Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,239 G→C 10.7% noncoding (354/377 nt) rnpB ← RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,2390GC10.7% 120.0 / 7.4 46noncoding (354/377 nt)rnpBRNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base G (23/18);  new base C (2/3);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACC  >  NC_000913/3270107‑3270382
                                                                                                                                    |                                                                                                                                               
gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCATTCATCGTTGGGCCGCGGCAATAGCAAAAACCCGCCGAAGCGGGGGTTTACGTATATGAGGTGAAAGTGGCCGATAGGCCGGGGTGTGTCGTgggctg                                                                                                                                 >  4:19912/1‑145 (MQ=9)
          gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                                                                                                                 <  2:230671/123‑1 (MQ=11)
               gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGc                                                                                                                   >  5:102051/1‑148 (MQ=255)
                       ctttTGTGCATACCGGCGCTGACAGTACTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                                                                                           <  7:188584/148‑1 (MQ=18)
                          ttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCt                                                                                                        >  1:246781/1‑148 (MQ=255)
                              gTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCACCAAAAACAAAAACCCCCCGCAGCGGGTTTTTACGTAAATCACGTTAAACTGCCCCATAAGCCGGGTAATGTCCTGGACAGTCATTAATCTTGGCCCGCAAACGCtacaat                                                                                                   >  6:231589/1‑144 (MQ=17)
                                        gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                                                                                                                              >  5:5038/1‑96 (MQ=38)
                                        gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                                                                                                                              <  6:5038/96‑1 (MQ=38)
                                         tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                                                                                                                    >  3:179218/1‑105 (MQ=255)
                                         tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                                                                                                                    <  4:179218/105‑1 (MQ=255)
                                              aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc                                                                                   >  6:162151/1‑149 (MQ=255)
                                                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                                                                                                                          >  7:204834/1‑90 (MQ=255)
                                                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                                                                                                                          <  8:204834/90‑1 (MQ=255)
                                                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg                                                                               >  2:201927/1‑149 (MQ=255)
                                                   ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                                                                                                                              <  7:214051/85‑1 (MQ=39)
                                                   ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                                                                                                                              >  8:214051/1‑85 (MQ=39)
                                                        cTTTCTGCGCATCCATAAGCATAAACCCGTAGACGAGTGTTTTTACGTAAATGAGGCGAATCTGACCGCTATGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc                                                                            <  3:139199/146‑1 (MQ=255)
                                                         tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa                                                                        >  3:118199/1‑149 (MQ=255)
                                                              gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAGCGATAAGCCGGGTTCTGTCGGGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc                                                                   >  3:174590/1‑149 (MQ=255)
                                                                cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTgaa                                                                                                                                    >  8:9680/1‑80 (MQ=11)
                                                                  cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTa                                                               >  1:162352/1‑149 (MQ=255)
                                                                   tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTtttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagcaaaaacccgcttcggcgggtttttgct                                                              <  1:59756‑M2/149‑79 (MQ=255)
                                                                           taaaaaCCCGCTTCGGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                                                                                                                              >  6:73216/2‑77 (MQ=18)
                                                                           taaaaaCCCGCTTCGGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                                                                                                                              <  5:73216/76‑1 (MQ=18)
                                                                           cAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGaa                                                      >  5:164251/1‑149 (MQ=255)
                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAc                                                     >  6:188274/1‑149 (MQ=255)
                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAc                                                     >  2:87269/1‑149 (MQ=255)
                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTtttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagtaaaaacccgcttcggcgggtttttgcttttggaggg                                                     <  4:101478‑M2/149‑88 (MQ=255)
                                                                                   cgccgAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTAtt                                              <  1:194694/149‑1 (MQ=255)
                                                                                    gccgAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTAttt                                             >  4:146165/1‑149 (MQ=255)
                                                                                     ccgAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTg                                            <  1:117495/149‑1 (MQ=255)
                                                                                       gAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGc                                          <  4:118199/149‑1 (MQ=255)
                                                                                         aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTtttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagcaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaa                                        >  2:59756‑M2/1‑49 (MQ=255)
                                                                                          gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTtttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaat                                       >  3:101478‑M2/1‑48 (MQ=255)
                                                                                            gggTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGc                                     <  4:140763/149‑1 (MQ=255)
                                                                                                     cGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTg                             <  6:164251/148‑1 (MQ=255)
                                                                                                      gTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGa                           <  5:162151/149‑1 (MQ=255)
                                                                                                       tAAATCAGGTGAAACTGACCGATAAGCCGCGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAg                          <  2:162352/149‑1 (MQ=255)
                                                                                                         aaTCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGtt                        >  7:141620/1‑149 (MQ=255)
                                                                                                          aTCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGttt                       <  6:241590/149‑1 (MQ=255)
                                                                                                              ggTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCg                   >  1:146615/1‑149 (MQ=255)
                                                                                                               gTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGt                  >  1:235348/1‑149 (MQ=255)
                                                                                                                tGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTg                 <  3:146165/149‑1 (MQ=255)
                                                                                                                       gACCTATAAGCGGGGTGCGGTCGTGGACAGTCATTCATCTAGGGCAGGAATCGCTCACTGGCTCAAGCAGCCTCCCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGa          <  5:231589/149‑1 (MQ=255)
                                                                                                                        aCCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGa          <  1:87269/148‑1 (MQ=255)
                                                                                                                               aGCCGGGTTCTGTAGTGGTCAGTCATTCATCTAGGCACGAATTCGCTCACTGGGTCAAGCAGCCTACAGGGGTTCAGAAGGGGCCGTACCTTATGAACCCCTATTTTGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTAcc  <  4:174590/149‑1 (MQ=255)
                                                                                                                                gCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGACGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTAc   >  1:145406/1‑147 (MQ=255)
                                                                                                                                    |                                                                                                                                               
GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACC  >  NC_000913/3270107‑3270382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑