Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,151	(A)6→5	intergenic (‑125/‑237)	coaA ← / → thrU	pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,146	0	A	.	100.0%	122.0 / NA	28	intergenic (‑120/‑242)	coaA/thrU	pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (15/13);  total (15/13)

TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCG  >  NC_000913/4174999‑4175283                                                                                                                                                    |                                                                                                                                          tCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCGCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGaa                                                                                                                                          <  5:73185/149‑1 (MQ=255)                 ttttATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGaa                                                                                                                         >  6:97759/1‑149 (MQ=255)                      taCTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTAt                                                                                                                    <  6:39269/149‑1 (MQ=255)                         tCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGt                                                                                                                 >  7:1158/1‑149 (MQ=255)                               gCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGaa                                                                                                             >  3:40164/1‑147 (MQ=255)                                        tGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                      <  1:50954/145‑1 (MQ=255)                                        tGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAAt                                                                                                      >  2:50954/1‑145 (MQ=255)                                                   gCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTcc                                                                                        <  5:97759/148‑1 (MQ=255)                                                    ctctATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                >  5:104384/1‑139 (MQ=255)                                                    ctctATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACa                                                                                                <  6:104384/139‑1 (MQ=255)                                                           ggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                     <  1:97733/143‑1 (MQ=255)                                                           ggCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATc                                                                                     >  2:97733/1‑143 (MQ=255)                                                                           ccATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTa                                                               <  4:40164/149‑1 (MQ=255)                                                                                 cgcgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGa                                                         <  8:1158/149‑1 (MQ=255)                                                                                  gcgTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAg                                                        >  8:114657/1‑149 (MQ=255)                                                                                           ttCTGCTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                    <  5:124089/128‑1 (MQ=255)                                                                                           ttCTGATGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATg                                                                    >  6:124089/1‑128 (MQ=255)                                                                                                ttGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGc                                           >  4:130923/1‑148 (MQ=255)                                                                                                     tGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAat                                      >  1:141116/1‑148 (MQ=255)                                                                                                             tGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTa                                                               <  6:67344/115‑1 (MQ=255)                                                                                                                    gaggGTAACACCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAgtgt                      <  2:141116/149‑1 (MQ=255)                                                                                                                            cacCAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAACAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCt              <  7:114657/149‑1 (MQ=255)                                                                                                                              ccAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatga                                    >  5:130966/1‑125 (MQ=255)                                                                                                                              ccAGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAatga                                    <  6:130966/125‑1 (MQ=255)                                                                                                                                aGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGccc          >  5:121784/1‑149 (MQ=255)                                                                                                                                aGATGGGGAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGccc          >  1:18832/1‑149 (MQ=255)                                                                                                                                     gggAGGAATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGt     >  7:40574/1‑149 (MQ=255)                                                                                                                                      ggaggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGtt    >  3:14291/1‑149 (MQ=255)                                                                                                                                        aggaATAATAAG‑AAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCg  <  3:130923/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                          TCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATGGGTTAATGGGCAGGAGGGTAACACCAGATGGGGAGGAATAATAAGAAAAAATCTCTGCCAGGAAGCTATCGTTGAAAAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCG  >  NC_000913/4174999‑4175283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑