Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,714,255 T→C 8.0% intergenic (+25/+184) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,714,2550TC 8.0% 142.9 / 3.4 50intergenic (+25/+184)srmB/yfiEATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (18/28);  new base C (2/2);  total (20/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTC  >  NC_000913/2714110‑2714401
                                                                                                                                                 |                                                                                                                                                  
gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATACCAGGCTTAAACCTGGCAtt                                                                                                                                                 <  8:243381/149‑1 (MQ=255)
gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACC‑AAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATACCAGGCTTAAACCTGGCAtt                                                                                                                                                 >  6:118348/1‑148 (MQ=255)
 aaaaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACC‑AAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATACCAGGCTTAAACCTGGCAttga                                                                                                                                               <  5:118348/149‑3 (MQ=255)
           aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg                                                                                                                                      <  6:156987/149‑1 (MQ=255)
           aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTg                                                                                                                                       <  6:105188/148‑1 (MQ=255)
              agaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCa                                                                                                                                    >  7:109520/1‑148 (MQ=255)
                           gcgGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGcc                                                                                                                      >  8:62877/1‑149 (MQ=255)
                            cgGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCt                                                                                                                     <  2:98921/149‑1 (MQ=255)
                                tGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATg                                                                                                                 >  1:85074/1‑149 (MQ=255)
                                  aaaaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATgc                                                                                                                <  4:102638/148‑1 (MQ=255)
                                   aaaaaaGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAAACAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATgcg                                                                                                               <  6:136321/148‑1 (MQ=255)
                                   aaaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATgcgc                                                                                                              >  6:111549/1‑149 (MQ=255)
                                   aaaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATgcgc                                                                                                              >  4:36049/1‑149 (MQ=255)
                                    aaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                                                                                                                                               >  6:130835/1‑115 (MQ=255)
                                    aaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                                                                                                                                               <  5:130835/115‑1 (MQ=255)
                                    aaaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGc                                                                                                                                     <  1:206812/125‑1 (MQ=255)
                                     aaaCGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTa                                                                                                            <  7:62877/149‑1 (MQ=255)
                                        cGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGc                                                                                                         <  3:104695/149‑1 (MQ=255)
                                               gcgACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGGTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCa                                                                                                   <  2:181557/148‑1 (MQ=255)
                                                 gACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGc                                                                                                <  1:246803/149‑1 (MQ=255)
                                                       aaaaaTATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTTAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGt                                                                                          >  6:76696/1‑149 (MQ=255)
                                                       aaaaaTATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGt                                                                                          >  7:16359/1‑149 (MQ=255)
                                                         aaaTATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGATGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTgg                                                                                        <  2:24801/149‑1 (MQ=255)
                                                         aaaTATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTgg                                                                                        >  6:213720/1‑149 (MQ=255)
                                                          aaTATTGGTAAGCGCCGAAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTTATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGGGCTGGCATACGCAATACTGCCTTATGCGCTACGCTTATCAGGCCTACGTGGc                                                                                       <  4:27170/149‑1 (MQ=39)
                                                              ttGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTTCCACCGCATACGACAGATTAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCACACGCAAAACTGCCTGATGCGCTTCGCTTATCAGGCCTACGTGGCTCa                                                                                    <  6:64700/148‑1 (MQ=38)
                                                                 gTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGc                                                                                 >  3:229490/1‑148 (MQ=255)
                                                                      cgcCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatata                                                                           <  8:16359/149‑1 (MQ=255)
                                                                             aaCCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAt                                                                     <  5:213720/148‑1 (MQ=255)
                                                                              aCCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAAttt                                                                   <  3:86921/149‑1 (MQ=255)
                                                                                  aGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCAc                                                               <  3:36049/149‑1 (MQ=255)
                                                                                      gAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGAAATATATTGaa                                                                      <  6:37009/138‑1 (MQ=255)
                                                                                      gAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGAAATATATTGaa                                                                      >  5:37009/1‑138 (MQ=255)
                                                                                           ggCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTa                                                      <  5:76696/149‑1 (MQ=255)
                                                                                                 ccACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg                                                 >  7:94936/1‑148 (MQ=255)
                                                                                                  cACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGa                                               <  2:85074/149‑1 (MQ=255)
                                                                                                  cACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGa                                               >  3:228711/1‑149 (MQ=255)
                                                                                                    ccGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatat                                                                            <  3:81447/118‑1 (MQ=255)
                                                                                                    ccGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAatat                                                                            >  4:81447/1‑118 (MQ=255)
                                                                                                        aatcGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc                                         <  4:105823/146‑1 (MQ=255)
                                                                                                          aCGACAGAAGAGTAATCTCAATGACAGGTTTACGCCTGGTATTAAAAGTGCTGGCAACCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt                                       <  1:67585/149‑1 (MQ=255)
                                                                                                           cGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt                                      <  5:111549/149‑1 (MQ=255)
                                                                                                               agaagaGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg                                  >  1:31244/1‑149 (MQ=255)
                                                                                                                 aagaGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgcc                                >  5:178265/1‑149 (MQ=255)
                                                                                                                 aagaGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgcc                                >  3:195296/1‑149 (MQ=255)
                                                                                                                      cacttttaaTACCAGGCTTAAACCTGGCATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATc                           >  8:8706/8‑149 (MQ=18)
                                                                                                                            cAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTa                                                                                                            <  4:40526/62‑1 (MQ=255)
                                                                                                                            cAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTa                                                                                                            >  3:40526/1‑62 (MQ=255)
                                                                                                                                   aGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATaaa              <  4:228711/149‑1 (MQ=255)
                                                                                                                                                gTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc  <  4:195296/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTC  >  NC_000913/2714110‑2714401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑