGc                                                                                         <  2:75937/149‑1 (MQ=255)
                                                             gcgcgGACCTGCCACCGTAAGTTTATCGCCCGGTTTCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTg                                                                                      <  7:221921/149‑1 (MQ=255)
                                                             gcgcgGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTg                                                                                      <  6:255918/149‑1 (MQ=255)
                                                             gcgcgGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTg                                                                                      <  7:222311/149‑1 (MQ=255)
                                                             gcgcgGACCTGCCACCGTAAGAGTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCGGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTg                                                                                      <  1:291533/149‑1 (MQ=255)
                                                                    ccTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTc                                                                               <  3:363865/149‑1 (MQ=255)
                                                                             gTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGAc                                                                      >  7:78718/1‑149 (MQ=255)
                                                                                    tctCGCCCGGTTGCGCCGGAATCGCCCAGCCGCTGGCGCCCCCACCGGCGTGCCTAAAGAACTCAATCGCCCGTTCATGGCGTCGTTCTGCATCCAGCGGCGTATCGTCACGCGACGGTGTGCGTGGTCCTTCCGGCCAGACGATGcc                                                                <  8:139654/146‑1 (MQ=255)
                                                                                     aTCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGccc                                                               <  4:306053/148‑1 (MQ=255)
                                                                                          ccGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTcc                                                         <  7:39757/149‑1 (MQ=255)
                                                                                          ccGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTcc                                                         <  8:242217/149‑1 (MQ=255)
                                                                                            ggTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGt                                                       <  4:107020/149‑1 (MQ=255)
                                                                                                   ccTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGtt                                                <  8:78718/149‑1 (MQ=255)
                                                                                                     tGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGtata                                                                                                          >  1:278796/1‑89 (MQ=255)
                                                                                                     tGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGtata                                                                                                          <  2:278796/89‑1 (MQ=255)
                                                                                                      gCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTc                                                                                                                         <  3:173894/73‑1 (MQ=255)
                                                                                                      gCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTc                                                                                                                         >  4:173894/1‑73 (MQ=255)
                                                                                                             ccAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGcaca                                       >  5:370292/1‑148 (MQ=255)
                                                                                                             ccAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACaa                                      <  2:64960/149‑1 (MQ=255)
                                                                                                               aGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAg                                    >  6:39396/1‑149 (MQ=255)
                                                                                                                  cGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACgg                                                                                              >  1:146708/1‑88 (MQ=255)
                                                                                                                  cGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACgg                                                                                              <  2:146708/88‑1 (MQ=255)
                                                                                                                    cTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGc                               >  2:51009/1‑149 (MQ=255)
                                                                                                                     tGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCg                              >  6:139745/1‑149 (MQ=255)
                                                                                                                       gCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACTCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGt                             <  5:213217/148‑1 (MQ=255)
                                                                                                                        cGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCa                           <  3:330735/149‑1 (MQ=255)
                                                                                                                          aCCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAgg                         >  2:140820/1‑149 (MQ=255)
                                                                                                                            cccACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGtt                       >  5:124114/1‑149 (MQ=255)
                                                                                                                             cccccGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCTTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGtt                       >  7:259950/4‑148 (MQ=255)
                                                                                                                                     gtGAAAAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCCCGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAaaga              <  8:378454/149‑1 (MQ=255)
                                                                                                                                      tGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAaagaa             <  6:47127/149‑1 (MQ=255)
                                                                                                                                      tGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAaagaa             <  1:51009/149‑1 (MQ=255)
                                                                                                                                       gAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAaag               >  4:231538/1‑146 (MQ=255)
                                                                                                                                        aaTAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAaagaag            <  3:231538/148‑1 (MQ=255)
                                                                                                                                         aTAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAagag          >  7:410828/1‑149 (MQ=255)
                                                                                                                                          tAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTc                                                          <  4:14308/100‑1 (MQ=255)
                                                                                                                                          tAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGt         >  1:249041/1‑149 (MQ=255)
                                                                                                                                          tAAAGAAATCAATCGCAAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTc                                                          >  3:14308/1‑100 (MQ=255)
                                                                                                                                             aGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAagaaga           >  5:237666/1‑144 (MQ=255)
                                                                                                                                             aGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAagaaga           <  6:237666/144‑1 (MQ=255)
                                                                                                                                             aGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTg      >  8:212139/1‑149 (MQ=255)
                                                                                                                                             aGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTg      >  6:156241/1‑149 (MQ=255)
                                                                                                                                               aaaTCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCt    >  1:128382/1‑149 (MQ=255)
                                                                                                                                                 atcaatcGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCtgt  <  2:308582/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCGGATTCATCGCAGACATACAGCTGATACGCGTAATCTTCCGGCACCACCAGCGAACCGCGCGGACCTGCCACCGTAAGTTTATCGCCCGGTTGCGCCTGCATCGCCCAGCCGCTGGCGACCCCACCGTCGTGAATAAAGAAATCAATCGCCAGTTCATGGCGTAGTTCGTCATACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGT  >  NC_000913/3216041‑3216334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: