Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 483,017 T→A 5.6% T463S (ACT→TCT)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913483,0170TA 5.6% 335.0 / 5.0 103T463S (ACT→TCT) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (52/45);  new base A (2/4);  total (54/49)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTC  >  NC_000913/482870‑483164
                                                                                                                                                   |                                                                                                                                                   
agCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGt                                                                                                                                                     <  8:253063/148‑1 (MQ=255)
 gCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg                                                                                                                                                   <  5:103178/149‑1 (MQ=255)
  ccTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGa                                                                                                                                                  <  1:566071/149‑1 (MQ=255)
     tttttACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAAcc                                                                                                                                               >  1:136043/1‑149 (MQ=255)
           ccTTCCCCGTGATCTCCTTTGGCAATCGGTTTCAGCATGGTTGCACAAATAGCTGGAGTCAGGATCAACGCAACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAAATGACGATAGATAGCACCAGTAGAACCGACaaa                                                                                                                                         <  5:165557/149‑1 (MQ=255)
           ccTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa                                                                                                                                         >  7:511011/1‑149 (MQ=255)
              tCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGAAGAACCGcc                                                                                                                                            >  7:81040/1‑143 (MQ=255)
              tCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGAAGAACCGcc                                                                                                                                            <  4:25891/143‑1 (MQ=255)
              tCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGAAGAACCGcc                                                                                                                                            >  3:25891/1‑143 (MQ=255)
                cccGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAgg                                                                                                                                    >  8:549067/1‑149 (MQ=255)
                 ccGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc                                                                                                                                   >  2:135940/1‑149 (MQ=255)
                  cGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGc                                                                                                                                   >  2:42472/1‑148 (MQ=255)
                   gTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCa                                                                                                                                 <  2:518165/149‑1 (MQ=255)
                     gATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATc                                                                                                                               >  5:530030/1‑149 (MQ=255)
                         gCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                                                                                                           <  1:42472/149‑1 (MQ=255)
                         gCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa                                                                                                                           <  1:135940/149‑1 (MQ=255)
                          ccTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                                                                                                          <  3:487456/149‑1 (MQ=255)
                                        tttCAGCATGGTGGCACAAAGAGCTGGAGTCAGGCTCAACGCCACCAGTACCGACAGCGCCCTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATAGGTACGAATACCGCCGa                                                                                                              >  3:311136/1‑147 (MQ=255)
                                              cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                                                                                                                        <  1:137033/115‑1 (MQ=255)
                                              cATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag                                                                                                                                        >  2:137033/1‑115 (MQ=255)
                                               aTGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt                                                                                                     >  5:248091/1‑149 (MQ=255)
                                                tggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc                                                                                                    >  6:213180/1‑149 (MQ=255)
                                                    ggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa                                                                                                <  2:136043/149‑1 (MQ=255)
                                                       acaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                                                                                                          <  6:224719/120‑1 (MQ=255)
                                                       acaAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc                                                                                                                          >  5:224719/1‑120 (MQ=255)
                                                        caAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc                                                                                            >  4:109898/1‑149 (MQ=255)
                                                         atagagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc                                                                                                                    <  8:301815/122‑1 (MQ=255)
                                                         aaaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAc                                                                                                                    >  7:301815/1‑124 (MQ=255)
                                                           agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa                                                                                                                                                    <  3:326018/90‑1 (MQ=255)
                                                           agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTa                                                                                                                                                    >  4:326018/1‑90 (MQ=255)
                                                           agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc                                                                                         <  6:248091/149‑1 (MQ=255)
                                                             agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                                                                       <  3:272743/149‑1 (MQ=255)
                                                                  gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcg                                                                                  >  8:186332/1‑149 (MQ=255)
                                                                     tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc                                                                               >  1:267849/1‑149 (MQ=255)
                                                                       aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt                                                                              <  6:530030/148‑1 (MQ=255)
                                                                           tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAAt                                                                          >  1:213049/1‑148 (MQ=255)
                                                                           tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc                                                                         >  5:475592/1‑149 (MQ=255)
                                                                           tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc                                                                         >  1:346154/1‑149 (MQ=255)
                                                                             aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAc                                                                                             <  6:522093/127‑1 (MQ=255)
                                                                             aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAc                                                                                             >  5:522093/1‑127 (MQ=255)
                                                                             aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTg                                                                       >  8:141819/1‑149 (MQ=255)
                                                                               cGCCACCAGTACCGACAGCGCCATTGATGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAGGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                                                                     <  8:511011/149‑1 (MQ=255)
                                                                                  caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa                                                                                                            <  8:143027/107‑1 (MQ=255)
                                                                                   accaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCAt                                                                 >  6:95438/1‑149 (MQ=255)
                                                                                    ccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATc                                                                >  5:405629/1‑149 (MQ=255)
                                                                                     caGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGCTAGCTAGCACCAGTAGACCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg                                                               >  5:152866/1‑149 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa                                                                                                >  7:328189/1‑113 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa                                                                                                <  8:328189/113‑1 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc                                                                         <  7:188427/136‑1 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc                                                                         >  8:188427/1‑136 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt                                                            >  2:24079/1‑149 (MQ=255)
                                                                                        tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt                                                            >  6:454718/1‑149 (MQ=255)
                                                                                          ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                                                                     <  5:389021/138‑1 (MQ=255)
                                                                                          ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                                                                     >  6:389021/1‑138 (MQ=255)
                                                                                           cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc                                                         <  6:475592/149‑1 (MQ=255)
                                                                                           cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGAAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc                                                         <  2:213049/149‑1 (MQ=255)
                                                                                             aCAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAACACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACgg                                                       >  1:305841/1‑149 (MQ=255)
                                                                                              cAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg                                                      <  7:141819/149‑1 (MQ=255)
                                                                                               aGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGt                                                     <  5:213180/149‑1 (MQ=255)
                                                                                                  gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt                                                            <  7:190141/139‑1 (MQ=255)
                                                                                                  gcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt                                                            >  8:190141/1‑139 (MQ=255)
                                                                                                    cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc                                                                                                    >  5:328582/1‑97 (MQ=255)
                                                                                                    cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc                                                                                                    <  6:328582/97‑1 (MQ=255)
                                                                                                     aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc                                               >  3:215123/1‑149 (MQ=255)
                                                                                                         cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt                                           <  5:454718/149‑1 (MQ=255)
                                                                                                         cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt                                           >  1:294868/1‑149 (MQ=255)
                                                                                                         aagAAACAAAGGAAATAGACAACTCACCATAGAAAGCACCAGAAGAACCCCCAAAGAAGGCCAACCGAACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCAGCCCCATCGACTTACTGGTAGCTTCtttt                                           <  4:311136/147‑1 (MQ=255)
                                                                                                          tGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga                                                                                     >  1:37038/1‑106 (MQ=255)
                                                                                                          tGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga                                                                                     <  2:37038/106‑1 (MQ=255)
                                                                                                           gAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTgg                                         >  5:10350/1‑149 (MQ=255)
                                                                                                             aaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTTAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTg                                          <  8:172884/146‑1 (MQ=255)
                                                                                                             aaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTg                                          >  7:172884/1‑146 (MQ=255)
                                                                                                                aaTGGTAATAGAGAACTGACGATAGATAGCACCATTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAc                                                                                                             <  2:552983/76‑1 (MQ=255)
                                                                                                                aaTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAc                                                                                                             >  1:552983/1‑76 (MQ=255)
                                                                                                                aaTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcggc                                     >  3:26915/1‑148 (MQ=255)
                                                                                                                     tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGCGCGCCCTGAATCTGCCCCATCGAGTTAc                                                         >  6:183131/1‑123 (MQ=255)
                                                                                                                     tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGATCGCCCTGAATCTGCCCCATCGACTTAc                                                         <  5:183131/123‑1 (MQ=255)
                                                                                                                       agagagAACTTACGATAGATATCAACAGTAGAACCTCCAAAGCAGTCCATCGGTACGAATACCGCCGACACTACCATCGCGATACCGACCAGAGCGCACTGAATCTGCCCCCTAGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc                             <  1:450259/147‑1 (MQ=255)
                                                                                                                        tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt                                                                        >  6:250856/1‑105 (MQ=255)
                                                                                                                        tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt                                                                        <  5:250856/105‑1 (MQ=255)
                                                                                                                            gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATGCCGACCAGAGCGCCCTGAATCt                                                                        >  3:109228/1‑101 (MQ=255)
                                                                                                                            gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATGCCGACCAGAGCGCCCTGAATCt                                                                        <  4:109228/101‑1 (MQ=255)
                                                                                                                               cTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCa                     <  4:215123/149‑1 (MQ=255)
                                                                                                                                  aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc                                                         <  1:250746/110‑1 (MQ=255)
                                                                                                                                    gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                                                                       <  1:118437/78‑1 (MQ=255)
                                                                                                                                    gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                                                                       <  1:232444/78‑1 (MQ=255)
                                                                                                                                    gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                                                                       >  2:232444/1‑78 (MQ=255)
                                                                                                                                    gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa                                                                                       >  2:118437/1‑78 (MQ=255)
                                                                                                                                         atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGt                                                                                                          >  2:329281/1‑54 (MQ=255)
                                                                                                                                         atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGt                                                                                                          <  1:329281/54‑1 (MQ=255)
                                                                                                                                           agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                                                                     >  4:250710/1‑89 (MQ=255)
                                                                                                                                           agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc                                                                     <  3:250710/89‑1 (MQ=255)
                                                                                                                                            gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCaa         <  6:10350/148‑1 (MQ=255)
                                                                                                                                            gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc        <  3:109898/148‑1 (MQ=255)
                                                                                                                                             cACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg       >  5:414176/1‑149 (MQ=255)
                                                                                                                                               ccAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGtt     <  5:95438/149‑1 (MQ=255)
                                                                                                                                               ccAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGtt     <  2:267849/149‑1 (MQ=255)
                                                                                                                                                 aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGtttt   >  1:295731/1‑149 (MQ=255)
                                                                                                                                                  gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTc  <  2:346154/149‑1 (MQ=255)
                                                                                                                                                  gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTc  >  2:279860/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCCTTTTTTACCTTCCCCGTGATCGCCTTTGGCAATCGGTTTCAGCATGGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTC  >  NC_000913/482870‑483164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: