Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,182,938 A→C 13.6% E565A (GAA→GCA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9380AC13.6% 296.4 / 34.0 121E565A (GAA→GCA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (55/49);  new base C (9/8);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACT  >  NC_000913/4182790‑4183084
                                                                                                                                                    |                                                                                                                                                  
gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                                                                                                                                                    <  2:199649/149‑1 (MQ=255)
    aGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt                                                                                                                                                <  4:129530/149‑1 (MQ=255)
     gAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTc                                                                                                                                               >  3:73545/1‑149 (MQ=255)
      aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTcc                                                                                                                                              >  8:34053/1‑149 (MQ=255)
      aacaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGCCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAACCCCCTGAAGGTcc                                                                                                                                              >  5:256103/1‑149 (MQ=255)
         aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCACTCGAAACCCCTGAAGGTCCGaa                                                                                                                                           <  3:246699/149‑1 (MQ=255)
              gCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                                      <  4:9450/149‑1 (MQ=255)
               cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCgg                                                                                                                                     <  4:73545/149‑1 (MQ=255)
                  tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                                                                    <  3:374721/147‑1 (MQ=255)
                    tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa                                                                                                                                >  8:419262/1‑149 (MQ=255)
                    tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCAACGCGGTCTGACACGTGAACGTGCAGGCTTCGAAGTTCGAGAAGTACAACCTACTCCCTACGGTCTCGTATGTCCAATCGAAACCCCTGAAGGACCGATCATCGCTCTg                                                                                                                                 >  4:345304/1‑148 (MQ=255)
                      agaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGAt                                                                                                                               <  4:388307/148‑1 (MQ=255)
                      agaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa                                                                                                                                <  7:205845/147‑1 (MQ=255)
                       gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATc                                                                                                                              >  8:348500/1‑148 (MQ=255)
                         ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAAc                                                                                                                           <  7:34053/149‑1 (MQ=255)
                          tACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                                          >  1:215918/1‑149 (MQ=255)
                           aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAct                                                                                                                          <  8:175652/148‑1 (MQ=255)
                              cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctct                                                                                                                      <  4:220318/149‑1 (MQ=255)
                               acaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGGATGTCCAATCGAAAACCCTGAAGTTCCGAACATCGGTCTGATCAACTCTCTg                                                                                                                     >  5:118542/1‑149 (MQ=255)
                                caAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                                                                      <  8:149278/131‑1 (MQ=255)
                                 aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                                                                   >  4:354442/1‑149 (MQ=255)
                                 aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                                                                   >  2:325787/1‑149 (MQ=255)
                                    cgtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCACCTCTCTGTCCgt                                                                                                                >  6:171028/1‑149 (MQ=255)
                                        gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                                            <  6:193154/149‑1 (MQ=255)
                                          aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCCGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGCAGGTCCGAACATCGGTCTGATCAGCTCTCTGTCCGTGTACGc                                                                                                          >  1:171198/1‑149 (MQ=255)
                                           tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                                         >  6:37793/1‑149 (MQ=255)
                                             tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctct                                                                                                                      <  4:288888/134‑1 (MQ=255)
                                               cGCACTGGGCCCTGGCGGTCTGACACGTGAACGTGCTGGCTTCGAAGTTCGAGACGTACACCCGACTCACTCCGGTCGCGTATGTCCACTCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                                         <  8:134261/145‑1 (MQ=255)
                                               cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca                                                                                                       <  8:418645/147‑1 (MQ=255)
                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                                                                >  8:263710/1‑149 (MQ=255)
                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                                                                              >  7:191783/1‑135 (MQ=255)
                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                                                                              <  8:191783/135‑1 (MQ=255)
                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                                         <  1:365576/140‑1 (MQ=255)
                                                    tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                                         >  2:365576/1‑140 (MQ=255)
                                                      ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                                                                <  1:21017/147‑1 (MQ=255)
                                                      ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTGCGAAGTGCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAc                                                                                                <  6:389217/147‑1 (MQ=255)
                                                      ggCCCAGGCGGTCTGACACGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACg                                                                                               >  2:311569/1‑148 (MQ=255)
                                                        cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCACCTCTCTGTCCGTGTACGCACAGACTAACGaa                                                                                             >  6:164370/1‑148 (MQ=255)
                                                        cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGaa                                                                                             >  7:350660/1‑148 (MQ=255)
                                                        cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAAt                                                                                            <  7:348500/149‑1 (MQ=255)
                                                          ccggCGGTCTGACCCGGGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                                                                          <  3:121793/147‑1 (MQ=255)
                                                           aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACTTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg                                                                                         <  1:131539/149‑1 (MQ=255)
                                                           aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTCCACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGCTCAACTCTATGTCCGTGTACGCACAGCCTAACGAATACg                                                                                         >  7:291582/1‑149 (MQ=255)
                                                           aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCCCTACGGTCGCGTATGTCCACTCGAAACCCCGGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGGGGACGCACAGACTAACGAATACg                                                                                         >  3:25778/1‑149 (MQ=255)
                                                               ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt                                                                                     >  7:40430/1‑149 (MQ=255)
                                                                   tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATAAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                                 >  8:292305/1‑149 (MQ=255)
                                                                     aCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga                                                                               <  1:54450/149‑1 (MQ=255)
                                                                       ccGTGAACGTGCTGGCTTCGTAGTTCGAGACGTACTCCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGCAGGTCCGATCATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga                                                                             <  5:171028/149‑1 (MQ=255)
                                                                       ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAACCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga                                                                             >  4:294352/1‑149 (MQ=255)
                                                                       ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                                                                              <  8:236166/116‑1 (MQ=255)
                                                                       ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                                                                              >  7:236166/1‑116 (MQ=255)
                                                                          tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc                                                                          >  2:245281/1‑149 (MQ=255)
                                                                              cGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa                                                                      <  7:419262/149‑1 (MQ=255)
                                                                               gTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt                                                                     >  4:337689/1‑149 (MQ=255)
                                                                                tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                                    >  5:341906/1‑149 (MQ=255)
                                                                                 gCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCg                                                                   >  3:165582/1‑149 (MQ=255)
                                                                                    ggCTTCGAAGTTCGAGACGTACACCCGCCTCACTACGGTCGCGTATGTCCAATAGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa                                                                 >  2:71919/1‑148 (MQ=255)
                                                                                     gCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACCTCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa                                                                 >  6:33040/1‑147 (MQ=255)
                                                                                       ttCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc                                                                                                                         <  2:294633/89‑1 (MQ=255)
                                                                                       ttCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc                                                                                                                         >  1:294633/1‑89 (MQ=255)
                                                                                          gAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa                                                           <  2:280569/148‑1 (MQ=255)
                                                                                          gAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc                                                          >  1:84798/1‑149 (MQ=255)
                                                                                                     cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttg                                               <  2:84798/149‑1 (MQ=255)
                                                                                                      gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttg                                               >  6:149920/1‑148 (MQ=255)
                                                                                                       tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttgt                                              >  4:107769/1‑148 (MQ=255)
                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                                 <  5:396074/112‑1 (MQ=255)
                                                                                                        acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                                 >  6:396074/1‑112 (MQ=255)
                                                                                                          agccGACTCACTAGGGTAGCGTATGTGCAATCGAAACCCCTGAAGGTCAGAAAATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAATAAAGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTTTTGTAACt                                          <  4:52241/147‑1 (MQ=255)
                                                                                                            ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                                        >  7:368794/1‑149 (MQ=255)
                                                                                                            ccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                                        <  4:225918/149‑1 (MQ=255)
                                                                                                             cGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAc                                       >  8:275008/1‑149 (MQ=255)
                                                                                                              gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAc                                       >  5:85411/1‑148 (MQ=255)
                                                                                                                   aCTACGGTCTCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTATGATCAACTCTCTCTCCGTTTAAGCACAGACTTACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAAtt                                 <  3:345304/149‑1 (MQ=255)
                                                                                                                   aCTACGGTCGCGTATGTCCAATCGAACCCCCTCAAGGTCCGAACATCGGTCTGATCAACACTCTGTCCGTGTACGCACAGACTAACCAATACGGCTTCCTTGAGCCTCCGTATCGTAAAGTGACCGACGGTGTTCTAACTGACGAAAtt                                 >  2:135513/1‑149 (MQ=255)
                                                                                                                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCa                               >  2:141528/1‑149 (MQ=255)
                                                                                                                        ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTa                            <  6:341906/149‑1 (MQ=255)
                                                                                                                          tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc                          <  6:256103/149‑1 (MQ=255)
                                                                                                                          tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc                          >  5:154001/1‑149 (MQ=255)
                                                                                                                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGgtg                                                  <  2:382432/123‑1 (MQ=255)
                                                                                                                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGgtg                                                  >  1:382432/1‑123 (MQ=255)
                                                                                                                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa                                   <  7:78439/138‑1 (MQ=255)
                                                                                                                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGaaa                                   >  8:78439/1‑138 (MQ=255)
                                                                                                                                aTGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                                        >  3:16811/1‑129 (MQ=255)
                                                                                                                                aTGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGa                                        <  4:16811/129‑1 (MQ=255)
                                                                                                                                 tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGc                   >  5:59493/1‑149 (MQ=255)
                                                                                                                                   tCCAATCGAAACCGCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTa                 <  8:291582/149‑1 (MQ=255)
                                                                                                                                    ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                                 >  6:212323/1‑84 (MQ=255)
                                                                                                                                    ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                                 <  5:212323/84‑1 (MQ=255)
                                                                                                                                     cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATc               >  5:229248/1‑149 (MQ=255)
                                                                                                                                      aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATc               >  8:224252/1‑148 (MQ=255)
                                                                                                                                        tCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCg              >  8:261926/1‑147 (MQ=255)
                                                                                                                                          gAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCgaaga          <  1:325787/149‑1 (MQ=255)
                                                                                                                                          gAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCgaaga          <  3:337689/149‑1 (MQ=255)
                                                                                                                                           aaaCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCgaagaa         <  6:154001/149‑1 (MQ=255)
                                                                                                                                            aaCCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGaagaag        <  7:263710/149‑1 (MQ=255)
                                                                                                                                            aaCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGaagaag        <  3:354442/149‑1 (MQ=255)
                                                                                                                                               cccTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGc      >  4:134995/1‑148 (MQ=255)
                                                                                                                                               cccTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGc      >  8:370981/1‑148 (MQ=255)
                                                                                                                                               cccTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCa     <  6:229248/149‑1 (MQ=255)
                                                                                                                                                   gAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCaa    >  7:286442/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACT  >  NC_000913/4182790‑4183084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: