Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,236,716 C→T 6.0% N59N (AAC→AAT ygjQ → DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,7160CT 6.0% 206.4 / 2.7 66N59N (AAC→AATygjQDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (30/32);  new base T (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCAT  >  NC_000913/3236570‑3236858
                                                                                                                                                  |                                                                                                                                              
cGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGCCCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGt                                                                                                                                              >  6:105187/1‑149 (MQ=255)
cGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGt                                                                                                                                              <  5:164173/149‑1 (MQ=255)
cGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGt                                                                                                                                              >  2:172133/1‑149 (MQ=255)
  tATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTgg                                                                                                                                            <  3:228128/149‑1 (MQ=255)
    tttGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGAGCATCCTTATCGCAGCTCGCTTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTggga                                                                                                                                          <  6:75980/149‑2 (MQ=255)
         ttttCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGCTCCGGCGCGTAACGTGGGTTTAt                                                                                                                                      <  1:142764/148‑1 (MQ=255)
                gCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTg                                                                                                                                 <  1:172133/146‑1 (MQ=255)
                    gACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGa                                                                                                                          <  8:30355/149‑1 (MQ=255)
                         tAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAATGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     >  6:150704/1‑149 (MQ=255)
                         tAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAATGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     >  6:147175/1‑149 (MQ=255)
                         tAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     >  7:15315/1‑149 (MQ=255)
                         tAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     >  8:195729/1‑149 (MQ=255)
                         tAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCGGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     >  2:52104/1‑149 (MQ=255)
                           aaGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCggg                                                                                                                   <  5:76555/149‑1 (MQ=255)
                           aaGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCggg                                                                                                                   <  3:108427/149‑1 (MQ=255)
                           aaGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCg                                                                                                                     <  7:56749/147‑1 (MQ=255)
                             gATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGt                                                                                                                  >  6:63081/1‑148 (MQ=255)
                              aTTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTaa                                                                                                                >  1:107039/1‑149 (MQ=255)
                               ttGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAAt                                                                                                               >  8:242023/1‑149 (MQ=255)
                                       ttACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACtt                                                                                                       >  7:90533/1‑149 (MQ=255)
                                          cTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAgg                                                                                                                        >  3:208541/1‑129 (MQ=255)
                                          cTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAgg                                                                                                                        <  4:208541/129‑1 (MQ=255)
                                          cTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGGCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTAc                                                                                                    <  6:205663/149‑1 (MQ=255)
                                           tGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTAcc                                                                                                   >  4:63190/1‑149 (MQ=255)
                                             ttACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGCGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTAccc                                                                                                  >  3:34443/1‑148 (MQ=255)
                                                    tcgCAGGAGCGACCATCCTTCCCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGTGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTAtt                                                                                          <  5:199214/147‑1 (MQ=255)
                                                      gCAGGAGCGCCCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTACTCGCTACTTTACCCGCCGTATTg                                                                                         >  4:28320/1‑148 (MQ=255)
                                                      gCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCAGGAGCGATGTCAACGCCGTTCCGGCCACTAACGTGGCATTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGa                                                                                        >  7:173885/1‑149 (MQ=255)
                                                        aGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATa                                                                                      <  7:10543/149‑1 (MQ=255)
                                                         ggAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATAc                                                                                     >  6:54563/1‑149 (MQ=255)
                                                                   tCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCg                                                                           >  1:167442/1‑149 (MQ=255)
                                                                     cTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCCGCAGCGtt                                                                         <  5:63081/149‑1 (MQ=255)
                                                                     cTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGtt                                                                         <  7:195729/149‑1 (MQ=255)
                                                                        aTCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCg                                                                                                                           <  3:16855/96‑1 (MQ=255)
                                                                        aTCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCg                                                                                                                           >  4:16855/1‑96 (MQ=255)
                                                                          cGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTAc                                                                     >  4:40476/1‑148 (MQ=255)
                                                                            ccGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCAc                                                                  <  2:167442/149‑1 (MQ=255)
                                                                              gATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGc                                                                >  7:59410/1‑149 (MQ=255)
                                                                              gATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGc                                                                <  2:107039/149‑1 (MQ=255)
                                                                               atagcgTAATGGTCAACGACAGCAAACAGCTGACATGGAGCGATGTCAACGACGTTCCGGCGAGTAACGTTGGTTTATTGCTGGGGGAGATGCCGGGTAATAGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTTTACCACGcc                                                               >  5:214687/4‑149 (MQ=255)
                                                                                    gTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAAGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTAc                                                                     >  6:186524/1‑138 (MQ=255)
                                                                                    gTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAAGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTAc                                                                     <  5:186524/138‑1 (MQ=255)
                                                                                     tAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAATGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGaaaa                                                         <  5:150704/149‑1 (MQ=255)
                                                                                     tAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAATGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGaaaa                                                         <  5:147175/149‑1 (MQ=255)
                                                                                     tAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGaaaa                                                         <  8:15315/149‑1 (MQ=255)
                                                                                     tAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGaa                                                           >  3:212256/1‑147 (MQ=255)
                                                                                      aaTGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAAt                                                                                                               <  3:168243/94‑1 (MQ=255)
                                                                                          gTCAACGCCAGTAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGa                                                     >  6:233247/1‑148 (MQ=255)
                                                                                          gTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGaa                                                    <  5:54563/149‑1 (MQ=255)
                                                                                             aacgaatgCACACAGCTGTCCTGGATCTATGTCAAGGCCTTTCCTGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGGTACTTTACCCGTCGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAAt                                                  <  8:173885/141‑1 (MQ=255)
                                                                                              aCGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATgg                                                >  7:79651/1‑149 (MQ=255)
                                                                                              aCGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATgg                                                <  6:216666/149‑1 (MQ=255)
                                                                                                     cAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTgg                                         >  4:157365/1‑149 (MQ=255)
                                                                                                      aaaCAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTgg                                         >  3:213596/1‑148 (MQ=255)
                                                                                                          aGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGt                                    >  5:158611/1‑149 (MQ=255)
                                                                                                            cTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTgg                                  <  5:76997/149‑1 (MQ=255)
                                                                                                                   ggAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAAc                           >  7:135337/1‑149 (MQ=255)
                                                                                                                    gAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACg                          <  5:105187/149‑1 (MQ=255)
                                                                                                                              aaCGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACGACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTaaaaa                <  8:59410/149‑1 (MQ=255)
                                                                                                                                 gCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTa             <  3:63190/149‑1 (MQ=255)
                                                                                                                                  ccGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTAc            <  3:51297/149‑1 (MQ=255)
                                                                                                                                   cGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGctgctg                                          <  6:141710/118‑1 (MQ=255)
                                                                                                                                   cGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGctgctg                                          >  5:141710/1‑118 (MQ=255)
                                                                                                                                   cGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACg           <  5:233247/149‑1 (MQ=255)
                                                                                                                                        cGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGAAACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGaa      <  7:88931/149‑1 (MQ=255)
                                                                                                                                            gcgTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACAGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGAAAGTGGCGATAACTGACGTAAAAATTACGATGAAGCAt  >  3:25958/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCAT  >  NC_000913/3236570‑3236858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: