Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,335,761 IS3 (–) +3 bp :: +TCA 100% intergenic (+303/‑68) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 315229 =NA (NA)19 (0.170)
+TCA
19/244 NT 100% noncoding (1/1255 nt) IS3 repeat region
?NC_000913 1335761 = 0 (0.000)intergenic (+303/‑70) yciX/acnA uncharacterized protein/aconitate hydratase 1; aconitase A
* ? NC_000913 = 13357630 (0.000)27 (0.240) 23/246 NT 100% intergenic (+305/‑68) yciX/acnA uncharacterized protein/aconitate hydratase 1; aconitase A
?NC_000913 = 2171428 NA (NA)noncoding (1258/1258 nt) IS3 repeat region

GTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCA  >  NC_000913/1335613‑1335892
                                                                                                                                                    |                                                                                                                                   
tcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcag                                                                                                                                     <  8:28448‑M2/1‑1 (MQ=255)
 caggaagctgcagggcttcactgcgaaattgaggtgactgctgtttactgggttttttactgggtgatactgtttttgtcatgtgagtgacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcag                                                                                                                                     <  2:233517‑M2/1‑1 (MQ=255)
 cagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcag                                                                                                                                     <  1:376929‑M2/1‑1 (MQ=255)
  agcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGt                                                                                                                                    <  7:302791‑M2/2‑1 (MQ=255)
        tGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgat                                                                                                                               <  3:436958‑M1/147‑5 (MQ=255)
        cttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttattcacttagccgcgtgtccactattgctgggtaagatcaGt                                                                                                                                    <  1:272802‑M2/2‑1 (MQ=255)
        cttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttattcacttagccgcgtgtccactattgctgggtaagatcaGt                                                                                                                                    >  2:272802‑M2/141‑142 (MQ=255)
        cttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTgtgt                                                                                                                              >  6:83956‑M2/141‑148 (MQ=255)
                   ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaa                                                                                                                  >  1:237903‑M1/1‑132 (MQ=255)
                   ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaa                                                                                                                  >  7:58837‑M1/1‑132 (MQ=255)
                   ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaa                                                                                                                  >  1:282155‑M1/1‑132 (MQ=255)
                   cactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAatat                                                                                                                  >  1:305863‑M2/130‑149 (MQ=255)
                     ctgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATAtt                                                                                                                 >  8:207231‑M2/128‑148 (MQ=255)
                      tgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGt                                                                                                                                    >  8:221973‑M2/127‑128 (MQ=255)
                        ggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaata                                                                                                                <  8:562051‑M1/146‑20 (MQ=255)
                        cgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCAc                                                                                                              <  7:207231‑M2/24‑1 (MQ=255)
                         gaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCAccc                                                                                                            >  5:514844‑M2/124‑149 (MQ=255)
                         gCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatgga                                                                                                            <  8:58837‑M1/149‑24 (MQ=255)
                             aaCTCCTCTGGATCATTACTCACTGATCCTTGACACCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGATTCTCCTCTTTTATCAATATGGGTTGTTATCAAATCGTTACGCGATGATtcatactacacacgaaatatggaca                                                                                                          >  5:135681‑M1/1‑122 (MQ=255)
                                   tctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCTTTACGAGCTGTTtgatcctccccacgtaatatggacccaggcct                                                                                                   >  6:372852‑M1/1‑116 (MQ=255)
                                   cgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAAt                                                                                                  <  5:83956‑M2/36‑1 (MQ=255)
                                       gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagc                                                                                               >  6:358556‑M1/1‑112 (MQ=255)
                                             tACTCACTCATCCTTGACCCCGCTGCGGCGGGGTTTTCATTTCCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTCTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggccaaagcgagattc                                                                                        >  6:468630‑M1/1‑106 (MQ=255)
                                                   cTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttt                                                                                  <  2:237903‑M1/149‑50 (MQ=255)
                                                    tGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgtttt                                                                                 >  2:177809‑M1/1‑99 (MQ=255)
                                                         cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaagatggacacaggcctaagcggggttcttgttttcaaat                                                                            >  7:199672‑M1/1‑94 (MQ=255)
                                                            ctggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTaa                                                                         <  2:305863‑M2/61‑1 (MQ=255)
                                                             aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgt                                                                         <  5:358556‑M1/148‑59 (MQ=255)
                                                              ccccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttc                                                                       <  2:282155‑M1/149‑61 (MQ=255)
                                                                ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccg                                                                     <  1:177809‑M1/149‑63 (MQ=255)
                                                                      ctgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTa                                                               >  3:250764‑M2/79‑149 (MQ=255)
                                                                             gTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgc                                                         >  3:48107‑M1/1‑74 (MQ=255)
                                                                              ttGTCATTTGCTTTGCTTCAAGGGTTATCCTCTTTTATCTATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggtcacaggcctaagcgaggttcttgttttcaaattgttccggtcttaggcctcca                                                       <  4:48107‑M1/149‑77 (MQ=255)
                                                                                 atgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAAc                                                     <  6:514844‑M2/81‑1 (MQ=255)
                                                                                      tgctttgaCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGGTACGCGATGTTtgatcctccccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaac                                                <  5:372852‑M1/148‑84 (MQ=255)
                                                                                       gctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactg                                              >  5:552490‑M1/1‑64 (MQ=255)
                                                                                        tcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTa                                                                          >  8:545584‑M2/61‑120 (MQ=255)
                                                                                        tcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTa                                                                          <  7:545584‑M2/60‑1 (MQ=255)
                                                                                                caAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaata                                                                                                                >  8:330042‑M1/1‑55 (MQ=255)
                                                                                                caAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaata                                                                                                                <  7:330042‑M1/74‑20 (MQ=255)
                                                                                                            tctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatgga                                                                                                            >  7:133014‑M1/1‑43 (MQ=255)
                                                                                                            tctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatgga                                                                                                            <  8:133014‑M1/66‑24 (MQ=255)
                                                                                                              ttttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaat                        >  6:341346‑M1/1‑41 (MQ=255)
                                                                                                              tcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAc                                                                                    >  5:430620‑M2/39‑88 (MQ=255)
                                                                                                              tcacttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAc                                                                                    <  6:430620‑M2/50‑1 (MQ=255)
                                                                                                                acttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgtcgtc                                                        <  2:383188‑M2/78‑1 (MQ=255)
                                                                                                                acttagccgcgtgtccactattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATgtcgtc                                                        >  1:383188‑M2/37‑114 (MQ=255)
                                                                                                                  aTCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacat                   >  5:51272‑M1/1‑37 (MQ=255)
                                                                                                                            gttgttATCAAATCGTTACGCGATTTTtgatcctaccgacgtaatatggacacaggcctaatcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataa          <  5:468630‑M1/148‑122 (MQ=255)
                                                                                                                                 ctattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCa                                                       >  7:540180‑M2/20‑98 (MQ=255)
                                                                                                                                 ctattgctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCa                                                       <  8:540180‑M2/79‑1 (MQ=255)
                                                                                                                                   tCAAATCGTTACGCGATGTTtgatcctacccacgtaatatggacacaggcctaagcgaggttcttgttttcaaattgttccggactgaggccgccacaccaactgtgccgccgccaccgattgtaatcacattcgatataattaaacac  >  2:13729‑M1/1‑20 (MQ=255)
                                                                                                                                       ctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc    >  4:74684‑M2/14‑143 (MQ=255)
                                                                                                                                       ctgggtaagatcaGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc    <  3:74684‑M2/130‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                   
GTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCA  >  NC_000913/1335613‑1335892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.